WU Hou-Bo, PAN Jin-Pei. EFFECTS OF CULTURAL CONDITIONS ON THE PRODUCTION OF TOXIN BY VIBRIO MIMICUS[J]. ACTA HYDROBIOLOGICA SINICA, 2003, 27(2): 119-121.
Citation: WU Hou-Bo, PAN Jin-Pei. EFFECTS OF CULTURAL CONDITIONS ON THE PRODUCTION OF TOXIN BY VIBRIO MIMICUS[J]. ACTA HYDROBIOLOGICA SINICA, 2003, 27(2): 119-121.

EFFECTS OF CULTURAL CONDITIONS ON THE PRODUCTION OF TOXIN BY VIBRIO MIMICUS

  • Received Date: July 09, 2001
  • Rev Recd Date: December 28, 2001
  • Published Date: March 24, 2003
  • In this article, effect of cultural conditions such as temperature, pH, air conditions, culture medium and incubation time on the yield of toxin Vm-Pm by Vibrio mimicus were studied. Temperature had significant influence on the toxin Vm-Pm production, the optimal temperature for toxin Vm-Pm production was 30℃, and the optimal initial pH for the toxin Vm-Pm production was pH 6—8. The yield of toxin Vm-Pm was significantly enhanced when growing the strain with shaking. The yield of toxin Vm-Pm was the maximal during the mid- and late-logarithmic growth phases (when the strain was incubated for 36 hours), and was stable during the stationary growth phase(incubated for 72 hours). The yield of toxin in the improved medium containing 0.1% Fe2+ was twice more than that in the ordinary marine water agar, the result demonstrated that the availability of free iron in the medium had a significant influence on the production of toxin Vm-Pm,and also the pathogenesis of Vibrio mimicus.
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    Wu H B, Pan J P. The immune protective effects of three kinds of vaccination against vibriosis in maring-cage cultured red sea bream(pagrus major)[J]. Acta Hydrobiol. Sinica, 2002, 26(5):457-464[吴后波,潘金培. 三种免疫制剂对真鲷弧菌病的免疫保护性,水生生物学报,2002,26(5):457-464][2] Wu H B, Pan J P. Purification and characterization of the exotoxin Vm-Pm produced by vibrio mimicus [J]. Oceanologia et Limnologia sinica, 33(1): 83-89[吴后波,潘金培. 海水养殖真鲷弧菌病病原菌外毒素的分离、提纯及生物学活性. 海洋与湖沼,2002,33(1):83-89][3] Kothary M H, Kreger A S. Production and partial characterization of an elastolytic protease of Vibrio vulnificus[J]. Infect. Immun., 1985,50:534-540[4] Kothary M H,Kreger A S. Purification and characterization of an extracellular cytolysin produced by Vibrio damsela[J].Infect. Immun., 1985, 49: 25-31[5] Kothary M H, Kreger A S. Production and characterization of an elastolytic protease of Vibrio vulnificus [J]. Journal of General Microbiology, 1987, 133: 1783-1791[6] Tison D L, Kelly M T. Factors affecting hemolysin production by Vibrio vulnificus[J]. Curr. Microbiol., 1984, 10:181-184[7] Kreger A S. Cytolytic activity and virulence of Vibrio damsela[J]. Infect. Immun., 1984, 44: 326-331[8] Nishibuchi M, Seidler R J. Medium-dependent production of extracellular enterotoxin by non-01 Vibrio cholerae, Vibrio mimicus, and Vibrio fluvialis[J]. Appl.Environ. Microbiol., 1983, 45: 228-231[9] Amaro C, Gbiosca E, Fouz B, et al. Role of iron, capsule, and toxins in the pathogenicity of Vibrio vulnificus biotype 2 for mice[J]. Infect. Immun., 1994, 62: 759-763

    Wu H B, Pan J P. The immune protective effects of three kinds of vaccination against vibriosis in maring-cage cultured red sea bream(pagrus major)[J]. Acta Hydrobiol. Sinica, 2002, 26(5):457-464[吴后波,潘金培. 三种免疫制剂对真鲷弧菌病的免疫保护性,水生生物学报,2002,26(5):457-464][2] Wu H B, Pan J P. Purification and characterization of the exotoxin Vm-Pm produced by vibrio mimicus [J]. Oceanologia et Limnologia sinica, 33(1): 83-89[吴后波,潘金培. 海水养殖真鲷弧菌病病原菌外毒素的分离、提纯及生物学活性. 海洋与湖沼,2002,33(1):83-89][3] Kothary M H, Kreger A S. Production and partial characterization of an elastolytic protease of Vibrio vulnificus[J]. Infect. Immun., 1985,50:534-540[4] Kothary M H,Kreger A S. Purification and characterization of an extracellular cytolysin produced by Vibrio damsela[J].Infect. Immun., 1985, 49: 25-31[5] Kothary M H, Kreger A S. Production and characterization of an elastolytic protease of Vibrio vulnificus [J]. Journal of General Microbiology, 1987, 133: 1783-1791[6] Tison D L, Kelly M T. Factors affecting hemolysin production by Vibrio vulnificus[J]. Curr. Microbiol., 1984, 10:181-184[7] Kreger A S. Cytolytic activity and virulence of Vibrio damsela[J]. Infect. Immun., 1984, 44: 326-331[8] Nishibuchi M, Seidler R J. Medium-dependent production of extracellular enterotoxin by non-01 Vibrio cholerae, Vibrio mimicus, and Vibrio fluvialis[J]. Appl.Environ. Microbiol., 1983, 45: 228-231[9] Amaro C, Gbiosca E, Fouz B, et al. Role of iron, capsule, and toxins in the pathogenicity of Vibrio vulnificus biotype 2 for mice[J]. Infect. Immun., 1994, 62: 759-763

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