HUANG Feng, YAN An-Sheng, ZHANG Gui-Rong, ZOU Gui-Wei. EFFECT OF DIFFERENT DIETARY PROTEIN CONTENT ON ACTIVITIES OF PEPSIN AND AMYLASE OF SILURUS MERIDIONALIS CHEN[J]. ACTA HYDROBIOLOGICA SINICA, 2003, 27(5): 451-456.
Citation: HUANG Feng, YAN An-Sheng, ZHANG Gui-Rong, ZOU Gui-Wei. EFFECT OF DIFFERENT DIETARY PROTEIN CONTENT ON ACTIVITIES OF PEPSIN AND AMYLASE OF SILURUS MERIDIONALIS CHEN[J]. ACTA HYDROBIOLOGICA SINICA, 2003, 27(5): 451-456.

EFFECT OF DIFFERENT DIETARY PROTEIN CONTENT ON ACTIVITIES OF PEPSIN AND AMYLASE OF SILURUS MERIDIONALIS CHEN

  • Received Date: October 25, 2002
  • Rev Recd Date: December 21, 2002
  • Published Date: September 24, 2003
  • In order to research the relationship between the nutrients or feedstuffs and the digestive enzymes in fish,it was studied in this paper that the changes of pepsin or acid protease and amylase activities in pancreas,stomach,foregut and hindgut of Silurus meridionalis were measured at 0d,15d,25d and 50d with different protein content diet,containing protein 44.8%,29.1%and 13.3%and naming high protein content diet(HPCD),intermediate protem content diet(IPCD)and low protein content diet (LPCD) respectively.The results are as follows: 1) Silurus meridionalis in HPCG and IPCD groups (44.8% and 29.1%) grow faster than does in LPCD group;In the anaphase (after 25d),the growth rate of this fish in IPCD group is biggest.It shows that the protein content in pellets is about 29.1% or little more,but not more than 45%. 2) During the former experiment (before 25d),the pepsin or acid protease activities in stomach show the tendency to decrease in all three groups.But in the later experiment(after 25d),the pepsin activity in stomach of S.meridionalis in the IPCD group tends to increase,while that to decrease in other two groups.These show that the protein content in diets can influence the pepsin activities in stomach of the fish.The acid protease activities in pancreas,foregut and hindgut are very low,they had very little action on digesting diet.Combined to the growth of this fish,it shows that there have some correlations between the changes of pepsin activities in stomach and growth after a period time of rearing. 3) In the whole experiment,the amylase activities in pancreas are higher than that in other three organs or tissues in the three groups.And in the earlier experiment(before 15d),the amylase activities in pancreas decreased slowly,but increased rapidly from 15d to 25d.Then this enzyme activity became stable after 25d in the HPCD group,but in other two groups the activities kept to increase. 4) During the whole the amylase activities fluctuated to some extent in all groups,but in the later period(after 25d),the amylase activities in the foregut and hindgut in the LPCD group decrease rapidly,but this enzyme activities still increase in the same time.These also suggest that amylase enzyme activities or their changes correlate to the growth of this species. 5) Over the whole experiment,the characters of the distribution of pepsin or acid protease and amylase activities to all tissues or organs studied are similar in all three groups. It shows that the protein content in diets did not influence the distribution of these two enzyme activities to all tissues or organs. The changes of the two enzyme activities among the three groups in the experiment suggest that how much protein in diets influences the synthesis,secretion and action of pepsin in stomach and amylase of this species.
  • [1]
    Baragi V,Lovell R T. Digestive enzyme activities in striped bass from first feeding through larva development [J]Trans.Am.Fish.Soc,1986,115:478-484[2] Cahu CL,Zambonino Infante JLZ.Effect of the molecular form of dietary nitrogen supply on sea bass larvae:Response of pancreatic enzymes and intestinal peptidases. [J]Fish.physiol.Biochem 1995,14(3):209-214[3] Pedersen BH, Ugelstad I,Hjelmeland K.Effects of a transitory,low food supply in the early life of larval herring (Clupea harengus)on mortaliy, growth and digestive capacity [J]. Mar.Biol.1990,107(1):61-66[4] Kawai S,Ikeda S.Studies on the digestive enzymes of fishes.Ⅱ.Effect of dietary change in the activities of digestive enzymes in carp intestine [J]. Bull.Jpn.Soc.Sci.Fish.1972,38:265-270[5] National Research Council.Nutrient Requirement of Fish [M].Washingtong, National Academy Press. 1993[6] Huang F,Yan A,Wang X. Studies on trypsin in silver carp and bighead carp [J]. Journal of Fisheries of China,1996,20(1):68-71.[黄峰、严安生、汪小东等,鲢、鳙胰蛋白酶的研究.水产学报,1996,20(1):68-71][7] Huang F,Yan A S,Zhang G R, et al. The protease and amylase of Hypophthalmichthy molitrix and Aristichthys nobilis [J]. Journal of Fishery Science of China,1999,6(2):14-17.[黄峰、严安生,张桂蓉,等,鲢、鳙蛋白酶和淀粉酶的研究.中国水产科学,1999,6(2):14-17][8] Jiang CK. Activity Measuring for Implemental Enzyme [M]. Shanghai:Science and Technology Press,1982.[蒋传葵. 工具酶的活力测定. 上海科学技术出版社,1982][9] Li A J.Feed and Nutriology of Aquatic Animal [M]. Beijing:Agricultural Press,1996.[李爱杰,水产动物营养与饲料学.北京:中国农业出版社,1996][10] Rosch R,Senger H.Development of dry food for larvae of Coregonus lacaretus L.Ⅰ. Growth, food digestion and fat absorption [J]. Aquaculture,1990,91:101-115[11] Tian LX,Lin D Changes in digestive enzyme activities in grass carp Ctenopharyngodon idellus after feeding of two test diets [J]. Acta Hydrobiologia Sinica,1993,17(1):58-65[田丽霞、林鼎,草鱼摄食两种蛋白质饲料后消化酶活性变动比较.水生生物学报,1993,17(1):58-65][12] Munilla-Moran R,Stark J R. Protein digestion in early turbot larvae,Scophthalmus maximus(L.) [J]. Aquaculture.1989,81(3-4):315-327[13] Pedersen B H,Nilssen E M,Hjelmeland K.Variations in the content of trypsin and trypsinogen in larval herring(Clupea harengus)digesting copepod nauplii [J]. Mar.Biol.1987.94(2):171-181[14] Ragyanszki M.Preliminary investigations on the proteolytic digestive enzymes of carp fry [J]. Aquacult. Jung.(Szarvas).1980,2:27-30.(abstract)[15] Hjelmeland K.Pedersen BH,Nilssen EM.Trypsin content in intestines of herring larvae,Clupea harengus,ingesting inert polystyrene spheres or live Crustacea prey. [J]. Mar.Biol.1988,98(3):331-335[16] Gawlicka A,McLaughlin L,Hung S S O,de-la-Noue J. Limitations of carrageenan microbound diets for feeding white sturgeon,Acipenser transmontanus,lavvae [J]. Aquaculture 1996,141(3-4):245-265[17] Dabrowski K,Glogowski J. Studies on the proreplytic enzymes of invertebrates constituting fish food [J]. Hydrobiologia.1977a,52:171-174[18] Abi-Ayad A,Kestemont P.Comparison of the nutritional status of goldfish(Carassius auratus)larvae fed with live,mixed or dry diet [J]. Aquaculture 1994,128(1-2):163-176[19] Caruso G, Genovese L,Greco S.Effect of two diets on the enzymatic activity of Pagellus acarne(Brunnich 1768)in intensive rearing [J]. From-Discovery-To-Commercialization. Carrillo,-M.; Dahle,-L.; Morales,-J.; Sorgeloos,-P.; Svennevig,-N.; Wyban,-J.-Eds. Oostende-Belgium European-Aquaculture-Soc.1993,19:332[20] Lemaire P, Drai P, Mathieu A,Lemaire S,Carriere S,Giudicelli J,Lafaurie M. Changes with different diets in plasma enzymes(GOT,GPT,LDH,ALP)and plasma lipids (cholesterol,triglycerides) of sea-bass(Dicentrarchus labrax) [J]. Aquaculture. 1991,93(1):63-75

    Baragi V,Lovell R T. Digestive enzyme activities in striped bass from first feeding through larva development [J]Trans.Am.Fish.Soc,1986,115:478-484[2] Cahu CL,Zambonino Infante JLZ.Effect of the molecular form of dietary nitrogen supply on sea bass larvae:Response of pancreatic enzymes and intestinal peptidases. [J]Fish.physiol.Biochem 1995,14(3):209-214[3] Pedersen BH, Ugelstad I,Hjelmeland K.Effects of a transitory,low food supply in the early life of larval herring (Clupea harengus)on mortaliy, growth and digestive capacity [J]. Mar.Biol.1990,107(1):61-66[4] Kawai S,Ikeda S.Studies on the digestive enzymes of fishes.Ⅱ.Effect of dietary change in the activities of digestive enzymes in carp intestine [J]. Bull.Jpn.Soc.Sci.Fish.1972,38:265-270[5] National Research Council.Nutrient Requirement of Fish [M].Washingtong, National Academy Press. 1993[6] Huang F,Yan A,Wang X. Studies on trypsin in silver carp and bighead carp [J]. Journal of Fisheries of China,1996,20(1):68-71.[黄峰、严安生、汪小东等,鲢、鳙胰蛋白酶的研究.水产学报,1996,20(1):68-71][7] Huang F,Yan A S,Zhang G R, et al. The protease and amylase of Hypophthalmichthy molitrix and Aristichthys nobilis [J]. Journal of Fishery Science of China,1999,6(2):14-17.[黄峰、严安生,张桂蓉,等,鲢、鳙蛋白酶和淀粉酶的研究.中国水产科学,1999,6(2):14-17][8] Jiang CK. Activity Measuring for Implemental Enzyme [M]. Shanghai:Science and Technology Press,1982.[蒋传葵. 工具酶的活力测定. 上海科学技术出版社,1982][9] Li A J.Feed and Nutriology of Aquatic Animal [M]. Beijing:Agricultural Press,1996.[李爱杰,水产动物营养与饲料学.北京:中国农业出版社,1996][10] Rosch R,Senger H.Development of dry food for larvae of Coregonus lacaretus L.Ⅰ. Growth, food digestion and fat absorption [J]. Aquaculture,1990,91:101-115[11] Tian LX,Lin D Changes in digestive enzyme activities in grass carp Ctenopharyngodon idellus after feeding of two test diets [J]. Acta Hydrobiologia Sinica,1993,17(1):58-65[田丽霞、林鼎,草鱼摄食两种蛋白质饲料后消化酶活性变动比较.水生生物学报,1993,17(1):58-65][12] Munilla-Moran R,Stark J R. Protein digestion in early turbot larvae,Scophthalmus maximus(L.) [J]. Aquaculture.1989,81(3-4):315-327[13] Pedersen B H,Nilssen E M,Hjelmeland K.Variations in the content of trypsin and trypsinogen in larval herring(Clupea harengus)digesting copepod nauplii [J]. Mar.Biol.1987.94(2):171-181[14] Ragyanszki M.Preliminary investigations on the proteolytic digestive enzymes of carp fry [J]. Aquacult. Jung.(Szarvas).1980,2:27-30.(abstract)[15] Hjelmeland K.Pedersen BH,Nilssen EM.Trypsin content in intestines of herring larvae,Clupea harengus,ingesting inert polystyrene spheres or live Crustacea prey. [J]. Mar.Biol.1988,98(3):331-335[16] Gawlicka A,McLaughlin L,Hung S S O,de-la-Noue J. Limitations of carrageenan microbound diets for feeding white sturgeon,Acipenser transmontanus,lavvae [J]. Aquaculture 1996,141(3-4):245-265[17] Dabrowski K,Glogowski J. Studies on the proreplytic enzymes of invertebrates constituting fish food [J]. Hydrobiologia.1977a,52:171-174[18] Abi-Ayad A,Kestemont P.Comparison of the nutritional status of goldfish(Carassius auratus)larvae fed with live,mixed or dry diet [J]. Aquaculture 1994,128(1-2):163-176[19] Caruso G, Genovese L,Greco S.Effect of two diets on the enzymatic activity of Pagellus acarne(Brunnich 1768)in intensive rearing [J]. From-Discovery-To-Commercialization. Carrillo,-M.; Dahle,-L.; Morales,-J.; Sorgeloos,-P.; Svennevig,-N.; Wyban,-J.-Eds. Oostende-Belgium European-Aquaculture-Soc.1993,19:332[20] Lemaire P, Drai P, Mathieu A,Lemaire S,Carriere S,Giudicelli J,Lafaurie M. Changes with different diets in plasma enzymes(GOT,GPT,LDH,ALP)and plasma lipids (cholesterol,triglycerides) of sea-bass(Dicentrarchus labrax) [J]. Aquaculture. 1991,93(1):63-75
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