GENETIC D IVERSITY OF CRUC IAN CARP (CARASSIUS AURATUS) FROM Y IL I　RIVER OF SINKIANG D ISTRICT

Studies about genetic diversity of crucian carp (Carassius auratus) have been executed formany years. However, the samples usually come from the area of Heilong River in northeast, Yangtze River and Yellow River in the middle part of China, rarely from the district of SinkiangDistrict in northwest of China. This study aim at accumulating evidences for realizing genetic diversity and finding some characteristic marks of genetic diversity of crucian carp in the district of Sinkiang District in northwest of China. The sampled crucian carps (Carassius auratus) captured with townet from Yili River of Sinkiang district. Their sex was identified according to the gland at the anatomical level. DNA content of blood corpuscle was mensurated with Phoenix Flow Systems after blood corpuscle was dyed with propidium iodide (PI) solution. Transferrin (Tf) in blood serum was measured with non-denatualization polyacrylamide gel electrophoresis. Itwas staimed with coommassie brilliant blue G22501 The result indicates that there are both ofmale and female among the crucian carpgroups. DNA content of blood corpuscle of crucian carp is about 1153 times of the color diploid crucian carp (actual 5.315 ±0.215pg/nucleus: 3.475 ±0.079 pg/nucleus, respectively). The quantity is matching with that of the gibel carp (Carassius auratus gibelio Bloch). Nine phenotype of Tf have been discovered among those crucian carps, controlled by seven alleles1 Its genic frequency is Tfa0.063、Tfb0.063、Tff0.095、Tfg0.169、Tfc0.174、Tfe0.175 and Tfd0.270. Genotype frequency is Tfde0.13, Tfcd0.043, Tfce0.13, Tfdf0.13, Tfcdg0.26, Tfcdf 0.043, Tfdef0.087, Tfabeg0.13, Tfabdg0.043, respectively, presenting a very plentiful genetic diversity1 These indicate that there are two kinds of reproductive modes of sexual reproduction and clonal reproduction of gynogenesis among this group of Crucian Carp (Carassius auratus) from Yili River of Sinkiang District. The crucian carps (Carassius auratus) are silver Crucian carp (Carassius auratus　gibelio Bloch). Diversity in reproductive modesmay play an important role in ecological adaptation of crucian carps. They　may have evolved over several undiscovered event in history.