PCR AMPLIFICATIONS OF FLANKING SEQUENCES OF ORFs IN THE GENOME OF SYNECHOCYSTIS SP. PCC6803 AND A STRATEGY FOR TARGETED GENE DISRUPTION

The flanking sequences of 1927 potential protein-coding genes of Synechocystis sp. PCC6803 were amplified by polymerase chain reactions. The PCR products for the sll0267-sll0268-sll0269 region in the genomes of 4 substrains were inconsistent with those predicted by Kazusa DNA database. As shown with chlorophyll synthesis genes chlH and chlL as the examples, products of 3-piece-ligation PCR can be effectively applied to targeted gene disruptions in the genome of Synechocystis sp. PCC6803.