CRYOPRESERVATION OF DECAPSULATED ARTEMIA SP. EMBRYOS

The decapsulated Anemia sp.embryos at their blastula stage were successfullycryopreserved in liquid nitrogen with various concentrations of glycerol,DMSO,glucose andsucrose as single cryoprotectants. Higher cryoprotectant concentrations were better than thelowers Dual cryoprotectants (glycerol+DMSO, glucose+sucrose)had stronger cryoprotectionthan single-type cryoprotection. Saturated NaCl solution showed a powerful cryoprotpetionfor various developmental stages of Anemia sp. embryos,although a weaker cryoprotCctionwas observed for later stage.Intraembryoic ice crystal formation was the main reason causing the death of the embryos.The cryoprotection of various cryoprotectants is possibly related to their dehydration effect.