A NOVEL METHOD OF SCREENING NEUTRALIZING ANTIBODY AGAINST WHITE SPOT SYNDROME VIRUS USING PRIMARY CULTURED CELLS

According to the characteristics of affinity binding of virus with host cells, a novel method of screening neutralizing antibody against white spot syndrome virus(WSSV)has been established. After interaction of WSSV with primary cultured cells from shrimp lymphoid organ, unbound virus is washed away, and the cells are fixed by formaldehyde. Then the virus, which can bind on the cells, are assayed by ELISA. After incubation of virus with its antiserum, the antiserum that can inhibit virus binding ability to the cells should contain neutralizing antibody. This method has some strong points with handle easy and data stable, which can be used to screen neutralizing antibody under the lack of cell lines.