WU Ying-song, WANG Jian-guo. THE IMMUNOGENICITY OF MYXOBOLUS ROTUNDUS NEMECZEK, 1911[J]. ACTA HYDROBIOLOGICA SINICA, 2000, 24(3): 246-251.
Citation: WU Ying-song, WANG Jian-guo. THE IMMUNOGENICITY OF MYXOBOLUS ROTUNDUS NEMECZEK, 1911[J]. ACTA HYDROBIOLOGICA SINICA, 2000, 24(3): 246-251.

THE IMMUNOGENICITY OF MYXOBOLUS ROTUNDUS NEMECZEK, 1911

  • Received Date: January 12, 1998
  • Rev Recd Date: January 16, 2000
  • Published Date: May 24, 2000
  • An indirect haemagglutination test was empolyed to detect circulating antibodies in the sera of Carassius auratus Linnaeus infected naturally with Wyxobolus rotundus Nemeczek. The circulating antibodies were detected in naturally infected C.auratus. But statistical correlation between infection intensities and antibody levels was found. Polyantibodies were prepared by injecting New Zealand white rabbit with soluble antigen of M. rotundus. It was suggested that same antigens among different life stages of M .rotundus, and the genus-specific antigens be presented by using enzyme-linked immunoassay (ELISA). By using the indirect fluorescent antibody technique (IFAn, fluorescence was found densely concentrated in the rear point and periphery of spores of M. rotundus. However, the two polar capsules were obviously unstained in the fluorescent background. The anti-M. rotundus antiserum could react with M. guanqiaoensis Wu et Wang by IFAT, fluorescence was observed in sporoplasm and the anterior end of the sppre wall. At the same time, polar filaments were fainily fluorescent. Trophozoites of the two parasites could react with anti-M. rotundus antiserum. Fluorescence was scattered on the whole parasites.
  • [1]
    Nemeczek A. Beitrage zur Kenntnis der Myxo-und Microsporidien der Fische[J]. Archiy fur Protistenkunde.,1911, 22:143-169[2] Amandi A. Holt R A. Fryer J L. Observations on myxobolus insidiosus (Myxozoa: Myxosporea) a parasite of salmonid fishes[J]. Fish pathology., 1985, 20:287-304[3] Griffin B R. Davis E M. Myxosoma cerebralis: detection of circulating antibodies in infected rainbow trout (Salmo gairdneri)[J]. J. Fish Res. Board Can., 1978, 35:1186-1190[4] Markiw M E. Wolf K. Myxosoma cerebralis: fluorescent antibody techniques for antigen recognition[J]. J.Fish. Res board. Can., 1978, 35:828-832[5] Furuta T. Ogawa K. Wakabayashi H. Humoral immune response of carp Cyprinus carpio to Myxobolus artus (Myxozoa: Myxobolidae) infection[J]. J. Fish Biology., 1993, 43:441-450[6] Pauley G B. Fish sporozoa: extraction of antigens from Myxosoma cerebralis spores which mimic tissue antigens of raibow trout (Salmo garidneri) [J]. J. Fish Res. Board Can., 1974, 31:1481-1484[7] Halliday M M. Studies on Myxosoma cerebralis, a parasite of salmonids. Ⅳ. A preliminary immunofluorescent investigation of the spores of Myxosoma cerebralis[J]. Nordisk Veterinaer Medicin.,1974, 26:173-179[8] Siua Y. Immunologic observations in fish of the genus Mugil parasitized by the myxospooridan Myxobolus exiguus Thelohan, 1895[J]. Zeitschrift fur parasitenkcunde., 1980., 62:1-6[9] Bartholomew J L. Robovec J S. Fryer J L. Development characterization and use of monoclonal and polyclonal antibodies against the myxosporean, Ceratomyxa shasta[J]. J. Protozoology., 1989a, 36(4):397-401[10] 刘玉斌.动物免疫学实验技术[M].长春:吉林科学出版社,1989,63-67[11] 王世若王兴龙韩文瑜.动物免疫学[M].长春:吉林科学出版社,1996,354-358[12] 吴英松汪建国.寄生异育银鲫的碘泡虫一新种(粘孢子纲:双壳目,碘泡科)[J].水生生物学报,1997,21(3):268--270[13] 谢杏人杨名义肖武汉.鲤白细胞对"未明血液生物体”(UBO)的免疫反应[C].见:鱼病学研究论文集(第一辑),北京:海洋出版社.1993,72-76[14] Halliday M. The biology of Myxosoma cerebralis: the causative organism of whirling disease of salmonids[J].J. Fish Biology., 1976, 9:339-357[15] Kent M L. Hedrick R P., PKX, the causative agent of proliferative kidney disease (PKD) in Pacific salmonid fishes and its affinities with the Myxozoa[J]. J. Protozoology., 1985, 32:254-260[16] Bartholomew J L. Smith C E. Robovec J S. Characterization of a host response to the myxosporean parasite, Ceratomyxa shaste (Nobel), by histology, scanning electron microscopy and immunological techniques[J]. J. Fish Dis., 1989b, 12:509-522[17] Markiw M E. Salmonid whirling disease: myxosporean and actinosporean stages cross-react in direct fluorescent antibody test[J]. J. Fish biology., 1989, 12:137-141

Catalog

    Article views (925) PDF downloads (431) Cited by()
    Related

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return