THE CROSS -SPECIES AMPLIFICATION AND VALIDATION OF EST -SSR LOCI IN PORPHYRA HAITANENSIS

LIU Bi-Qian; ZENG Qing-Guo; WANG Ya-Jun; LUO Qi-Jun

Volume 31 Issue 2

Aug. 2014

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ACTA HYDROBIOLOGICA SINICA > 2007 > 31(2): 149-154.

LIU Bi-Qian, ZENG Qing-Guo, WANG Ya-Jun, LUO Qi-Jun. THE CROSS -SPECIES AMPLIFICATION AND VALIDATION OF EST -SSR LOCI IN PORPHYRA HAITANENSIS[J]. ACTA HYDROBIOLOGICA SINICA, 2007, 31(2): 149-154.

Citation:

LIU Bi-Qian, ZENG Qing-Guo, WANG Ya-Jun, LUO Qi-Jun. THE CROSS -SPECIES AMPLIFICATION AND VALIDATION OF EST -SSR LOCI IN PORPHYRA HAITANENSIS[J]. ACTA HYDROBIOLOGICA SINICA, 2007, 31(2): 149-154.

Citation:

LIU Bi-Qian, ZENG Qing-Guo, WANG Ya-Jun, LUO Qi-Jun. THE CROSS -SPECIES AMPLIFICATION AND VALIDATION OF EST -SSR LOCI IN PORPHYRA HAITANENSIS[J]. ACTA HYDROBIOLOGICA SINICA, 2007, 31(2): 149-154.

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THE CROSS -SPECIES AMPLIFICATION AND VALIDATION OF EST -SSR LOCI IN PORPHYRA HAITANENSIS

The marine biotechnology key lab of Ningbo University, Ningbo 315211;
2. The fishery institute of Ningbo, Ningbo 315100

Funds:

National natural science foundation No.49976030

Received Date: June 08, 2005
Rev Recd Date: November 05, 2006
Published Date: March 24, 2007

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Abstract

Abstract

Multiple banding pattern, a potential problem of cross-species microsatellite amplilification, happened when EST-SSR primers of Porphyra yezoensis were used in microsatellite amplification for several lines of Porphyra haitanensis. These PCR products were sequenced to identify false positive amplification. Among the recovered productsfrom P. haitanensis., bands with simlar size to the amplification product of positive control from P. yezoensis contained the anticipated SSR. Sequence alignment showed high identity. The microsatellite signature was absent from the amplification products with bigger molecular weight. This result showed a typical false positive problem. Therefore primersthat producedfalse positive products in P. haitanensis can not be used in those genetic analyses based onmodels of SSR mutation, such as the diploid conchocelia pure line test of P. haitanensis.However, they are still useful for the species identification and other genetic researches.
- Microsatellite,
- SSR,
- False positive,
- Multiple banding pattern

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References

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