LI Xuan, QIAO Chuan ling, XU Ying. STUDIES ON THE ESTERASE POLYMORPHISM OF THE CHIRONOMUS COMPLEX POPULATION IN THE YA-ER LAKE NEAR WUHAN[J]. ACTA HYDROBIOLOGICA SINICA, 2002, 26(2): 148-154.
Citation: LI Xuan, QIAO Chuan ling, XU Ying. STUDIES ON THE ESTERASE POLYMORPHISM OF THE CHIRONOMUS COMPLEX POPULATION IN THE YA-ER LAKE NEAR WUHAN[J]. ACTA HYDROBIOLOGICA SINICA, 2002, 26(2): 148-154.

STUDIES ON THE ESTERASE POLYMORPHISM OF THE CHIRONOMUS COMPLEX POPULATION IN THE YA-ER LAKE NEAR WUHAN

  • Received Date: March 22, 2000
  • Rev Recd Date: April 19, 2000
  • Published Date: March 24, 2002
  • This paper dealed with the high resistant larvae of Chironomus complex which were collected from the Ya Er Lake near Wuhan. The results indicated that high active esterases existed in the population, the Rf is difference from conference population of Culex pipiens. The data of the GPD from Chironomus complex in four ponds indicated that the pattern of the electrophoresis is difference from the populations of Culex pipiens quinquefasciatus mosquitoes. In the middle polluted 2#, 3# pond, the GPD locus of the larvae of Chironomus complex demonstrated polymorphisms. The analysis results by GENEPOP software showed that the hypothesis of Hardy Weinberg equilibrium was rejected. There was systemic heterozygote deficit in these sub populations, no significant differentiation was found among the three sub populations 2#, 3#,4#, the gene flow among them was low according to the number of migrants of per generation(Nm).
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    Xu Y, Wu W Z.Mobility and transfer of residual Hexachlorocyclohexane(HCH)in aquatic environment [J]. Fresenius Envir. Bull, 1994, 3: 557-562[2] 翟启惠.昆虫分子生物学的一些进展:杀虫剂抗性的分子基础[J].昆虫学报,1995,38:493-500[3] Hemingway J, S H P P Karunaratne. Mosquito carboxylesterases: a review of the molecular biology and biochemistry of a major insecticide resistance mechaniam [J]. Med. Vet. Entomol., 1998, 12: 1-12[4] Herath P R, Hemingway J. The detection and characterization of malathion resistance in filed population of Anopeles Culicifacies B in Sri Lanka Pestic [J]. Biochem. Physiol., 1987, 29: 157-162[5] Bisset J A, Rodriguez M M, Hemingway J, et al. Malathion and pyrethroid resistance in Culex quinquefasciatus from Cuba: efficacy pf primiphos-methyl in the presence of at laese three resistance mechanisms [J]. Med. Vet. Entomol., 1991, 5: 223-228[6] Karunaratne S H P P, Jayawardena K G I, Hemingway J, et al. Characterization of a B-type esterase involved in insecticide resistance from the mosquito Culex quinquefasciatus [J]. Biochem J, 1993, 294: 575-579[7] Raymond M, Qiao C L, Callaghan A, et al. Easterase polymorphism in insecticide susceptible populations of the mosquito Culex pipiens [J]. Genet Res Camb, 1996, 67: 19-26[8] Ranasinghe L E, Georghiou G P. Comparative modification of insecticide resistance spectrum of Culex fatigans Wied by selection with temephos and temephos/synergist combinations [J]. Pestic Science, 1979, 10: 502-508[9] Georghiou G P, Pasteur N, Hawley M K, et al. Linkage relationships between organophosphate resistance and a highly active esterase B in Culex pipiens quinquefasciatus Say from California [J]. J Econ Entomol, 1980, 73: 301-305[10] Wirth M C, Marquine M, Geoghiou G P, et al. Esterase A2 and B2 in Culex quinquefasciatus(Diptera: Culicidae): Role in organophoaphate resistance and linkage studies [J]. Med Entomol, 1990, 27: 202-206[11] Pasteur N, Pasteur G, Catalan J, et al. Practical Isozyme Genetics [M]. Ellis Hrwood Lmd, Chister, England. 1988[12] Haldane J B C. An exact test for randomness of mating [J]. J Genet, 1954, 2: 631-635[13] Louis E J, Dempster E R. An exact test for Hardy-Weinberg and multiple alleles [J]. Biometrics, 1987, 43: 805-811[14] Guo S W, Thompson E A. Performing the exact test of Hardy-Weinberg proportions for multiple alleles [J]. Biometrics, 1992, 48: 361-372[15] Weir B S, Cockerham C C. Estimating F-statistics for the analysis of population stucture [J]. Evolution, 1984, 38:1358-1370[16] Fisher R A. Statistical methods for research workers(14th Edit)[M]. Edinbourgh: Olivier and Boyd. 1970[17] Weir B S, Cockerham C C. Estimating F-statistics for the analysis of population stucture [J]. Evolution, 1984, 38: 1358-1370[18] Wright S. Evolution and genetics of populations Vol.2. 1969. The theory of gene frequencies [M]. Chicago: Chicago University Press.[19] Nei M. Molecular evolutionary genetics [M]. New York: Columbia Univ press. 1987

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