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LIU Zheng-Hua, CHEN Jin-Hui, HUANG Ming-Min, ZHENG Kang, LUO Chen. GENETIC ANALYSIS ON SOME RAPD LOCI FOR IDENTIFICATION OF MOLECULAR MARKERS IN GRASS CARP GENOMIC DNA[J]. ACTA HYDROBIOLOGICA SINICA, 2006, 30(3): 292-297.
Citation: LIU Zheng-Hua, CHEN Jin-Hui, HUANG Ming-Min, ZHENG Kang, LUO Chen. GENETIC ANALYSIS ON SOME RAPD LOCI FOR IDENTIFICATION OF MOLECULAR MARKERS IN GRASS CARP GENOMIC DNA[J]. ACTA HYDROBIOLOGICA SINICA, 2006, 30(3): 292-297.
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GENETIC ANALYSIS ON SOME RAPD LOCI FOR IDENTIFICATION OF MOLECULAR MARKERS IN GRASS CARP GENOMIC DNA

  • Institute of Biology, Hunan Normal University, Changsha 410081

  • Received Date: July 13, 2005
  • Rev Recd Date: January 19, 2006
  • Published Date: May 24, 2006
    Graphical Abstract
    • Abstract
  • The products amplified by Random Amplified Polymorphism DNA(RAPD) techniques are always variable in different experiments. In order to establish a universal RAPD molecular marker system for grass carp genomic DNA polymorphism analysis,selected polymorphism RAPD loci should be checked by using different strains with distinct genetic backgrounds according to the law of Mendelian genetics. In this experiment, some polymorphic RAPD loci that could be employed as molecular markers for grass carp genomic DNA analysis have been examined by comparatively analyzing the genomes of theXiangjiang River grass carp group(Xiangjiang group) and a two-generation artificial induced meio-gynogenetic grass carp group(meio-gynogenetic-2 group). The Xiangjiang group can provide distinct genetic backgrounds samples andmay include all the genotype of grass carp,while the meiogynogenetic-2 group can provide clear genetic background samples. The different characterizations of the two groups can help identify the polymorphic RAPD loci in grass carp. Total 36 individuals of the Xiangjiang group and total 29 individuals of meiogynogenetic-2 group were examined with 10 polymorphism random primers in this experiment.With RAPD-PCR method, total 30 polymorphic loci have been identified in Xiangjiang group. In meiogynogenetic- 2 group, 17 of the polymorphic loci identified in Xiangjiang group were not detected in all the examined individuals. Only 13 of the 30 polymorphic loci in Xiangjiang group were identified in meio-gynogenetic-2 group.However only 7 of the 13 detected loci showed polymorphism, the other 6 lociwere identified in all the individuals in meio-gynogenetic-2 group and no polymorphism was observed. Because the meio-gynogenetic-2 individuals are highly purified during the gynogenesis and artificial selection for two generations, obviously, the 17 polymorphic loci that were undetected in meio-gynogenetic-2 group but in Xiangjiang group and the 6 polymorphic loci that detected in all the examined individuals of the meio-gynogenetic-2 group are the real polymorphic loci in grass carp.A chi-squared test was done on the 7 loci that show polymorphism to determine if the ratio of the detected and undetected individualswere consistent with the 1B1 expected ratio. The P values of the 7 polymorphic loci are from 0. 1936 to 0. 5775. This genetic statistical analysis indicated that the 7 polymorphic loci in Xiangjiang group and meio- gynogenetic-2 group are also consistent with the law of Mendelian genetics well. The 30 polymorphic RAPD loci, therefore, could be used as reliable molecular markers for the grass carp genomic DNA analysis. The observat ion that 17 polymorphic loci have been lost during the process of the two-generation artificial gynogenesis strongly suggested that protection of the diversity of natural grass carp resource and selection of homozygous traits with desired economic genotypes are both very important for grass carp breeding.
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    • References (20)
  • [1]
    Williams J G K, Kubel ik A R. DNA polymorphisms amplified by arbitrary primer are useful as genetic markers[J].Nucleic Acids Research, 1990, 18: 6531-6535
    [2]
    Welsh J, McClelland M. Fingerprinting genomes using PCR with arbitrary primers[J].Nucl eic Acids Research, 1990, 18: 7213-7218
    [3]
    Carl W. Diffenbath and Gabriela S. Dveksler(Translated by Huang Peitang, Yu Weiyuan, Chen Tianmi et al.).PCR Primer: A Laboratory Manual[M]. Beijing:Science Press, 2000[ C.W. 迪芬巴赫,G. S. 德维克斯勒著( 黄培堂, 俞炜源, 陈添弥, 等译). PCR 技术实验指南. 北京: 科学出版社, 2000]
    [4]
    Wu C J, Gui J F. Fish Genet ics and Breeding Engineering[ M]. Shanghai: Shanghai Scientific & Technical Publ ishes, 1999[ 吴清江,桂建芳. 鱼类遗传育种工程. 上海: 上海科学技术出版社,1999]
    [5]
    Zou S M, Li S F, Cai W Q et al. Establishing gynogenetic groups of genetic improvedMegalobrama amblycephala and its genetic analysis[J].Journal of Fisheries of China, 2001, 25( 4): 311-316[ 邹署明, 李思发, 蔡完其, 等. 团头鲂良种雌核发育群体的建立及其遗传变异. 水产学报, 2001, 25(4) 311-316]
    [6]
    Lu R Rank G H. Use of RAPD population genetics parameters in the alfalfa leaf cutting bee, Megachile rotundata[J].Genome, 1996, 39:63- 70
    [7]
    Xia D Q, Cao Y, Wu T Tet al. Study on lineages ofProtosalanx chinensis, Neosalanx taihuensis andN. oligodontis in Taihu Lake with RAPD technique[J].Journal of Fisheries of China, 2000,7(1):12-15[ 夏德全, 曹莹, 吴婷婷, 等. 用RAPD 方法分析太湖大银鱼、太湖新银鱼和寡齿新银鱼的亲缘关系. 中国水产科学, 2000, 7(1): 12- 15]
    [8]
    Lin J Z, RitlandK. Construction of a genetic linkage map in the wild plant Mimulus using RAPD and isozyme markers[J].Genome,1996,39:63-70
    [9]
    Iturra P, Medrano J F, Bagley Met al. Identification of sex chromosome molecular markers using RAPDs and fluorescent in situ hybridization in rainbow trout[ J].Gentica,1998,101:209-213
    [10]
    Zhou Li, Fan L C, Gui J Fet al. RAPD analysis of incorporation of heterologousgenetic materials in multiple species of Silver Crucian carp[J].Acta Hydrobiol ogica Sinica, 22(4): 301-303[ 周莉, 樊连春, 桂建芳, 等. 银鲫复合种外源遗传物质整入的RAPD 分析.水生生物学报, 1998, 22(4): 301- 303]
    [11]
    Zhang S M, Deng H, Wang D Q et al. Population structure and diversity of silver carp and grass carp from populat ion of Yangtze River system revealed by RAPD[J].Acta Hydrobi ologica Sinica, 2001, 25(4): 324-330 [ 张四明, 邓怀, 汪登强, 等. 长江水系鲢和草鱼遗传结构及变异性的RAPD 指纹分析. 水生生物学报,2001, 25(4):324-330]
    [12]
    Xue G X, Liu J. RAPD analysis of grass carp population in three- river water[J].Journal of Fishery Sciences of China, 1998, 5(1):1-5[ 薛国雄, 刘棘. 三江水系草鱼种群RAPD 分析. 中国水产科学, 1998, 5(1):1-5]
    [13]
    Chen J H, Huang M M. RAPD analysis on genomic DNA of two artificial gynogenetic groups of grass carp,Ctenopharyngodon idellus[J].ActaHydrobiologica Sinic, 2004,28(5):471-477[ 陈金辉,黄明敏. 两个不同雌核发育群体基因组DNA 的RAPD 分析.水生生物学报, 2004, 28(5):471-477]
    [14]
    Huang M M, Chen J H. Screening of polymorphic primers and polymorphic locus for grass carp genomic RAPD analysis,Cenopharyngodon idellus[J].Science Technology and Engineering, 2004, 4(2):91-96[ 黄明敏, 陈金辉. 草鱼基因组随机扩增多态性引物及多态性位点筛选. 科学技术与工程,2004,4(2):91-96]
    [15]
    Liu C L, Guan C Y. Studies on the authent icity of plant marker[ J].Biotechnology Inf ormation, 1999, 2: 31- 34[ 刘春林, 官成云. 植物RAPD 标记的可靠性研究. 生物技术通报, 1999, 2: 31-34]
    [16]
    Luo C, Liu Y. Studies on production of gynogenetic grass carp and crucian carp[ J].Acta Sci. Univ. Norm. Hunan, 1991, 14(2):154- 159[ 罗琛, 刘筠. 人工诱导草鱼和鲫鱼雌核发育的研究.湖南师范大学自然科学学报, 1991, 14(2):154-159]
    [17]
    St anley J G. Morphology of androgent ic and gynogenetic grass carp,Ctenopharyngodon idella(Valenciennes).J. Fish Boli, 1976, 9:523-528
    [18]
    Li B X, Luo C. Cyt ologi cal observations on induction mitogynogenesis by heat shocking of the eggs in grass carp,Ctenopharyngodon idellus[J].ActaHydrobiologica Sinica, 2003, 27(2): 155- 160[ 李冰霞, 罗琛. 热休克法抑制第一次卵裂实现草鱼雌核发育的细胞学观察. 水生生物学报, 2003, 27(2): 155-160]
    [19]
    Damato, M. E., D. Corach. Genetic diversity of populations of the fresh shrimpMacrobrachium borelliievaluated by RAPD analysis[J].Journal of crustacean Biology, 1996,16(4): 578-588
    [20]
    Wang J, Quan C G. RAPD analysis of the reared and wildPseudosciaena crocea[J].Acta Oceanologica Sinica, 2001, 23(3):87-91[ 王军, 全成干. 宫井大黄鱼遗传多样性的RAPD 分析. 海洋学报,2001, 23(3):87-91]
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