Volume 33 Issue 4
Aug.  2014
Turn off MathJax
Article Contents
Abstract
References
ZHOU Qiu-Bai, ZHENG Yu, ZHOU Li, GUI Jian-Fang. GENETIC D IVERSITY OF CRUC IAN CARP (CARASSIUS AURATUS) FROM Y IL I　RIVER OF SINKIANG D ISTRICT[J]. ACTA HYDROBIOLOGICA SINICA, 2009, 33(4): 690-695.
Citation: ZHOU Qiu-Bai, ZHENG Yu, ZHOU Li, GUI Jian-Fang. GENETIC D IVERSITY OF CRUC IAN CARP (CARASSIUS AURATUS) FROM Y IL I　RIVER OF SINKIANG D ISTRICT[J]. ACTA HYDROBIOLOGICA SINICA, 2009, 33(4): 690-695.
shu

GENETIC D IVERSITY OF CRUC IAN CARP (CARASSIUS AURATUS) FROM Y IL I　RIVER OF SINKIANG D ISTRICT

  • Department of Animal Science and Technology, Jiangxi Agricultural University, Nanchang330045 China;
    2. State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan430072 China

  • Received Date: November 09, 2008
  • Rev Recd Date: April 18, 2009
  • Published Date: July 24, 2009
    Graphical Abstract
    • Abstract
  • Studies about genetic diversity of crucian carp (Carassius auratus) have been executed formany years. However, the samples usually come from the area of Heilong River in northeast, Yangtze River and Yellow River in the middle part of China, rarely from the district of SinkiangDistrict in northwest of China. This study aim at accumulating evidences for realizing genetic diversity and finding some characteristic marks of genetic diversity of crucian carp in the district of Sinkiang District in northwest of China. The sampled crucian carps (Carassius auratus) captured with townet from Yili River of Sinkiang district. Their sex was identified according to the gland at the anatomical level. DNA content of blood corpuscle was mensurated with Phoenix Flow Systems after blood corpuscle was dyed with propidium iodide (PI) solution. Transferrin (Tf) in blood serum was measured with non-denatualization polyacrylamide gel electrophoresis. Itwas staimed with coommassie brilliant blue G22501 The result indicates that there are both ofmale and female among the crucian carpgroups. DNA content of blood corpuscle of crucian carp is about 1153 times of the color diploid crucian carp (actual 5.315 ±0.215pg/nucleus: 3.475 ±0.079 pg/nucleus, respectively). The quantity is matching with that of the gibel carp (Carassius auratus gibelio Bloch). Nine phenotype of Tf have been discovered among those crucian carps, controlled by seven alleles1 Its genic frequency is Tfa0.063、Tfb0.063、Tff0.095、Tfg0.169、Tfc0.174、Tfe0.175 and Tfd0.270. Genotype frequency is Tfde0.13, Tfcd0.043, Tfce0.13, Tfdf0.13, Tfcdg0.26, Tfcdf 0.043, Tfdef0.087, Tfabeg0.13, Tfabdg0.043, respectively, presenting a very plentiful genetic diversity1 These indicate that there are two kinds of reproductive modes of sexual reproduction and clonal reproduction of gynogenesis among this group of Crucian Carp (Carassius auratus) from Yili River of Sinkiang District. The crucian carps (Carassius auratus) are silver Crucian carp (Carassius auratus　gibelio Bloch). Diversity in reproductive modesmay play an important role in ecological adaptation of crucian carps. They　may have evolved over several undiscovered event in history.
    • FullText(HTML)
    • References (26)
  • [1]
    Wu XW. The Cypriniformes fish ofChina(partⅡ) [M]. Shanghai: Shanghai Science and Technology Press. 1982, 430-435[伍献文. 中国鲤科鱼类志, 下卷. 上海: 上海科技出版社. 1982, 430-435]
    [2]
    Shen J B. The ploidy of crucian carp and its geographical distribution in heilongjiangmain river [J]. Journal of Fisheries of China, 1983, 7 (2) : 87-91 [沈俊宝. 黑龙江主要水域鲫鱼倍性及其地理分布. 水产学报, 1983, 7 (2) : 87-91]
    [3]
    Yang L. Genetic diversity among silver crucian carp. Carasius auratus gibelo revealed by transferrin and its molecular markers [D]. Thesis for Doctor of Science. Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan. 2000 [杨林. 银鲫转铁蛋白及其相关分子标记的遗传多样性研究. 博士学位论文.中国科学院水生生物研究所, 武汉. 2000]
    [4]
    Shen J B, Fan Z T, Wang G R. Karyotype Studies of Male Triploid Crucian Carp (Fangzheng Crucian Carp) in Heilongjiang [J]. Journal of Genetics and Genom ics, 1983, 10 (2) : 133-136 [沈俊宝, 范兆廷, 王国瑞. 黑龙江一种银鲫 (方正银鲫)群体三倍体雄鱼的核型研究. 遗传学报, 1983, 10 (2) :133-136]
    [5]
    Zhu L F, Jiang Y G. Intraspecific genetic markers of crucian carp (Carassius auratus gibelio) and their application to selective breeding [J]. Acta Hydrobiologica Sinica, 1987, 12 (2) : 105-111 [朱蓝菲, 蒋一珪. 银鲫种内的遗传标记及其在选种中的作用. 水生生物学报, 1987, 12 (2) : 105-111]
    [6]
    Zhou L, Wang Y, Gui J F. Genetic evidence for gonochoristic reproduction in gynogenetic silver crucian carp (Carassius auratus gibelio Bloch) as revealed by RAPD assays [J]. J. M o1. Evol.,2000, 51: 498-506
    [7]
    Gui J F, Zhou L F, Liang S C, et al. Genetic evidences for gynogenetic reproductive mode in embryogenesis of the multiple tetraploid crucian carp (Carassius auratus gibelio Bloch) [J]. Chinese ScienceBulletin, 1992, 37 (9) : 836-838 [桂建芳, 朱蓝菲, 梁绍昌, 等. 人工复合四倍体异育银鲫雌核发育生殖方式的初步证明. 科学通报, 1992, 37 (9) : 836-838]
    [8]
    Gui J F, Liang S C, Zhu L F, et al. Discovery of two different reproductive developmentmodes of the eggs of artificial multiple tetraploid allogynogenetic silver crucian carp [J]. Chinese Science Bull 1993, 38 (4) : 332-337
    [9]
    Zhou L, Gui J F. Studies on genetic diversity in gonochoristic off spring produced from mating between two different gynogenetic clones of silver crucian carp [J]. Acta B iologiae Experimentalis Sinica, 2001, 34 (3) : 169-176 [周莉, 桂建芳. 两个银鲫克隆间两性生殖子代的遗传多样性分析. 实验生物学报,2001, 34 (3) : 169-176]
    [10]
    Zhou L, Gui J F. Karytypic diversity in polyploid gibel carp, Carassius auratus gobelio Bloch [J]. Genetica, 2002, 115:223-232
    [11]
    YangL, Yang S T, WeiX H, et al. Genetic diversity among different clones of the gynogeneic silver crucian carp Carasius auratus gibelo revealed by transferrin and isozyme markers [J]. B iochem ical Genetics, 2001, 39: 213-225
    [12]
    HerschbergerW K. Some physicochemical properties of transferrins in brook trout [J]. Trans. Amer. Fish.Soc., 1970, 1:207-218
    [13]
    Verspoor E. Spatial correlations of transferrin allele frequencies in atlantic salmon (Salmo salar) populations from North America[J]. Can J Fish. Aquat. Sci, 1986, 43: 1074-1078
    [14]
    Yang L, Gui J F. Genetic analysis on diversity of reproductive modes of silver crucian carp inferred by transferrin and isozyme markers [J]. Acta B iologiae Experimentalis Sinica, 2002, 35(4) : 263-269 [杨林, 桂建芳. 银鲫生殖方式多样性的转铁蛋白和同功酶标记的遗传分析. 实验生态学报, 2002, 35 (4) : 263-269]
    [15]
    Long H, Zeng Y, Li G. Present status of studies on fish serum transferrin and perspective of its application [J]. Journal of Fisheries of China, 2001, 25 (2) : 181-186 [龙华, 曾勇, 李谷. 鱼类血清转铁蛋白的研究现状与应用前景. 水产学报, 2001, 25 (2) : 181-186]
    [16]
    Zhang X Z, Qiu Q R, Chen Z L. Fish genetics and selective breeding [M].Beijing: Agriculture Press.1988, 108-109[张兴忠, 仇潜如, 陈曾龙. 鱼类遗传与育种. 北京: 农业出版社. 1988, 108-109]
    [17]
    Jiang Y G, Liang S C, Chen B D, et al. Biological effect of heterologous sperm on gynogenetic offspring in Carassius auratus gibelio [J]. Acta Hydrobiologica Sinica, 1983, 8 (1) : 1-13[蒋一珪, 梁绍昌, 陈本德, 等. 异源精子在银鲫雌核发育子代中的生物学效应. 水生生物学学报, 1983, 8 (1) : 1-13]
    [18]
    Fan Z T, Han Y, Ma X Z. Studies on the sperm biology of crucian carp (Carassius auratus gibelio Bloch) in Heilongjiangwaters [J]. Journal of Northeast Agricultural University, 2003, 34 (4) : 414-418 [范兆廷, 韩英, 马旭洲. 黑龙江银鲫 (Carassius auratus gibelio Bloch)精子生物学研究. 东北农业大学学报, 2003, 34 (4) : 414-418]
    [19]
    Li YC. Comparative investigation of the genetic diversity between C. auratus gibelio (Bloch) and C. auratus auratus (L.) [D]. Thesis forMaster of Science, Northeast Agricultural University, Heilongjiang. 2001 [李因传. 黑龙江银鲫与普通鲫鱼遗传多样性比较研究. 硕士学位论文, 东北农业大学, 黑龙江.2001]
    [20]
    LiuM H, Shen J B. A histological study on hermaphroditism of crucian carp [J]. Journal of Fishery Sciences of China, 1998, 5 (2) : 6-7 [刘明华, 沈俊宝. 黑龙江银鲫雌雄同体的组织学. 中国水产科学, 1998, 5 (2) : 6-7]
    [21]
    LiM Y, Zhou L, Yang L, et al. Molecular genetic analysis on Pengze crucian carp and comparison with strain A of gynogenetic silver crucian carp [J]. Journal of Fisheries of China, 2002, 26 (5) : 472-476 [李名友, 周莉, 杨林, 等. 彭泽鲫的分子遗传分析及其与方正银鲫 A系的比较. 水产学报, 2002, 26 (5) : 472-476]
    [22]
    Guo W, Gui J F. Microsatellite marker isolation and cultured strain identification in Carassius auratus gibelio [J]. Aquacult Int., 2009, 16 (6) : 497-510
    [23]
    GuoW. Isolation ofmicrosatellite markers and population genetic analysis in large yellow croaker and gibel carp [D]. Thesis for Doctor of Science. Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan. 2007 [郭葳. 大黄鱼与银鲫微卫星标记的分离及其群体遗传分析. 博士学位论文. 中国科学院水生生物研究所, 武汉. 2007]
    [24]
    Yang L, Gui J F. Positive selection on multiple antique allelic lineages of transferringin the polyploid Carassius auratus [J]. M ol. biol. Evol., 2004, 21: 1264-1277
    [25]
    Lu C Y, Yang Y H, Tong G X, et al. Comparative studiesof genetic diversityBetween C. auratus gibelio (Bloch) and C. auratus auratus (L.) in the same reservoir [J]. Chinese Journal of Fisheries, 2006, 19 (2) : 42-50 [鲁翠云, 杨彦豪, 佟广香, 等.同水体银鲫与普通鲫遗传多样性的比较研究, 水产学杂志,2006, 19 (2) : 42-50]
    [26]
    Li S F. Study on genetic resources of major freshwater fishes of China [M]. Shanghai: Shanghai Science and Technology Press.1998, 111-113 [李思发. 中国淡水主要养殖鱼类种质研究.上海: 上海科技出版社. 1998, 111-113]
    • Related Articles

Catalog

    Get Citation
    PDF
    XML
    Article views PDF downloads Cited by()
    Related

    Editorial Office of Acta Hydrobiologica Sinica

    Address：No. 7 Donghu South Road, Wuchang District, Wuhan, Hubei Province, China

    Tel：027-68780701；027-68780800 E-mail：acta@ihb.ac.cn  

    Supported byBeijing Renhe Information Technology Co. Ltd  Technical support：info@rhhz.net

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return