XU Xu-dong, KONG Ren-qiu, LIU Ke, WANG Jiang-xin, NING De-gang. PCR AMPLIFICATIONS OF FLANKING SEQUENCES OF ORFs IN THE GENOME OF SYNECHOCYSTIS SP. PCC6803 AND A STRATEGY FOR TARGETED GENE DISRUPTION[J]. ACTA HYDROBIOLOGICA SINICA, 2001, 25(6): 544-550.
Citation: XU Xu-dong, KONG Ren-qiu, LIU Ke, WANG Jiang-xin, NING De-gang. PCR AMPLIFICATIONS OF FLANKING SEQUENCES OF ORFs IN THE GENOME OF SYNECHOCYSTIS SP. PCC6803 AND A STRATEGY FOR TARGETED GENE DISRUPTION[J]. ACTA HYDROBIOLOGICA SINICA, 2001, 25(6): 544-550.
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PCR AMPLIFICATIONS OF FLANKING SEQUENCES OF ORFs IN THE GENOME OF SYNECHOCYSTIS SP. PCC6803 AND A STRATEGY FOR TARGETED GENE DISRUPTION

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  • The flanking sequences of 1927 potential protein-coding genes of Synechocystis sp. PCC6803 were amplified by polymerase chain reactions. The PCR products for the sll0267-sll0268-sll0269 region in the genomes of 4 substrains were inconsistent with those predicted by Kazusa DNA database. As shown with chlorophyll synthesis genes chlH and chlL as the examples, products of 3-piece-ligation PCR can be effectively applied to targeted gene disruptions in the genome of Synechocystis sp. PCC6803.
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