Volume 34 Issue 2
Aug.  2014
Turn off MathJax
Article Contents
Abstract
References
LI Yang, HE Li-Na, MA Yan-Yan, L�Song-Hui. THE STUDY ON MORPHOLOGICAL TAXONOMY OF PSEDUO-NITZSCHIA PSEUDODELICATISSIMA COMPLEX[J]. ACTA HYDROBIOLOGICA SINICA, 2010, 34(2): 302-311.
Citation: LI Yang, HE Li-Na, MA Yan-Yan, L�Song-Hui. THE STUDY ON MORPHOLOGICAL TAXONOMY OF PSEDUO-NITZSCHIA PSEUDODELICATISSIMA COMPLEX[J]. ACTA HYDROBIOLOGICA SINICA, 2010, 34(2): 302-311.
shu

THE STUDY ON MORPHOLOGICAL TAXONOMY OF PSEDUO-NITZSCHIA PSEUDODELICATISSIMA COMPLEX

  • Guangdong Provincial Key Laboratory of Healthy and Safe Aquaculture, Key Laboratory of Ecology and Environmental Science in Guangdong Higher Education, College of Life Science, South China Normal University, Guangzhou 510631, China;
    2. Institute of Harmful Algae and Aquatic Environment, College of Science and Engineering, Jinan University, Guangzhou 510632, China

  • Received Date: October 08, 2008
  • Rev Recd Date: September 24, 2009
  • Published Date: March 24, 2010
    Graphical Abstract
    • Abstract
  • Increasing evidence show that cryptic and pseudo-cryptic species are widespread in several diatom taxa. This is the case with the genus Pseudo-nitzschia, which includes chain-forming pennate diatoms that bloom both in coastal and open ocean waters. In the last years, up 10 new species have been described on the basis of morphological and molecular investigations, and some of them are only distinguishable on the basis of minute ultrastructural features of their siliceous frustulae. This is the case of P. pseudodelicatissima complex. It has been confirmed that P. pseudodelicatissima complex was a kind of pelagic diatom species which distribute commonly in Chinese coastal waters. Because of few subtle morphological features which just could be observed under electronic microscope (EM) among P. pseudodelicatissima complex taxa, it is greatly difficult to reach accurate identification and there are many debates at present. For clarifying the species diversity of P. pseudodelicatissima complex and its dominant species in Daya Bay, water and net samples were collected from six stations in summer (August), 2007. All samples were fixed with Lugo’s solution in situ and brought back to the laboratory. Then, they were concentrated to a final volume about 2—10 mL by settlement overnight for several times. These samples could be observed and analyzed under light microscope. For observation under transmission electron microscope, subsamples were taken and treated through several steps. Firstly, after dropped into same volume of concentrated H2SO4, these samples were boiled in the water bath for about 15—20 minutes to remove the organic materials. Secondly, these samples should be washed to neutrality with distilled water for several times. Then, these samples could be observed, identified, photographed and counted under transmission electron microscope. Including six species, P. caciantha Lundholm, Moestrup & Hasle, P. calliantha Lundholm, Moestrup & Hasle, P. cuspidata (Hasle) Lundholm, Moestrup & Hasle, P. mannii Amato & Montresor, P. pseudodelicatissima (Hasle) Lundholm, Hasle & Moestrup and P. sinica Qi & Wang, P. pseudodelicatissima complex were found. And P. caciantha, P. calliantha, P. mannii were identified as three newly recorded diatom species for China. Along with LM and TEM photos, the detailed morphological description, habits and ecological distribution of these six species were given in this paper. Simultaneously, the taxonomic comparison among resembling species were made. The six species described in this paper have morphological features in common: the long and slender valves, the present of a larger central interspace, the uniseriate striae, the similarity of the structure of the valve face and the valve mantle, and the hexagonal perforation pattern of the poroid hymen. However, several other features such as structure of the poroid hymen, density of fibulae and striae, the width and shape of valves, and the structure of the cingular bands differed among the species and could be used for differentiating these six species from each other. After investigation of the cell abundance of each species, it is concluded that the most common species distributing in Daya Bay is P. calliantha, not P. pseudodelicatissima which has been reported formerly.
    • FullText(HTML)
    • References (27)
  • [1]
    Bates S S, Bird C J, Freitas D E. Pennate diatom, Nitzschiapungens, as the primary source of domoic acid, a toxin inshellfish form eastern Prince Edward Island, Canada [J].Canadian Journal Fishery Aquiculture Science, 1989, 46:1203—1215.
    [2]
    Bates S S, Garrison D L & Homer R A. Bloom dunamics andphysiology of domoic acid-producing Pseudo-nitzschia species[A]. In Anderson D M, Cembella A D, Hallegraeff G M(Eds.), Physiological ecology of harmful algal blooms [M].Heidelberg: Springer-Verlag. 1998, 267—292.
    [3]
    Hallegraeff G M. Species of the diatom genus Pseudonitzschiain Australian waters [J]. Botanica Marina, 1994,37: 397—411308 水生生物学报 34 卷.
    [4]
    Chen J F, Xu N, Wang Z H, et al. Dynamics ofPseudo-nitzschia spp. and environmental factors in Daya Bay,the South China Sea [J]. Acta Scientiae Circumstantiae, 2002,22(6): 743—748 [陈菊芳, 徐宁, 王朝辉, 等. 大亚湾拟菱形藻种群的季节变化与环境因子的关系. 环境科学学报,2002, 22(6): 743—748].
    [5]
    Gao Y, Liang J R, Gao Y H, et al. Review of morphologicaland molecular identification of Pseudo-nitzschia, a bloomforming diatom [J]. Marine Sciences, 2005, 29(1): 67—72[高杨, 梁君荣, 高亚辉, 等. 赤潮拟菱形藻形态学分类和分子生物技术鉴定研究概述.海洋科学, 2005, 29(1): 67—72].
    [6]
    Hasle G R. Pseudo-nitzschia as a genus distinct fromNitzschia (Bacillariophyceae) [J]. Journal of Phycology,1994, 30: 1036—1039.
    [7]
    Amato A & Montresor M. Morphological, phylogeny, andsexual cycle of Pseudo-nitzschia mannii sp. nov. (Bacillariophyceae):a pseudo-cryptic species within the P. pseudodelicatissimacomplex [J]. Phycologia, 2008, 47(5): 487—497.
    [8]
    Qi Y Z, Wang J, Zheng L. The taxonomy and bloom ecologyof Pseudo-nitzschia on the coasts of China [A]. In ProceedingsIOC-WESRPAC Third International Scientific Symposium[M]. Bali: Indonesia Institute of Sciences. 1994, 88—95.
    [9]
    Chen J F, Qi Y Z, Xu N, et al. Pseudo-nitzschia bloom andits ecological role in the biological community in the DayaBay, the South China Sea [J]. Acta Oceanologica Sinica,2005, 27(1): 114—119 [陈菊芳, 齐雨藻, 徐宁, 等. 大亚湾拟菱形藻水华及其在生物群落中的生态地位. 海洋学报, 2005, 27(1): 114—119].
    [10]
    Chen J F, Qi Y Z, Xu N, et al. Annual dynamics of phytoplanktoncommunity and abundance at Aotou Cover, DayaBay [J]. Acta Hydrobiologica Sinica, 2006, 30(3): 311—317 [陈菊芳, 齐雨藻, 徐宁, 等. 大亚湾澳头水域浮游植物群落结构及周年数量动态. 水生生物学报, 2006, 30(3):311—317].
    [11]
    Gao Y H, Yu Q B, Qi Y Z, et al. Species composition andecological distribution of planktonic diatoms in the ChangjiangRiver estuary during Spring [J]. Chinese Journal of AppliedEcology, 2003, 14(7): 1044—1048 [高亚辉, 虞秋波,齐雨藻, 等. 长江口附近海域春季浮游硅藻的种类组成和生态分布. 应用生态学报, 2003, 14(7): 1044—1048].
    [12]
    Gao Y H, Xie W L, Li Y, et al. Pseudo-nitzschia: taxonomyand biogeographical distribution in the estuary of ChangjiangRiver, China [A]. In Witkowski A. (Eds.), Proceedings of18th International Diatom Symposium [M]. Bristol: BiopressLimited. 2006, 75—90.
    [13]
    Lundholm N, Moestrop ?, Hasle G R, et al. A study of thePseuso-nitzschia pseudodelicatissima/cuspidate complex(Bacillariophyceae): what is P. pseudodelicatissima [J]?Journal of Phycology, 2003, 39: 797—813.
    [14]
    Martin J L, Haya K, Burridge L E, et al. Nitzschia pseudodelicatissima—a source of domoic acid in the Bay of Fundy,eastern Canada [J]. Marine Ecological Progress Series, 1990,67: 177—182.
    [15]
    Lundholm N, Skov J, Pocklington R, et al. Studies of themarine planktonic diatom Pseudo-nitzschia 2. Autecology ofP. pseudodelicatissima based on isolates from Danish coastalwaters [J]. Phycologia, 1997, 36: 381—388.
    [16]
    Lundholm N, Skov J, Pocklington R, et al. Domoic acid, thetoxic amino acid responsible for amnesic shellfish poisoning,now in Pseudo-nitzschia seriata (Bacillariophyceae) inEurope [J]. Phycologia, 1994, 33: 475—478.
    [17]
    Skov J, Lundholm N, Moestrop ?, et al. Potentially toxicphytoplankton 4. The diatom genus Pseudo-nitzschia (Diatomophyceae/Bacillariophyceae) [A]. In Lindley J A (Eds.),ICES identification leaflets for plankton ICES [M]. Copenhagen:Palaegade 2-4, DK-1261. 1999, 1—23.
    [18]
    Buri? Z, Vili?i? D, Mihali? K C, et al. Pseudo-nitzschiablooms in the Zrmanja River Estuary (Estuary Adriatic Sea)[J]. Diatom Research, 2008, 23(1): 51—63.
    [19]
    Hasle G R, Lange C B, Syvertsen E E. A review ofPseudo-nitzschia, with special reference to the Skagerrak,North Atlantic and adjacent waters [J]. Helgol Meeresunters,1996, 50: 131—175.
    [20]
    Xing X L, Yang J X, Kang Y Y, et al. Morphological studyand toxic analysis for two Pseudo-nitzschia species isolatedfrom Daya Bay [J]. Journal of Oceanography in TaiwanStrait, 2007, 26(4): 576—582 [邢小丽, 杨军霞, 康燕玉,等. 大亚湾水域两种拟菱形藻的形态学鉴定及毒素分析.台湾海峡, 2007, 26(4): 576—582].
    [21]
    Mann D G, McDonald S M, Bayer M M, et al. The Sellaphorapupula species complex (Bacillariophyceae): morphometricanalysis, ultrastructure and mating data provideevidence for five new species [J]. Phycologia, 2004, 43:459—482.
    [22]
    Sarno D, Kooistra W H C F, Medlin L K, et al. Diversity inthe genus Skeletonema (Bacillariophyceae) Ⅱ. An assessmentof the taxonomy of S. costatum-like species, with thedescription of four new species [J]. Journal of Phycology,2005, 41: 151—176.
    [23]
    Amato A, Kooistra W H C F, Levialdi G J H, et al. Reproductiveisolation among sympatric cryptic species in marinediatoms [J]. Protist, 2007, 158: 193—207.
    [24]
    Beszteri B, John U, Medlin L K. An assessment of crypticgenetic diversity within the Cyclotella meneghiniana speciescomplex (Bacillariophyta) based on nuclear and plastidgenes, and amplified fragment length polymorphisms [J].European Journal of Phycology, 2007, 42: 47—60.
    [25]
    Vanormelingen P, Chepurnov V A, Mann D G, et al. Geneticdivergence and reproductive barriers among morphologicallyheterogeneous sympatric clones of Eunotia bilunaris senuslato (Bacillariophyta) [J]. Protist, 2008, 159: 73—90.
    [26]
    Mann D G, Evans K M. Molecular genetics and the neglectedart of diatomics [A]. In Brodie J & Lewis J M (Eds), Unravellingthe algae- the past, present and future of algal molecularsystematics [M]. London: CRC Press. 2007, 231—265.
    [27]
    Kaczmarska I, Fryxell G.A. The diatom genus Nitzschia:morphologic variation of some small bicapitata species intwo Gulf Stream Warm Core Rings [A]. In Ricard M (Eds.),Proceeding of the eighth international diatom symposium[M]. Koenigstein: Otto Koeltz Science Publishers. 1986,237—252.
    • Related Articles

Catalog

    Get Citation
    PDF
    XML
    Article views (1056) PDF downloads (705) Cited by()
    Related

    Editorial Office of Acta Hydrobiologica Sinica

    Address：No. 7 Donghu South Road, Wuchang District, Wuhan, Hubei Province, China

    Tel：027-68780701；027-68780800 E-mail：acta@ihb.ac.cn  

    Supported byBeijing Renhe Information Technology Co. Ltd  Technical support：info@rhhz.net

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return