Volume 23 Issue 1
Feb.  2014
Turn off MathJax
Article Contents
Abstract
References
Related Articles
Chen Yueqin, He Jiawan, Zhuang Li, Zeng Longmei, Qu Lianghu. SEQUENCE ANALYSIS OF rDNA 16S-23S INTERGENIC SPACER FROM MICROCYSTIS AERUGINOSA AND MICROCYSTIS WESENBERGII IN DONGHU LAKE, CHINA[J]. ACTA HYDROBIOLOGICA SINICA, 1999, 23(1): 41-46.
Citation: Chen Yueqin, He Jiawan, Zhuang Li, Zeng Longmei, Qu Lianghu. SEQUENCE ANALYSIS OF rDNA 16S-23S INTERGENIC SPACER FROM MICROCYSTIS AERUGINOSA AND MICROCYSTIS WESENBERGII IN DONGHU LAKE, CHINA[J]. ACTA HYDROBIOLOGICA SINICA, 1999, 23(1): 41-46.
shu

SEQUENCE ANALYSIS OF rDNA 16S-23S INTERGENIC SPACER FROM MICROCYSTIS AERUGINOSA AND MICROCYSTIS WESENBERGII IN DONGHU LAKE, CHINA

  • Zhongshan University, Guangzhou 510275;
    2. Institute of Hydrobiology, The Chinese Academy of Seiences, Wuhan 430072

  • Received Date: September 09, 1998
  • Published Date: January 24, 1999
    Graphical Abstract
    • Abstract
  • The rDNA 16S-23S intergehic spacer regions from Microcytis aeruginosa and Microcystis wesenbergii in Donghu Lake of China were sequenced and analyzedto illustrate the evolutionary affiliations between toxic and nontoxic strains in genus.The rDNA spacer sequences ranged from 360bp to 364bp exclusive of 16S and 23SrDNA coding regions, containing a Ile tRNA gene. Sequence comparisons bycomputer programs showed that distance values between two species were about 0.05,whereas distance values between two toxic strains of Microcystis aeruginosa (8641 and7820) were 0.04, which indicated Microcystis aeruginosa and Microcystiswesenbergiihas a quite close relationship. In addition, Sequence comparisons showed the variationsin the position 282-295bp were high between tWo species. High diversity of thisregion may facilitate the design of DNA probes specific for toxic Microcystis aeruginosa in Denghu Lake of China. It is the first sequence report of rDNA 16S-23Sintergenic spacer regions for Microrystis, and the sequences will serve as the basis forthe indepth studies on the phylogeny of Microcystis.
    • FullText(HTML)
    • References (8)
  • [1]
    何家菀, 何振荣, 俞家禄, 等.东湖铜绿微囊藻毒素的分离与鉴定.海洋与湖沼, 1988, 19(5)：424-430
    [2]
    Carmichaed W W. A status report on planktonic Cyanobacteria (blue－green algae) and their toxins. Environmental Monitoring Systems Laboratory, Office of Research and Development, VS. Environmental Protection Agency, Cincinnati, Ohio, 1992
    [3]
    Infante A, Riechl W. The effect of cyanophyta upon zooplankton in a eutrophic tropical Lake. Hydrobiology, 1984, 113(29):293-298
    [4]
    Komarek J, Anagnostidis K. Modern approch to the classification system of cyanophytes 4 Nostocales. Arch. Hydrobiol. Suppl. 1989, 82.3 Algol. Stud. 56:247-345
    [5]
    Neilan B A, Jacobs D, Del Dot T, Blackall L L, Hawkins P R, Cox P T, Goodman A E. rRNA sequences and evolutionary relationships among toxic and nontoxic cyanobacteria of the genus Microcystis. Int J Syst Bacteriol. 1997, 47(3):693-697
    [6]
    Jensen M A, Hubner R J. Use of homoduplex ribosomal DNA spacer amplification products and heteroduplex cross-hybridization products in the identification of Salmonella serovars. Appl Environ Microbiol, 1996, 62(8):2741-2746
    [7]
    陈月琴, 屈良鹄 等.甲藻单个细胞DNA的制备及在赤潮藻类分子鉴定中的应用.中山大学学报(自然科学 版),1997, 36(4)：66-69
    [8]
    J萨姆布鲁克EF弗里奇T曼尼阿蒂斯.分子克隆实验指南(第二版).北京：科学出版社, 1996
    • Related Articles

  • Related Articles

    [1]CHEN Jia-Xin, CAI Fang-Fang. ALLOCOLEOPSIS (COLEOFASCICULALES, CYANOBACTERIA), A NEW RECORD GENUS OF TERRESTRIAL CYANOBACTERIA IN CHINA[J]. ACTA HYDROBIOLOGICA SINICA, 2025, 49(6): 062517. DOI: 10.7541/2025.2024.0323
    [2]ZHAO Ya-Jie, LI Guo-Qing, WANG Ye-Ping, JIN Qi-De, DENG Dao-Gui. SCREENING AND RNA INTERFERENCE STUDY OF TWO GENES RELATED TO REPRODUCTION IN DAPHNIA SINENSIS[J]. ACTA HYDROBIOLOGICA SINICA, 2024, 48(1): 176-184. DOI: 10.7541/2023.2023.0107
    [3]CHEN Ming, ZHOU Xie-Fei, GAO Jian-Jun, ZHU Ting-Yao, PENG Jia-Cheng, XU Hai-Sheng. CLONING AND FUNCTIONAL ANALYSIS OF STING IN CHINESE SOFT-SHELLED TURTLE (PELODISCUS SINENSIS)[J]. ACTA HYDROBIOLOGICA SINICA, 2022, 46(3): 324-331. DOI: 10.7541/2022.2021.133
    [4]LI Chun, CHEN Cheng, ZHAO Jian, HONG Xiao-You, LI Wei, ZHU Xin-Ping. SNPS DETECTION OF GHRL GENE AND ITS ASSOCIATION WITH GROWTH TRAITS OF CHINESE SOFT-SHELL TURTLE (PELODISCUS SINENSIS)[J]. ACTA HYDROBIOLOGICA SINICA, 2018, 42(2): 307-312. DOI: 10.7541/2018.039
    [5]SUN Lu-Ming, YU Meng-Jun, CHEN Ya-Dong, CHEN Xue-Jie, LIU Yang, QIU Xue-Mei, SHA Zhen-Xia. AKT-INTERACTING PROTEIN GENE CLONING AND ITS EXPRESSION PROFILE IN RESPONSE TO PATHOGEN INFECTION IN HALF SMOOTH TONGUE SOLE (CYNOGLOSSUS SEMILAEVIS)[J]. ACTA HYDROBIOLOGICA SINICA, 2016, 40(3): 467-473. DOI: 10.7541/2016.62
    [6]HUANG Xiao-Hong, CHEN Hong-Hui, HUANG Yi-Fan. PRELIMINARY STUDIES ON ISOLATION, PURIFICATION AND SOME PROPERTIES OF THE β-N-ACETYL-D-GLUCOSAMINIDASE FROM ERIOCHEIR SINENSIS[J]. ACTA HYDROBIOLOGICA SINICA, 2007, 31(4): 563-569.
    [7]XIE Zhi-Gang, NIU Cui-Juan. EFFECTS OF PARTIAL AND COMPLETE FOOD DEPRIV ATION ON COMPENSATORY GROWTH OF JUVENILE SOFT -SHELLED TURTLE ( PELODISCUS SINENSIS):TEMPORAL PATTERNS IN GROWTH RA TE AND CHANGES IN BODY COMPOSITION[J]. ACTA HYDROBIOLOGICA SINICA, 2007, 31(2): 214-219.
    [10]Liang Yan-lin, Chang Kuo-hsing. THE INNATE CAPACITY FOR NATURAL INCREASE OF DAPHNIA CARINATA KING[J]. ACTA HYDROBIOLOGICA SINICA, 1964, 5(1): 31-36.

Catalog

    Get Citation
    PDF
    XML
    Article views (976) PDF downloads (397) Cited by()
    Related

    Editorial Office of Acta Hydrobiologica Sinica

    Address：No. 7 Donghu South Road, Wuchang District, Wuhan, Hubei Province, China

    Tel：027-68780701；027-68780800 E-mail：acta@ihb.ac.cn  

    Supported byBeijing Renhe Information Technology Co. Ltd  Technical support：info@rhhz.net

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return