INACTIVATED VACCINE OBTAINTED THROUGH CELL CULTURE FOR HEMORRHAGE OF GRASS CARP(CTENOPHARYNGODON IDELLA):THE STABILITY OF VACCINE AND INFLUENCE OF ADJUVANTS AND BOOSTER IMMUNIATION ON THE IMMUNE RESPONSE

The stability of the vaccine is relatively high.After 8 months'storage at 4℃,the antiserum neutralization titre(ANT)were 119.24±8.8(2),and was not significantly different from that at start of storage period;the relative protection of grass carp immunized with the vaccine was 83.6%.After a storage period of 8 days at 28℃,the ANT and relative protection remained the same as those at start of the storage period.The incomplete Freund's adjuvant enhanced the immune effect of the vaccine.Both relative and ANT in the booster immunized grass carp were significantly different from those in the single immunized group.