MICROSATELLITE MARKER ANALYSIS ON ARTIFICIALLY GYNOGENETIC PURE LINE OF RED-WHITE ORNAMENTAL CARP

One pure line of red white ornamental carp( Cyprinus carpio L.) was produced by two successive artificial gynogenesis with the combination treatment of UV irradiation sperm and cold shock (3-5℃) to block second polar body extrusion.The used heterozygous sperm came from bluntnose black bream( Megalobrama amblycephala Yih).Six microsatellite markers of the poly (CA) type isolated from common carp were used to assess genetic homogeneity among individuals of the artificially gynogenetic Red Write ornamental carp pure line.Electrophoretic patterns showed that the six pairs of microsatellite primers all produced well identifiable DNA fragments,and their size ranged from 100bp to 300bp.In this study,11 individuals similar to their mother in the color phenotype were selected from the artificially gynogenetic F1 progeny of Red-Write ornamental carps.The DNA fragment patterns of 11 individuals amplified by six primers were highly identical.One of them was successfully induced to spawn,and the ovulated eggs were used to produce artificial gynogenetic F2 progeny.Six individuals were randomly sampled from the F2 gynogenetic ornamental carps to analyze their genetic homogeneity.As a control,8 individuals reproduced from bisexual mating were also analyzed by the six microsatellite markers.The results indicated that the 6F2 gynogenetic individuals showed highly identical DNA patterns to their mother,and Mendel's genetic law of the six microsatellite markers were demonstrated by the control group.The current study provides a new technique to produce pure line in ornamental carp.Moreover,the artificially gynogenetic pure ling of red white ornamental carp will be useful for further studying on genetic mechanism of body color types in ornamental carp,and the molecular markers will be able to provide some valuable tools for marker-assisted selection breeding or gene mapping in the ornamental carp.