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Lin Huimin, He Zhenrong, Du Daixian, Dai Lingfen, Xing Wusen, Li Shanghao. STUDIES ON NITROGENASE MO-FE PROTEIN OF BLUE-GREEN ALGAE[J]. ACTA HYDROBIOLOGICA SINICA, 1986, 10(1): 15-23.
Citation: Lin Huimin, He Zhenrong, Du Daixian, Dai Lingfen, Xing Wusen, Li Shanghao. STUDIES ON NITROGENASE MO-FE PROTEIN OF BLUE-GREEN ALGAE[J]. ACTA HYDROBIOLOGICA SINICA, 1986, 10(1): 15-23.
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STUDIES ON NITROGENASE MO-FE PROTEIN OF BLUE-GREEN ALGAE

  • Institute of Hydrobiology, Academia Sinica, Wuhan

  • Received Date: January 20, 1985
  • Published Date: January 24, 1986
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    • Abstract
  • Methods of separation and purification of nitrogenase of blue-green algae were improved. With anaerobic preparative polyacrylamide gel electrophoresis instead of usual chromatogrophy, Mo-Fe protein was purified to electrophoresis purity. The procedure was simplified and the duration of process was shortened.Gel filtration of the Mo-Fe protein resulted a molecular weight of 360,000. SDS electrophoresis showed that it was compoed of 4 identical subunits each having a molecular weight of 90,000. The amino acid, residues/mole Mo-Fe protein were 3290. Acidic amino acids were predominant. The Km for acetylene was found to be 3.33×10-3 atmospheres. The isoelectric point was 5—5.5. UV/visible absorption spectrum of Mo-Fe protein was similar to that of Mo-Fe protein from bacterial source. Salt inhibited nitrogenase from blue-green algae more strongly than that from bacteria.
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