Volume 26 Issue 3
Feb.  2014
Turn off MathJax
Article Contents
Abstract
References
Related Articles
GAO Hong, YUAN Bi-feng, YUAN Li, ZHAO Xin-yan, DAI He-ping. A NOVEL METHOD OF SCREENING NEUTRALIZING ANTIBODY AGAINST WHITE SPOT SYNDROME VIRUS USING PRIMARY CULTURED CELLS[J]. ACTA HYDROBIOLOGICA SINICA, 2002, 26(3): 259-263.
Citation: GAO Hong, YUAN Bi-feng, YUAN Li, ZHAO Xin-yan, DAI He-ping. A NOVEL METHOD OF SCREENING NEUTRALIZING ANTIBODY AGAINST WHITE SPOT SYNDROME VIRUS USING PRIMARY CULTURED CELLS[J]. ACTA HYDROBIOLOGICA SINICA, 2002, 26(3): 259-263.
shu

A NOVEL METHOD OF SCREENING NEUTRALIZING ANTIBODY AGAINST WHITE SPOT SYNDROME VIRUS USING PRIMARY CULTURED CELLS

  • Institute of hydrobiology, The Chinese Academy of Sciences, Wuhan 430072

  • Received Date: August 31, 2001
  • Rev Recd Date: September 28, 2001
  • Published Date: May 24, 2002
    Graphical Abstract
    • Abstract
  • According to the characteristics of affinity binding of virus with host cells, a novel method of screening neutralizing antibody against white spot syndrome virus(WSSV)has been established. After interaction of WSSV with primary cultured cells from shrimp lymphoid organ, unbound virus is washed away, and the cells are fixed by formaldehyde. Then the virus, which can bind on the cells, are assayed by ELISA. After incubation of virus with its antiserum, the antiserum that can inhibit virus binding ability to the cells should contain neutralizing antibody. This method has some strong points with handle easy and data stable, which can be used to screen neutralizing antibody under the lack of cell lines.
    • FullText(HTML)
    • References (1)
  • [1]
    黄捷,宋晓玲,于佳,等. 杆状病毒的皮下及造血组织坏死-对虾爆发性病的病原和病理学[J]. 海洋水产研究1995,161:1-10[2] Wongteerasupaya C, Vickers J, Sriurairatana S, et al. A non-occluded systemic baculovirus origin and causes high mortalisty in the black tiger prawn penaeus monodon [J]. Diseases of Aquatic Organisms. 1995,21:69-77[3] Tapay L M, Lu Y, Gose R B, et al. Development of an in vitro quantal assay in primary cell cultures for a non-occluded baculo-like virus of penaeid shrimp [J]. J. Virol Method. 1997,64:37-41

    黄捷,宋晓玲,于佳,等. 杆状病毒的皮下及造血组织坏死-对虾爆发性病的病原和病理学[J]. 海洋水产研究1995,161:1-10[2] Wongteerasupaya C, Vickers J, Sriurairatana S, et al. A non-occluded systemic baculovirus origin and causes high mortalisty in the black tiger prawn penaeus monodon [J]. Diseases of Aquatic Organisms. 1995,21:69-77[3] Tapay L M, Lu Y, Gose R B, et al. Development of an in vitro quantal assay in primary cell cultures for a non-occluded baculo-like virus of penaeid shrimp [J]. J. Virol Method. 1997,64:37-41
    • Related Articles

  • Related Articles

    [1]Kadirdin Arken, GUO Ai-Min, HAO Cui-Lan, ZHANG Wen-Run, RONG Meng-Jie, SONG Peng-Hui, YUE Cheng. A NEW SPECIES OF DACTYLOGYRUS, D. DIPTYCHUS N. SP. (MONOGENEA: DACTYLOGYRIDAE), FROM SCHIZOTHORACINE FISHES (CYPRINIDAE: SCHIZOTHORACINAE) IN THE XINJIANG UYGUR AUTONOMOUS REGION, CHINA[J]. ACTA HYDROBIOLOGICA SINICA, 2020, 44(3): 663-669. DOI: 10.7541/2020.081
    [2]Shahid Mahboob, Bilal Hussain, Zahid Iqbal, Abdul Shakoor Chaudhry. ESTIMATION OF VOLATILE CONSTITUENTS IN THE FISH FLESH FROM WILD AND FARMED CIRRHINA MRIGALA AND CYPRINUS CARPIO[J]. ACTA HYDROBIOLOGICA SINICA, 2009, 33(3): 484-491.
    [7]Chang Jianbo, Cao Wenxuan. HISTORY AND PROSPECT OF CONSERVATION ON CHINESE STURGEON IN THE YANGTZE RIVER[J]. ACTA HYDROBIOLOGICA SINICA, 1999, 23(6): 712-720.
    [8]Wu Jihua, Liang Yanling, Sun Xida. NEWLY RECORDED SPECIES OF FREE-LIVING NEMATODES FROM CHINA (CHROMADORIDA,ENOPLIDA ARAEOLAIMIDA)[J]. ACTA HYDROBIOLOGICA SINICA, 1997, 21(4): 320-321.
    [9]Wu Weixin, Lin Linan, Xu Dayi. A TETRAPLOIDY HYBRID CROSSING RED CARP CYPRINUS CARPIO L. WITH GRASS CARP CTENOPHARYNGODEN IDELLA CUV. ET VAL.[J]. ACTA HYDROBIOLOGICA SINICA, 1981, 7(3): 433-436.
    [10]Lin Ding, Mao Yongqing, Cai Fasheng. EXPERIMENTS ON THE PROTEIN REQUIREMENTS OF GRASS CARP(CTENOPHARYNGODON IDELLUS (C. V.))JUVENILES[J]. ACTA HYDROBIOLOGICA SINICA, 1980, 4(2): 207-212.

Catalog

    Get Citation
    PDF
    XML
    Article views PDF downloads Cited by()
    Related

    Editorial Office of Acta Hydrobiologica Sinica

    Address：No. 7 Donghu South Road, Wuchang District, Wuhan, Hubei Province, China

    Tel：027-68780701；027-68780800 E-mail：acta@ihb.ac.cn  

    Supported byBeijing Renhe Information Technology Co. Ltd  Technical support：info@rhhz.net

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return