CYTOCHEMICAL LOCATION OF SUPEROXIDE DISMUTASE AND PEROXIDASE IN DIFFERENT TISSUES OF LITOPENAEUS VANNAMEI

XU Yan; YANG Jie; SUN Jing-Qiu; YU Ya-Dong; YE Jun-Qiang

Volume 34 Issue 2

Aug. 2014

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ACTA HYDROBIOLOGICA SINICA > 2010 > 34(2): 402-409.

XU Yan, YANG Jie, SUN Jing-Qiu, YU Ya-Dong, YE Jun-Qiang. CYTOCHEMICAL LOCATION OF SUPEROXIDE DISMUTASE AND PEROXIDASE IN DIFFERENT TISSUES OF LITOPENAEUS VANNAMEI[J]. ACTA HYDROBIOLOGICA SINICA, 2010, 34(2): 402-409.

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CYTOCHEMICAL LOCATION OF SUPEROXIDE DISMUTASE AND PEROXIDASE IN DIFFERENT TISSUES OF LITOPENAEUS VANNAMEI

College of Life and Environment Sciences, Shanghai Normal University, Shanghai 200234, China;
2. Shanghai Fishery Technical Extension Center, Shanghai 201400, China

Received Date: July 17, 2008
Rev Recd Date: May 25, 2009
Published Date: March 24, 2010

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Abstract

Superoxide dismutase (SOD) and peroxidase (POD) are two important anti- oxidation enzymes that exist widely in tissues of aerobic and anaerobic creatures. SOD and POD have been taken as two immune enzymes and a marker with several other related enzymes in evaluating crustacean animals’ immune function. Litopenaeus vannamei is Chinese major raised aquatic shrimp, and Virus is a major reason for the spreading of epidemic Litopenaeus vannamei diseases. To improve the own immunity of Litopenaeus vannamei and enhance its anti-virus function become the basic method of comprehensive prevention and cure. Therefore, people pay more and more attention to study the immunity factors of Litopenaeus vannamei and improve their anti-diseases ability effectively.The activities and location of superoxide dismutase (SOD) and peroxidase (POD) in the liver, muscle, heart, eye and gill of Litopenaeus vannamei were studied with enzyme cytochemical technique of electronic microscope, compared with the infected shrimp. The results showed that SOD positive reactions were mainly deposited in mitochondrium membrane and cytoplasm in the cells of the liver, muscle, heart and gill of the healthy shrimp. Furthermore, SOD activity was located around lipid droplet in liver. POD positive reactions were mainly deposited in specific organelle: peroxisome in the cells of the liver, heart and gill respectively. There were also POD positive granules around lipid droplet in liver. In the infected shrimp, the obviously histopathological changes were detected in five tissues, and more myelin figures appeared in the cell of each tissue and the number of the fat drops in liver cells sharply reduced. There were evident differences in tissues between diseased shrimp and healthy shrimp in the activities and location of SOD and POD. SOD positive granules in cytoplasm of the muscle, heart and gill of the infected shrimp disappeared; and the activities of SOD in liver decreased. At the same time, SOD positive reactions were also found in mitochondrial matrix of heart and gill. POD positive reactions were still found in peroxisome of the liver, heart and gill. But the number of peroxisome positive reactions in liver and gill got decreased; POD positive granules around lipid droplet in liver almost disappeared; part of peroxisomes in heart disintegrated and lots of positive-reacting granules scattered in cytoplasm.The results indicated that the activities and distribution of SOD and POD isozyme in Litopenaeus vannamei cells had clear tissue-specific, and SOD and POD played an important role in defense oxygen toxicity injury and the immune function of Litopenaeus vannamei. Our study will provide theoretical foundation for the application of immune technology in disease prevention and control in Litopenaeus vannamei in future.
- Litopenaeus vannamei,
- Virus,
- Superoxide dismutase (SOD),
- Peroxidase (POD),
- Cytochemical location

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[1]	Chen X C. The Shrimp’s Illness spread in the world and theevolvement on shrimp’s illness in china [J]. China Feed,1995, 11: 10—11 [陈宪春. 世界性虾病蔓延及我国虾病防治进展. 中国饲料, 1995, 11: 10—11].
[2]	Wang Y S, Qian L M. Nonspecific immune effect of threeimmune preparations as feed additives on pacific whiteshrimp, Litopenaeus vannamei [J]. Journal of Oceanographyin Taiwan Strait, 2006, 25(1): 41—47 [王永胜, 钱鲁闽. 三类免疫制剂对凡纳滨对虾非特异性免疫效应的研究, 台湾海峡, 2006, 25(1): 41—47].
[3]	He N H. The establishment and application of immune indexesof penaeid shrimp [J]. Journal of Xiamen University(Natural Science), 2004, 43(3): 385—388 [何南海. 对虾免疫功能指标的建立及其应用. 厦门大学学报(自然科学版),2004, 43(3): 385—388].
[4]	Lei Z W, Huang J, Liang C Z. Biological characters of whitespot syndrome virus (wssv) [J]. Marine Sciences, 2002,26(3): 26—31 [雷质文, 黄倢, 梁成珠. 白斑综合症病毒的生物学特性. 海洋科学, 2002, 26(3): 26—31].
[5]	Qiu L P, Li G Z, Zhu T Y. Electron microscopic observationof sod location in cardiac muscle cell [J]. Journal of ChineseElectron Microscopy Society, 1998, 17(3): 291—294 [邱兰萍, 李光芝, 朱天义. 心肌细胞内SOD 定位的电镜观察.电子显微学报, 1998, 17(3): 291—294].
[6]	Ke S S, Wang K M. Activity measurement and locationstaining of SOD and its isozyme in Rosaroxburghii juice [J].Acta Agriculturae Zhejiangensis, 2001, 13(3): 176—178 [柯世省, 王克满. 刺梨汁SOD 及其同工酶的活力测定与定位染色. 浙江农业学报, 2001, 13(3): 176—178].
[7]	Yao C L, Wang W N, Wang A L. Progress of studies on superoxidedismutase in the body of aquatic animals [J]. MarineSciences, 2003, 27(10): 18—21 [姚翠鸾, 王维娜, 王安利. 水生动物体内超氧化物歧化酶的研究进展, 海洋科学,2003, 27(10): 18—21].
[8]	Yang M H, Zheng Z, Jan E L. Histochemical and ultrastructuraldemonstration of peroxidase activity during infection ofrice by Magnaporthe grisea [J]. Chinese Journal of RiceScience, 2002, 16(1): 57—62 [杨民和, 郑重, Jan E. Lench.水稻受稻瘟病菌侵染后过氧化物酶定位的超微观察. 中国水稻科学, 2002, 16(1): 57—62].
[9]	Zhong C S. Enzyme tissue cytochemical technique [M].Shanghai: Shanghai Medical University Press. 1987 [钟慈声.酶组织细胞化学技术. 上海: 上海医科大学出版社. 1987].
[10]	Cao S H, Zha X D. Summary of the research on superoxidedismutase [J]. Journal of Anhui Agricultural Sciences, 2003,31(4): 599—601 [曹淑华, 查向东. 超氧化物歧化酶研究综述. 安徽农业科学, 2003, 31(4): 599—601].
[11]	Xu L H, Zhang Y Y and Chen Y Y. The advances of molecularecotoxicology and its significance in water environmentprotection [J]. Acta Hydrobiologica Sinica, 1995, 19(2):171—185 [徐立红, 张甬元, 陈宜瑜. 分子生态毒理学研究进展及其在水环境保护中的意义. 水生生物学报, 1995,19(2): 171—185].
[12]	Xiao X, Huang Z X, Zang E X, et al. Studies on purificationand characters of superoxide dismustase from Sinonovaculaconstricta [J]. Journal of Oceanography in Taiwan Strait,2002, 5: 172—177 [肖湘, 黄智璇, 张尔贤, 等. 益蛏超氧化物歧化酶的纯化和性质研究. 台湾海峡, 2002, 5:172—177].
[13]	Cai W Q. A study on pathology of the disease caused byTorulopsis mogll in giant freshwater prawn (Macrobrachiumrosenbergii) [J]. Journal of Fisheries of China, 1996, 20(1):13—17 [蔡完其. 罗氏沼虾莫格球拟酵母病的病理研究.水产学报, 1996, 20(1): 13—17].
[14]	Jia S J, Ying X P, Chen Y L, et al. A study on isozymeschanges during variant development periods of eriocheirsinensis [J]. Transaction of Oceanology and Limnology,2004, 4: 52—60 [贾守菊, 应雪萍, 陈艳乐, 等. 中华绒螯蟹不同发育阶段腹肢粘液腺同工酶的比较. 海洋湖沼通报, 2004, 4: 52—60].
[15]	Chang Y, Dai J J, Ma F M. The study of the activity in peroxidaseof first year bolting sugar beet induced by low temperatureand photoperiod [J] China Beet ＆ Sugar, 2002, 2:3—5 [常缨, 戴建军, 马凤鸣. 温光诱导甜菜当年抽薹过程中过氧化物酶的研究. 中国甜菜糖业, 2002, 2: 3—5].
[16]	Liu X Y, Zhang Z F, Bao Z M. The peroxisome in the tissuecell of Penaeus chinensis [J]. Marine Sciences, 2003, 27(11):43—46 [刘晓云, 张志峰, 包振民. 中国对虾组织细胞中的过氧化物酶体. 海洋科学, 2003, 27(11): 43—46].
[17]	Vogt G, Storch V, Quinitio E. Midgut gland as monitor organfor the nutritionalvalue of diets in penaeus monodon [J].Aquacul, 1985, 48: 1—12.
[18]	Chen K. The function of peroxidase in the animal [J]. BiologyBulletin, 1995, 30(10): 18—19 [陈康. 动物细胞中过氧化物酶体的功能. 生物学通报, 1995, 30(10): 18—19].

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