HE Shun-ping, WANG Wei, CHEN Yi-yu. THE RAPD ANALYSIS AND THE PHYLOGENETIC CONCERNING FOR PRIMITIVE CYPRINIDAE[J]. ACTA HYDROBIOLOGICA SINICA, 2000, 24(2): 101-106.
Citation: HE Shun-ping, WANG Wei, CHEN Yi-yu. THE RAPD ANALYSIS AND THE PHYLOGENETIC CONCERNING FOR PRIMITIVE CYPRINIDAE[J]. ACTA HYDROBIOLOGICA SINICA, 2000, 24(2): 101-106.

THE RAPD ANALYSIS AND THE PHYLOGENETIC CONCERNING FOR PRIMITIVE CYPRINIDAE

  • Received Date: January 19, 1999
  • Rev Recd Date: July 14, 1999
  • Published Date: March 24, 2000
  • Many informative fragments of the RAPD were obtained from 30 individuals that representing 20 species of the primitive cyprinid fishes by the method of random amplifying with 20 primers. We converted the DNA polymorphic fragment into genetic distance by the program Rapdplot (d=1-S, S=2NxNy/Nx+Ny). The prograxns Neighbor in software package PHYLIP was used to calculated the matrix of genetic distance resulted which in one cladogram of 20 species. It is indicated that the application of the RAPD analysis for Cyprinidae may have some limitations. For the inter-genera analysis in same subfamily, the result is reliable. The result of the RAPD analysis indicates that: the subfamily Danioinae is not a monophyletic group.Among them, the Zacco group and Aphyocypris group form monophyly respectively. And in this subfamily, the two groups do not have a direct relationship.
  • [1]
    陈湘粦等.鲤科的科下类群及宗系发生关系[J].动物分类学报,1984,9(4):424-440[2] Halward T. et al. Use of single-primer DNA;amplifications in genetic studies of peanut (Arachis hypogaea L.) [J]. Plant Nol. Biol., 1992, 18:315-325[3] 何舜平、汪亚平、陈宜瑜,1997五种鲤科鱼类RAPD分析兼论鲫的系统位置[J].水生生物学报,21(3):262-267[4] Auseul F M. Short protocols in Molecular Biology (2nd Ed) [M]. London: John Wiley Sons, 1992.[5] Willinames J G K, et al. DNA polymorhisms amplified by arbitary primers are useful as genetic as genetic markers[J]. Nucleic Acids Res., 1990,18(22):6531-6535[6] Page R D M Component[M], the natural history museum, London, 1993[7] 陈宜瑜等.珠江的鱼类区系及其动物地理区划的讨论[J].水生生物学报,1986,10(2):228-236[8] Demeke T, et al. Potential taxonomic use of random amplified polymorphic DNA (RAPD): a casestudy in Brassic[J]. Theor Appl Genet, 1992, 84:990-984[9] Kazan K, et al. Genetic relationships and variation in the Stylonsanthes guianensis species complex assessed by random amplified polymorphie DNA[J]. Genome, 1993,36(1):43-49[10] Williames C E, Clair A. St. Phenetic relationships and levels of variability detected by restrication fragment length polymorphism and random amplified polymorphic DNA analysis of cultivated and wild accession of Lycopersicon esculentum[J]. Genome, 1993,36(3):619-630

    陈湘粦等.鲤科的科下类群及宗系发生关系[J].动物分类学报,1984,9(4):424-440[2] Halward T. et al. Use of single-primer DNA;amplifications in genetic studies of peanut (Arachis hypogaea L.) [J]. Plant Nol. Biol., 1992, 18:315-325[3] 何舜平、汪亚平、陈宜瑜,1997五种鲤科鱼类RAPD分析兼论鲫的系统位置[J].水生生物学报,21(3):262-267[4] Auseul F M. Short protocols in Molecular Biology (2nd Ed) [M]. London: John Wiley Sons, 1992.[5] Willinames J G K, et al. DNA polymorhisms amplified by arbitary primers are useful as genetic as genetic markers[J]. Nucleic Acids Res., 1990,18(22):6531-6535[6] Page R D M Component[M], the natural history museum, London, 1993[7] 陈宜瑜等.珠江的鱼类区系及其动物地理区划的讨论[J].水生生物学报,1986,10(2):228-236[8] Demeke T, et al. Potential taxonomic use of random amplified polymorphic DNA (RAPD): a casestudy in Brassic[J]. Theor Appl Genet, 1992, 84:990-984[9] Kazan K, et al. Genetic relationships and variation in the Stylonsanthes guianensis species complex assessed by random amplified polymorphie DNA[J]. Genome, 1993,36(1):43-49[10] Williames C E, Clair A. St. Phenetic relationships and levels of variability detected by restrication fragment length polymorphism and random amplified polymorphic DNA analysis of cultivated and wild accession of Lycopersicon esculentum[J]. Genome, 1993,36(3):619-630

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