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YAN Jin-Peng, LIU Shao-Jun, SUN Yuan-Dong, ZHANG Chun, LIU Yun. RAPD ANALYSIS OF DIPLOID GYNOGEN POPULATIONS OF ALLOTETRAPLOID HYBRIDS OF RED CRUCIAN CARP(♀) X COMMON CARP(♂)[J]. ACTA HYDROBIOLOGICA SINICA, 2005, 29(3): 259-265.
Citation: YAN Jin-Peng, LIU Shao-Jun, SUN Yuan-Dong, ZHANG Chun, LIU Yun. RAPD ANALYSIS OF DIPLOID GYNOGEN POPULATIONS OF ALLOTETRAPLOID HYBRIDS OF RED CRUCIAN CARP(♀) X COMMON CARP(♂)[J]. ACTA HYDROBIOLOGICA SINICA, 2005, 29(3): 259-265.
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RAPD ANALYSIS OF DIPLOID GYNOGEN POPULATIONS OF ALLOTETRAPLOID HYBRIDS OF RED CRUCIAN CARP(♀) X COMMON CARP(♂)

  • Key Laboratory of Protein Chemistry and Fish Developmental Biology of Ministry of Education, College of life sciences, Hunan Normal University, Changsha 410081

  • Received Date: June 24, 2004
  • Rev Recd Date: September 26, 2004
  • Published Date: May 24, 2005
    Graphical Abstract
    • Abstract
  • Following activation by scatter scale carp sperm（a variety of common carp) that were UV-irradiated for 30 min, the diploid eggs stripped from F10female allotetraploid hybrids of red crucian carp（Carassius auratus red var.,♀) X common carp（Cyprinus carpio L.,♂), without or with the cold shock at0-4℃ for 30min, developed in normal live first generation diploidgynogens（G1).Interestingly, like the diploid F2hybrids, these diploid gynogens（G1) also produced diploid eggs. Without the treatment for doubling the chromosome number, the diploid eggs produced by the first generation gynogens, developed into the second-generation gynogens（G2) subjecting to activation by UV-irradiated scatter scale carp sperm. In the present paper, genetic heterogeneity and molecular markers were analyzed by RAPD technique in the first and second generation of artificial induced diploid gynogen population generated by the gynogenesis of F10allotetraploid hybrids. Of one hundred and thirty-four 10-nucleotide-long random primers used in the preliminary analysis, 53 primers produced wel-l amplified and reproducible band patterns.They were selected and used in the further analysis.The number of loci detected in the firs-t generation gynogen population and second-generation gynogen population were 541、 511, and the number of polymorphic loci were 70、 52, respectively.The percentage of polymorphic loci（12.94% )was considerately higher in the G1than that（10.18% ) of G2.The average genetic distances estimated byLynchp s index were 0.0732、 0.0464, respectively.Theresults showed that the genetic diversity of G2was significantly decreased after two continued generation gynogenesis, and the genetic purity of G2 was considerately higher than that of G1.Two primers, such sa S50、 S223, were observed to produce specific bands, and these bands could beused as molecularmarkers for discriminating the firs-t generation diploid gynogens fromthe second-generation diploid gynogens.A phylogenetictree which was constructed using Statistical Analysis System computer programme based on genetic distances clearly revealed that all individuals in the first generation gynogen population were clustered into one group,while all the individuals originated from the second-generation gynogen population were joined into another group.
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  • [1]
    Chourrout D. Tetraploidy induced by heat shock in the rainbow trout(Salmo gairdneri R.-[J]. Reprod. Nutr. Develop,1982, 22: 569-574
    [2]
    Refstie T.Tetraploid rainbow trout produced by cytochalas in B[J].Aquaculture, 1981, 25: 51-58
    [3]
    Myers J M.Tetraploid induction in Oreochro mis s pp [J]. Aquaculture,,1986,57:281-287
    [4]
    Hong Y H. Tetraploidy induced by heat shock in big head carp Aristichthys nobilis[J]. Acta Zoologica Sinica, 1990, 36(1-:70-75[洪云汉. 热休克诱导鳙鱼四倍体的研究. 动物学报, 1990, 36(1-:70-75]
    [5]
    Gui J F, Sun J M, Liang S C, et al.Studies on genome manipulation fish. Tetraploid induced by hytrostatic pressure treatment and a combination of hytrostatic pressure treatment and cold treatment in Transparent Colored Crucian Carp[J]. Acta Hydrobiologica Sinica,1991,15(4-:333-341. [桂建芳, 孙建民, 梁绍昌, 等. 鱼类色体组操作的研究. 静水压处理和静水压与冷休克结合处理导 水晶彩鲫四倍体. 水生生物学报,1991,15(4-:333-341]
    [6]
    Chen M R,Yang X Q,Yu X M, et al. Chromosome Ploidy manipulation of allotetraploids andtheir fertility in Japanese Phytophagous Crucian Carp and Red Crucian Carp[J]. Acta Hydrobiologica Sinica,1997, 21(3-:197-205[陈敏容, 杨兴淇, 俞小牧, 等. 白鲫(红鲫(` -异源四倍体鱼的倍性操作及其生殖力的研究. 水生生物学报,1997, 21(3):197-205]
    [7]
    Liu S J,Liu Y, Zhou G J, et al.The formation of tetraploid stocks of red crucian carp common carp hybrids as an effect of interspecific hybridization [J]. Aquaculture, 2001, 192: 171-186
    [8]
    Sun Y D,Liu S J, Zhang C, et al.The chromosome number and gonadal structure of F9-F11allotetraploid crucian -carp [J]. Acta Genetica Sinica, 2003,30(5）: 414-418[孙远东, 刘少军, 张纯, 等. 异源四倍体鲫鲤 F9-F11染色体和性腺观察. 遗传学报, 2003, 30(5）:414-418]
    [9]
    Liu S J, Sun Y D, Zhang C, et al. Production of gynogenetic progeny from allotetraploid hybrids of red crucian carp @ common carp [J].Aquaculture, 2004,236: 193-200
    [10]
    Williams J G, Kubelik A R, Livak K J, et al. DNA Polymorphisms amplified by arbitrary primers are useful as genetic markers [J].NucleicAcids Res., 1990,18: 6531-6535
    [11]
    Welsh J,Mc ClellandM.Fingerprinting genomes using PCRwith arbtrary primers [J]. Nucleic Acids Res,1990,18: 7213-7218
    [12]
    Zhou L,Fan L C, Gui J F. RAPD Analysis of incorporation of heterologous genetic materials in multiple species of silver crucian carp [J].Acta Hydrobiologica Sinica, 1998, 22(4）: 301-306[周莉, 樊连春,桂建芳. 银鲫复合种外源遗传物质整入的分析. 水生生物学报, 1998, 22(4）:301-306]
    [13]
    Yao J H, Lou Y D. Preliminary report on the RAPD analysis of three populations of Carassius auratus gibelio[J]. Journal of Shanghai Fish University,2000, 9(1-: 11-14[姚纪花, 楼允东. 三种群银鲫的RAPD 分析. 上海水产大学学报, 2000, 9(1): 11-14]
    [14]
    Xia D Q, Cao Y,Yang H, et al.The relationships between F1hybrids from tilapia and their parents and the use of theirheterosis [J]. Journal of Fishery Sciences of China, 1999, 6(4): 29-32[夏德全, 曹萤, 杨弘, 等. 罗非鱼杂交F1代与亲本的遗传关系及其杂种优势的利用. 中国水产科学, 1999, 6(4):29-32]
    [15]
    Long L Q, Zhao Z S,Tang B G, et al Analysis of genetic variation in hybrids between Misgurnus anguillicandatus cross Paramisgurnus dabryanus reciprocally by using RAPD [J]. Acta Hydrobiologica Sinica,2000, 24(6):659-662[龙良启, 赵振山, 汤保贵, 等. 泥鳅与大鳞副泥鳅正反交子代遗传变异的 RAPD 分析. 水生生物学报,2000, 24(6):659-662]
    [16]
    Teng C B, Sun X W, Shen J B, et al. RAPD analysis of gynogeneic crucian carp activated by heterologous sperm and their parents[J].Journal of Fisheries of China, 1999, 23(4-: 420-423[藤春波, 孙效文,沈俊宝, 等. 利用异源精子激发雌核发育的银鲫及其亲本的RAPD 分析. 水产学报, 1999, 23 (4): 420-423]
    [17]
    Zhang S M, Deng H,Wang D Q, et al. Population structure and genetic diversity of silver carp and grass carp from population of Yangtze River system revealed by RAPD [J]. Acta Hydrobiologica Sinica,2001, 25(4): 324-330[张四明, 邓怀, 汪登强, 等. 长江水系鲢和草鱼遗传结构及变异性的研究[J]. 水生生物学报, 2001, 25(4): 324-330]
    [18]
    Sun X W, Liang L Q. A genetic linkage map of common carp [J].Journal of Fishery Sciences of China, 2000, 7(1):1-5[孙效文,梁利群,鲤鱼的连锁遗传图谱(初报).中国水产科学,2000,7(1):1-5]
    [19]
    Liang L Q, Sun X W,Yan X C. RAPD analysis of the genomic changein red common carp [J]. Journal of Fishery Sciences of China, 1998,5(1): 6-9[梁利群, 孙效文, 闫学春. RAPD 技术分析荷包红鲤抗寒品系与亲本的基因组变化. 中国水产科学, 1998, 5(1):6-9]
    [20]
    Zhou L, Gui J F.Studies on genetic diversity in gonochoristic offspring produced frommating betweentwo different gynogeneticclones of silver crucian carp [J]. Acta Biologiae Experimentalis Sinica,2001, 34(3）:169-176[周莉, 桂建芳. 银鲫两个雌核发育克隆间两性生子代的遗传多样性分析. 实验生物学报, 2001, 34(3): 169-176]
    [21]
    Sambrook J, Fritsch E F, Maniatis T. Molecular cloning: A libratory manual ed[M]. New York: Cold Spring Harbor Laboratory. 1989,463-468
    [22]
    Lynch M.The similarity index and DNA fingerprinting [J] Mol. Biol.Evo.,1990, 7:478-484
    [23]
    Apostol BL, Black ? W C, Reiter P, et al. population genetics with RAPD -PCRmarkers: the breeding structure of Aedes aegypti in Puerto Rico [J].Heredity, 1996, 76: 325-334
    [24]
    Streisinger G, Walker C, Dower N, et al. Production of clones of homozygous diploid zebra fish(Brachydanio rerio-[J]. Nature, 1981,291 5813: 293-296
    [25]
    Wu Q J, Gui J F. Fish genetics and breeding engineering [M]. Shanghai: Scientific and Technology Publisher. 1999[吴清江, 桂建芳. 鱼类遗传育种工程. 上海: 上海科学技术出版社, 1999]
    [26]
    Zhang H Y, Liu R Z, Zhang X W, et al.Assessment of population genetic variation of grass carp and common carp using RAPD fingerprints [J]. ActaHydrobiologicaSinica, 1998, 22(2-: 168-173[章怀云, 刘荣宗, 张大明, 等. 草鱼和鲤鱼群体遗传变异的 RAPD 指纹分析. 水生生物学报, 1998,22(2): 168-173]
    [27]
    Tajima F.Evolutionary relationship of DNA sequence in finite populations [J]. Genetics, 1983, 105: 437-460
    [28]
    Jia H B, Zhou L, Gui J F. RAPD marker analysis of two artificial gynogenetic populations in red white ornamental carp [J]. Acta Hydrobiologica Sinica, 2002, 26(1-: 1-7[贾海波, 周莉, 桂建芳. 两个人工雌核发育红白锦鲤群体的RAPD 分析. 水生生物学报,2002,26(1-:1-7]
    [29]
    Mao H,Zhou L,Gui J F. RAPD marker analysis of two artificial gynogenetic families in SilverCarp [J].ActaHydrobiologica Sinica, 2003,27(5-: 1-7[毛晗, 周莉, 桂建芳, 鲢两个人工雌核发育家系的RAPD 分析. 水生生物学报, 2003, 27(5):1-7]
    [30]
    Zou G W, Pan G B, Wang D Q, et al. Genetic diversity of artificialgynogenesis silver carp and RAPD analysis of incorporation of hetero-logous genetic matetials in gynogenetic progeny [J]. ActaHydrobiologica Sinica, 2004,28(2-: 180-185[邹桂华, 潘光碧, 汪登强, 等.人工雌核发育鲢的遗传多样性及异源遗传物质整入的 RAPD分析. 水生生物学报, 2004,28(2): 180-185]
    [31]
    Zhou L, Wang Y, Gui J F. Molecular analysis of silver crucian carp (Carassius auratus gibelio Bloch-clones by SCAR markers [J].Aquaculture, 2001, 201: 219-228
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