STUDIES ON PHOTOSYNTHETIC PROPERTIES OF DUNALIELLA SALINA CELLS

Photosynthetic propenies of Dunaliella salina cells were inveshgated.The following results were obtained: (1) The absorption spectra of D.salina cells in the range of 700-300nm exhibited three high peaks at 680, 485 and 440nm.(2) The value of ATP / ADP in samples of D.salina cells was measured.The inhibitor of plasma membrane-Sodium vanadate increased the value of ATP/ADP in the cells.(3) Photosynthetic O2-evolution of D.salina cells was measured when the culture fluids were used as reaction medium.lt was found that sodium vanadate delayed the induction, inhibited the rate of photosynthetic O2-evolution.(4) The F0, Fm and Fm of chlorophyll a in D.salina cells were measured by pluse modulation fluorometry.It showed that the abilities of reoxidation of PSⅡ in the cells were normal.(5) Reversible light state transition in the cells were induced by lightdark.DCMU and NaF inhibited the reversion of Cells in state 2 to in state 1.(6) The preparations of thylakoid membranes of control and treatment cells of D.salina by chloromycetin were isolated.Different stan densities of proteins in the control and treatment samples were showed at the pattem of SDS- PAGE.