Volume 30 Issue 3
Aug.  2014
Turn off MathJax
Article Contents
Abstract
References
Related Articles
LIN Xiao-Zhi, LUO Yi-Ping, XIE Xiao-Jun. EFFECTS OF DIETARY CARBOHYDRATE LEVEL ON GLYCOLYTIC ENZYMES AND SERUM GLUCOSE CONCENTRATIONS IN THE JUVENILE SOUTHERN CATFISH AFTER FEEDING[J]. ACTA HYDROBIOLOGICA SINICA, 2006, 30(3): 304-310.
Citation: LIN Xiao-Zhi, LUO Yi-Ping, XIE Xiao-Jun. EFFECTS OF DIETARY CARBOHYDRATE LEVEL ON GLYCOLYTIC ENZYMES AND SERUM GLUCOSE CONCENTRATIONS IN THE JUVENILE SOUTHERN CATFISH AFTER FEEDING[J]. ACTA HYDROBIOLOGICA SINICA, 2006, 30(3): 304-310.
shu

EFFECTS OF DIETARY CARBOHYDRATE LEVEL ON GLYCOLYTIC ENZYMES AND SERUM GLUCOSE CONCENTRATIONS IN THE JUVENILE SOUTHERN CATFISH AFTER FEEDING

  • School of Life Sciences, Southwest-China Normal University, Chongqing 400715

  • Received Date: November 17, 2005
  • Rev Recd Date: February 17, 2006
  • Published Date: May 24, 2006
    Graphical Abstract
    • Abstract
  • Three isoenergetic diets containing 0%,15% and 30% gelatinized corn starch were formulated as control, middle level and high level carbohydrate(CHO) diets, respectively. The juvenile southern catfish,Silurus meridionalis Chen(50.5±0.6g)were acclimated with control diet for 15d at 27.5±0.5℃. Activities of glycolytic key enzymes(HK, GKand PFK21) and the serum glucose concentrations in the southern catfish respectively fed with the three diets were examined, at 3,6,12,24 and 48h after feeding. The results showed that HKactivity in the middle and high CHO groups were significantly higher than that in the control group(P<0.05)at 24h,but no significant difference was found at other time; GK activity was related positively with CHO level, and the absolute measurement of its activity was much lower than that of HK;PFK21 activity was not significantly different among the three treatments at each measuring time. The results suggested that the glucose phosphorylation in the fish is catalyzed mainly by HK, but its activity could not be significantly induced by dietary CHO level; GKactivity was induced by dietary CHO level so that glucose phosphorylation was promoted, but efficiency of the promotion was not more than 30%. In addition, activity of PFK21 which would catalyze fructose-6-phosphate into fructose 1,6-bisphosphate did not change at different CHO levels, therefore both metabolic mechanisms in glycolysis could not significantly accelerate with the serum glucose concentrations increasing, which might explain the hyperglycemia in the carnivorous fish with nutrition condition of high dietary CHO level.Serum glucose concentration in either the middle or the high CHO groups primarily increased gradually to its peak at 12h after feeding, and then decreased to the basic level, which could provided experimental basis for designing blood sampling time in this kind of research.
    • FullText(HTML)
    • References (30)
  • [1]
    Hutchins C G, Rawles S D, Gatlin ⅢDM. Effects of dietary carbohydrate kind and level on growth, body composition and glycemic response of juvenile sunshine bass[J].Aquaculture,1998,161:187-199
    [2]
    Shiau S Y. Utilization of carbohydrates in warmwater fish with particular reference to tilapia,Oreochromis niloticus×O. aureus[J].Aquaculture,1997,151:79-96
    [3]
    Lee S M, Lee J H. Effect of dietary glucose, dextrin and starch on growth and body composition of juvenile starry flounderPlatichthys stellatus[J].Fisheries Science,2004,70:53-58
    [4]
    Page G I, Hayworth KM, Wade R R,et al. Non-specific immunity parameters and the formation of advanced glycosylation end-products(AGE) in rainbow trout,Oncorhynchus mykiss (Walbaum),fed high levels of dietary carbohydrates[J].Aquaculture Research, 1999,30(4):287-297
    [5]
    Nordrum S,Bakke-McKellep A M, Krogdahl A,et al. Effects of soybean meal and salinity on intestinal transport of nutrients in Atlantic salmon(Salmo salar L.) and rainbow trout(Oncorhynchus mykiss)[J].Comparative Biochemistry and Physiology B, 2000,125(3):317-32
    [6]
    Deng D F, Refstie S, Hung S S S. Glycemic and glycosuric responses in white sturgeon(Acipenser transmontnus) after oral administration of simple and complex carbohydrates[J].Aquaculture, 2001,199:107-117
    [7]
    Kumar S, Sahu N P, Pal A K,et al. Effect of dietary carbohydrate on haematology,respiratory burst activity and histological changes in juveniles[J].Fish & Shellish Immunology, 2005, 19:331-344
    [8]
    Wilson R P. Utilization of dietary carbohydrate by fish [J].Aqua-culture,1994,124:67-80
    [9]
    MetónI, Mediavilla D, Caseras A,et al, Effect of diet composition and ration size on key enzyme activities of glycolysis-gluconeogenesis,the pentose phosphate pathway and amino acid metabolism in liver of gilthead sea bream(Sparus aurata)[J].British Journal of Nutrition,1999,82:223-232
    [10]
    Caseras A,Metón I, Fernandez F,et al. Glucokinase gene expression is nutritionally regulated in liver of gilthead sea bream(Sparus aurata)[J].Biochimica et Biophysica Acta,2000,1493:135-141
    [11]
    Panserat S, Médale F, Blin C,et al. Hepatic glucokinase is induced by dietary carbohydrates in rainbow trout, gilthead seabream, and common carp[J].American Journal Physiology Regulatory Integrative Comparative Physiology,2000,278:1164-1170
    [12]
    Panserat S, Capill E, Gutierrez J,et al. Glucokinase is highly induced and glucose-6-phosphatase poorly repressed in liver of rainbow trout(Oncorhynchus mykiss) by a single meal with glucose[J].Comparative Biochemistry and Physiology, Part B,2001,128:275-283
    [13]
    Fu S J,Xie XJ. Nutritional homeostasis in carnivorous southern cat-fish(Silurus meridionalis):is there a mechanismfor increased energy expenditure during carbohydrate overfeeding?[J].Comparative Biochemistry and Physiology, Part A,2004,139:359-363
    [14]
    Luo Y P, Xie X J. Effects of dietary starch on the Serum glucose concentrations in Silurus meridionalis Chen.(wait for express)[罗毅平,谢小军. 饲料碳水化合物对南方鲇血糖水平的影响. 2005,(待发表)]
    [15]
    Fu S J, Xie XJ. Effect of dietary carbohydrate levels on growth performance inSilurus meridionalisChen[J].Acta Hydrobiologica Sinica, 2005,29(3) 82-86[付世建,谢小军. 饲料碳水化合物水平对南方鲇生长的影响. 水生生物学报,2005,29(3):82-86]
    [16]
    Fu S J, Xie X J. The kinetic function of blood glucose in southern catfish fed with diets with different dietary carbohydrate levels.(inpress) [付世建,谢小军. 2005b,南方鲇摄食碳水化合物后的血糖动力学方程(待发表)]
    [17]
    Ackerman P A, Forsyth R B, Mazur C F and Iwama G K. Stress hormones and the cellular stress response in salmonids.Fish Physiology and Biochemistry,2000,23:327-336
    [18]
    Xie XJ, Long T C, Zhang Y G,et al. Reproductive investment in theSilurus meridionalis[J].Journal of Fish Biology, 1998, 53:259-271
    [19]
    Kirchner S, Kaushik S, Panserat S. Effect of partial substitution of dietary protein by a single gluconeogenic dispensable amino acid on hepatic glucose metabolism in rainbow trout(Oncorhynchus mykiss)[J].Comparative Biochemistry and Physiology Part A, 2003, 134:337-347
    [20]
    Bradford M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle dye binding[J].Analytical Biochemistry,1976,72:248-254
    [21]
    Seifter S, Dayton S,Novic B,et al. The estimation of glycogen with the anthrone reagent[J].Arch. Biochem. Biophysi,1950,50:191-200
    [22]
    Werve V d, Lange G, Newgard A,et al. New lessons in the regulation of glucose metabolism taught by the glucose-6-phosphatase system[J].European Journal of Biochemistry, 2000,267:1533-1549
    [23]
    Caseras A, Metón I, Vives C,et al. Nutritional regulation of glucose-6-phosphatase gene expression in liver of the gilthead sea bream(Sparus aurata)[J].British Journal of Nutrition,2002,88:607-614
    [24]
    Whiting S J, Wiggs A J. Effect of nutritional factors and cortisoloin tyrosine aminotransferase activitt in liver of brook trout,Salverinus fontinalis mitchill[J].Comparative Biochemistry and Physiology,1977,58B:189-193
    [25]
    Shimeno S, Takeda M, Takayama S,et al. Adaptation of hepatopan-creatic enzymens to dietary carbohydrate in carp[J].Nippon Suisan Gakkaishi, 1981,47:71-77
    [26]
    Moon T W. Glucose intolerance in teleost fish: fact or fiction[J].Comparative Biochemistry and Physiology Part B,2001,129:243-249
    [27]
    Peres H, Goncalves P, Oliva-Teres A. Glucose tolerance in gilthead seabream(Sparus aurata) and European seabass(Dicentrarchus labrax)[J].Aquaculture,1999,179:415-423
    [28]
    Hemre G I, Mommsen T P, Krogdahl A. Carbohydrates in fish nutrition: effects on growth, glucose metabolism and hepatic enzymes[J].Aquacult. Nutrition,2002,8:175-194
    [29]
    Lin J H, Shiau S Y. Hepatic enzyme adaptation to different dietary carbohydrates in juvenile tilapia(Oreochromix niloticu x. O. aureus)[J].Fish Physiology Biochemistry,1995,14:165-170
    [30]
    Lin S C, Liou C-H, Shiau S-Y. Renal threshold for urinary glucose excretion by tilapia in response to orally administered carbohydrates and injected glucose[J].Fish Physiology and Biochemistr,2000,3:127-132
    • Related Articles

  • Related Articles

    [1]WU Ming-Han, ZHU Bao-Hua, ZHANG Rui-Hao, WANG Lu-Lu, SUN Chang-Ze, PAN Ke-Hou. EFFECTS OF CADMIUM ON THE GROWTH AND EXPRESSION OF UDP-GLUCOSE PYROPHOSPHORYLASE GENE IN PHAEODACTYLUM TRICORNUTUM[J]. ACTA HYDROBIOLOGICA SINICA, 2021, 45(4): 749-755. DOI: 10.7541/2021.2020.132
    [2]XING Shu-Juan, SUN Rui-Jian, MA Jun, WEI Hai-Ming, XU Wei, ZHOU Hui-Hui, ZHANG Yan-Jiao, ZHANG Wen-Bing, MAI Kang-Sen. EFFECTS OF DIETARY CARBOHYDRATE ON GROWTH PERFORMANCE AND GLYCOMETABOLISM OF LARGE YELLOW CROAKER LARIMICHTHYS CROCEA[J]. ACTA HYDROBIOLOGICA SINICA, 2017, 41(2): 265-276. DOI: 10.7541/2017.33
    [3]LI Shu-yun, LIU Hong-yu, TAN Bei-ping, DONG Xiao-hui, YANG Qi-hui, CHI Shu-yan, ZHANG Shuang. Effects of dietary carbohydrate levels on the gene expression and the activity of PEPCK in marine fishes with different food habits[J]. ACTA HYDROBIOLOGICA SINICA, 2015, 39(1): 80-89. DOI: 10.7541/2015.11
    [4]WU Xu-Dong, LIAN Zong-Qiang, HOU Yu-Xia. EFFECTS OF ARSENIC ON ACTIVITIES OF THE METABOLIC ENZYMES, CONTENTS OF RNA AND PROTEIN IN SEVERAL TISSUES OF SILURUS LANZHOUENSIS[J]. ACTA HYDROBIOLOGICA SINICA, 2013, 37(6): 1073-1078. DOI: 10.7541/2013.144
    [5]ZHANG Shi-Liang, AI Qing-Hui, XU Wei, MAI Kang-Sen, LIUFU Zhi-Guo, ZHANG Yan-Jiao. EFFECTS OF DIETARY CARBOHYDRATE TO LIPID RATIO ON GROWTH, FEED UTILIZATION, PLASMA GLUCOSE AND GLYCOLYTIC ENZYME ACTIVITIES OF JUVENILE DARKBARBEL CATFISH, PELTEOBAGRUS VACHELLI[J]. ACTA HYDROBIOLOGICA SINICA, 2012, 36(3): 466-473. DOI: 10.3724/SP.J.1035.2012.00466
    [6]Shahid Mahboob, Bilal Hussain, Zahid Iqbal, Abdul Shakoor Chaudhry. ESTIMATION OF VOLATILE CONSTITUENTS IN THE FISH FLESH FROM WILD AND FARMED CIRRHINA MRIGALA AND CYPRINUS CARPIO[J]. ACTA HYDROBIOLOGICA SINICA, 2009, 33(3): 484-491.
    [7]LIU Bo, XIE Jun, SU Yong-Teng, YU Ju-Hua, TANG Yong-Kai, GE Xian-Ping. EFFECT OF HIGH CARBOHYDRATE LEVELS OF DIETARY ON GROWTH,GK ACTIVITIES AND GK mRNA LEVELS IN TOPMOUTH CULTER (ERYTHROCULTER ILISHAEFORMIS BLEEKER)[J]. ACTA HYDROBIOLOGICA SINICA, 2008, 32(1): 47-53.
    [8]YANG Wei-Dong, ZHANG Xin-Lian, LIU Jie-Sheng, GAO Jie, ZHANG Ping. INHIBITORY EFFECT AND SINKING BEHAVIOUR OF WOOD MEALS FROM CHINA FIR ON ALEXANDRIUM TAMARENSE IN CULTURES[J]. ACTA HYDROBIOLOGICA SINICA, 2005, 29(2): 215-219.
    [9]Wu Jihua, Liang Yanling, Sun Xida. NEWLY RECORDED SPECIES OF FREE-LIVING NEMATODES FROM CHINA (CHROMADORIDA,ENOPLIDA ARAEOLAIMIDA)[J]. ACTA HYDROBIOLOGICA SINICA, 1997, 21(4): 320-321.
    [10]Zhou Dinggang, Wang kangning. EFFECTS OF RESERPINE AND DOMPERIDONE ON LHRH-A INDUCED OVULATION IN MONOPTERUS ALBUS[J]. ACTA HYDROBIOLOGICA SINICA, 1993, 17(1): 98-100.

  • Cited by

    Periodical cited type(7)

    1. 周建强，司思，祁增源，韩银仓，刘秀，孙永刚. 牦牛SMAD1基因第1外显子SNPs检测及与生长性状的关联分析. 西北农业学报. 2023(02): 179-186 .
    2. 米热妮萨·图尔荪托合提，张继虎，郑浪漫，王晨光，刘春洁，刘书东. SMAD1基因在策勒黑羊卵巢不同生理期差异表达及生物信息学分析. 中国畜牧杂志. 2023(10): 185-189 .
    3. 张雨，陈爱华，吴杨平，许艳顺，曹奕，陈素华，张志东. 红壳色文蛤选育群体的经济性状分析. 水产养殖. 2022(05): 1-5 .
    4. 余钧剑，迟美丽，贾永义，刘士力，竺俊全，顾志敏. 雌核发育一代翘嘴鲌抑制素β_B基因SNP筛选及其与生长性状的关联性分析. 江西农业大学学报. 2020(02): 321-330 .
    5. 田志龙，汤继顺，孙庆，王玉琴，张效生，张金龙，储明星. 绵羊SMAD1基因组织表达及其多态性与产羔数关联分析. 中国农业科学. 2019(04): 755-766 .
    6. 田志龙，潘林香，王金文，马琳，储明星. 鲁中肉羊SMAD1和ESR2基因多态性与产羔数关联分析. 中国草食动物科学. 2019(02): 13-16 .
    7. 陈琛，蔺蓓蓓，徐尤美，李鑫鑫，刘祥. 文蛤生物活性多肽/蛋白研究进展. 海洋渔业. 2019(03): 374-384 .

    Other cited types(4)

Catalog

    Get Citation
    PDF
    XML
    Article views (930) PDF downloads (769) Cited by(11)
    Related

    Editorial Office of Acta Hydrobiologica Sinica

    Address：No. 7 Donghu South Road, Wuchang District, Wuhan, Hubei Province, China

    Tel：027-68780701；027-68780800 E-mail：acta@ihb.ac.cn  

    Supported byBeijing Renhe Information Technology Co. Ltd  Technical support：info@rhhz.net

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return