WU Hong-yan, ZHAO Yi-jun, GUO Hou-liang, CHENG Kai, XU Min. CELL VACUOLIZATION INDUCED BY INORGANIC SALTS IN CYANOBACTERIUM[J]. ACTA HYDROBIOLOGICA SINICA, 2002, 26(3): 234-238.
Citation: WU Hong-yan, ZHAO Yi-jun, GUO Hou-liang, CHENG Kai, XU Min. CELL VACUOLIZATION INDUCED BY INORGANIC SALTS IN CYANOBACTERIUM[J]. ACTA HYDROBIOLOGICA SINICA, 2002, 26(3): 234-238.

CELL VACUOLIZATION INDUCED BY INORGANIC SALTS IN CYANOBACTERIUM

  • Received Date: November 14, 2001
  • Rev Recd Date: November 29, 2001
  • Published Date: May 24, 2002
  • Six cyanobaceria were cultured in the presence of NaCl、KNO3、(NH4)2SO4、MgSO4 and CaCl2, respectively for vacuole induction, and produced different effects. Anabaena sp. 595 was the most sensitive, vacuole could be induced by all salts tested. In contrast, no salts could induce cell vacuolization in Oscillatoria animlis 284 and Spirulina maxima 438 . While Nostoc sp. 96、Plectonema boryanum 246 and Scytonema hofmanni 248 were the relatively sensitive strains, only NaCl、KNO3、or(NH4)2SO4 revenaled the inducing effect on them. The induced vacuoles were examined by pressing the sample, and it was spherical and transparent in the scope of phase microscope.
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    Sinha R P, Haeder D.Response of a rice field cyanobacterium Anabaena sp.To physiological stressors [J]. Envir and Exper Botany, 1996,36: 147-155[2] Jeanjean R, Matthijs H C P, Onana B, et al. Exposure of the cyanobacterium Synechocystis PCC 6803 to salt stress induces concerted changes in respiration and photosynthesis [J]. Plant cell Physical, 1993, 34: 1073-1079[3] Schwartz S H, Black J A, Jager K, et al. Regulation of an osmoticum-responsive gene in Anabaena sp.Strain PCC 7120 [J]. J Bacteriol, 1998, 180(23): 6332-6337[4] Vinnemeier J, Hagemann M. Idetification of salt-regulated genes in the genome of the cyanobacterium Synechocystis sp.Strain PCC 6803 by subtractive RNA hybridization [J]. Arch Microbiol 1999, 172(6): 377-386[5] Blumwald B, Tel-or E. Structural aspects of the adaptation of Nostoc muscorum to salt [J]. Arch Microbiol. 1982, 132: 163-167[6] Stamatakis K, Ladas NP, Alygizaki-zorba A, et al. Sodiu m chloride-induced volume changes of freshwater cyanobacterium synechococcus sp.PCC 7942 cells can be probed by chlorophyll afluorescence [J]. Arch Bio Chem Biophys. 1999, 370(2): 240-249[7] 郭厚良. 青霉素-溶菌酶法分离蓝藻原生质球[J],植物学报,1990,32:510-513[8] 郭厚良,宋文贞,金传荫. 五属七种蓝藻原生质球分离研究[J],水生生物学报,1996,20:93-94[9] 郭厚良,金传荫,宋文贞. 无机盐对蓝藻细胞结构影响和原生质球形成[J],水生生物学报,1997,21:190-193[10] 郭厚良,黄开耀,易平. KCl诱导柱胞鱼腥藻形成液泡[J],水生生物学报,1998,22:198-199[11] 郭厚良,易平,浦秋文. 鱼腥藻sp.PCC 7120液泡的诱导和分离[J],武汉大学学报,2000(4):246-248

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