LIN Xiao-Zhi, LUO Yi-Ping, XIE Xiao-Jun. EFFECTS OF DIETARY CARBOHYDRATE LEVEL ON GLYCOLYTIC ENZYMES AND SERUM GLUCOSE CONCENTRATIONS IN THE JUVENILE SOUTHERN CATFISH AFTER FEEDING[J]. ACTA HYDROBIOLOGICA SINICA, 2006, 30(3): 304-310.
Citation: LIN Xiao-Zhi, LUO Yi-Ping, XIE Xiao-Jun. EFFECTS OF DIETARY CARBOHYDRATE LEVEL ON GLYCOLYTIC ENZYMES AND SERUM GLUCOSE CONCENTRATIONS IN THE JUVENILE SOUTHERN CATFISH AFTER FEEDING[J]. ACTA HYDROBIOLOGICA SINICA, 2006, 30(3): 304-310.

EFFECTS OF DIETARY CARBOHYDRATE LEVEL ON GLYCOLYTIC ENZYMES AND SERUM GLUCOSE CONCENTRATIONS IN THE JUVENILE SOUTHERN CATFISH AFTER FEEDING

  • Received Date: November 17, 2005
  • Rev Recd Date: February 17, 2006
  • Published Date: May 24, 2006
  • Three isoenergetic diets containing 0%,15% and 30% gelatinized corn starch were formulated as control, middle level and high level carbohydrate(CHO) diets, respectively. The juvenile southern catfish,Silurus meridionalis Chen(50.5±0.6g)were acclimated with control diet for 15d at 27.5±0.5℃. Activities of glycolytic key enzymes(HK, GKand PFK21) and the serum glucose concentrations in the southern catfish respectively fed with the three diets were examined, at 3,6,12,24 and 48h after feeding. The results showed that HKactivity in the middle and high CHO groups were significantly higher than that in the control group(P<0.05)at 24h,but no significant difference was found at other time; GK activity was related positively with CHO level, and the absolute measurement of its activity was much lower than that of HK;PFK21 activity was not significantly different among the three treatments at each measuring time. The results suggested that the glucose phosphorylation in the fish is catalyzed mainly by HK, but its activity could not be significantly induced by dietary CHO level; GKactivity was induced by dietary CHO level so that glucose phosphorylation was promoted, but efficiency of the promotion was not more than 30%. In addition, activity of PFK21 which would catalyze fructose-6-phosphate into fructose 1,6-bisphosphate did not change at different CHO levels, therefore both metabolic mechanisms in glycolysis could not significantly accelerate with the serum glucose concentrations increasing, which might explain the hyperglycemia in the carnivorous fish with nutrition condition of high dietary CHO level.Serum glucose concentration in either the middle or the high CHO groups primarily increased gradually to its peak at 12h after feeding, and then decreased to the basic level, which could provided experimental basis for designing blood sampling time in this kind of research.
  • [1]
    Hutchins C G, Rawles S D, Gatlin ⅢDM. Effects of dietary carbohydrate kind and level on growth, body composition and glycemic response of juvenile sunshine bass[J].Aquaculture,1998,161:187-199
    [2]
    Shiau S Y. Utilization of carbohydrates in warmwater fish with particular reference to tilapia,Oreochromis niloticus×O. aureus[J].Aquaculture,1997,151:79-96
    [3]
    Lee S M, Lee J H. Effect of dietary glucose, dextrin and starch on growth and body composition of juvenile starry flounderPlatichthys stellatus[J].Fisheries Science,2004,70:53-58
    [4]
    Page G I, Hayworth KM, Wade R R,et al. Non-specific immunity parameters and the formation of advanced glycosylation end-products(AGE) in rainbow trout,Oncorhynchus mykiss (Walbaum),fed high levels of dietary carbohydrates[J].Aquaculture Research, 1999,30(4):287-297
    [5]
    Nordrum S,Bakke-McKellep A M, Krogdahl A,et al. Effects of soybean meal and salinity on intestinal transport of nutrients in Atlantic salmon(Salmo salar L.) and rainbow trout(Oncorhynchus mykiss)[J].Comparative Biochemistry and Physiology B, 2000,125(3):317-32
    [6]
    Deng D F, Refstie S, Hung S S S. Glycemic and glycosuric responses in white sturgeon(Acipenser transmontnus) after oral administration of simple and complex carbohydrates[J].Aquaculture, 2001,199:107-117
    [7]
    Kumar S, Sahu N P, Pal A K,et al. Effect of dietary carbohydrate on haematology,respiratory burst activity and histological changes in juveniles[J].Fish & Shellish Immunology, 2005, 19:331-344
    [8]
    Wilson R P. Utilization of dietary carbohydrate by fish [J].Aqua-culture,1994,124:67-80
    [9]
    MetónI, Mediavilla D, Caseras A,et al, Effect of diet composition and ration size on key enzyme activities of glycolysis-gluconeogenesis,the pentose phosphate pathway and amino acid metabolism in liver of gilthead sea bream(Sparus aurata)[J].British Journal of Nutrition,1999,82:223-232
    [10]
    Caseras A,Metón I, Fernandez F,et al. Glucokinase gene expression is nutritionally regulated in liver of gilthead sea bream(Sparus aurata)[J].Biochimica et Biophysica Acta,2000,1493:135-141
    [11]
    Panserat S, Médale F, Blin C,et al. Hepatic glucokinase is induced by dietary carbohydrates in rainbow trout, gilthead seabream, and common carp[J].American Journal Physiology Regulatory Integrative Comparative Physiology,2000,278:1164-1170
    [12]
    Panserat S, Capill E, Gutierrez J,et al. Glucokinase is highly induced and glucose-6-phosphatase poorly repressed in liver of rainbow trout(Oncorhynchus mykiss) by a single meal with glucose[J].Comparative Biochemistry and Physiology, Part B,2001,128:275-283
    [13]
    Fu S J,Xie XJ. Nutritional homeostasis in carnivorous southern cat-fish(Silurus meridionalis):is there a mechanismfor increased energy expenditure during carbohydrate overfeeding?[J].Comparative Biochemistry and Physiology, Part A,2004,139:359-363
    [14]
    Luo Y P, Xie X J. Effects of dietary starch on the Serum glucose concentrations in Silurus meridionalis Chen.(wait for express)[罗毅平,谢小军. 饲料碳水化合物对南方鲇血糖水平的影响. 2005,(待发表)]
    [15]
    Fu S J, Xie XJ. Effect of dietary carbohydrate levels on growth performance inSilurus meridionalisChen[J].Acta Hydrobiologica Sinica, 2005,29(3) 82-86[付世建,谢小军. 饲料碳水化合物水平对南方鲇生长的影响. 水生生物学报,2005,29(3):82-86]
    [16]
    Fu S J, Xie X J. The kinetic function of blood glucose in southern catfish fed with diets with different dietary carbohydrate levels.(inpress) [付世建,谢小军. 2005b,南方鲇摄食碳水化合物后的血糖动力学方程(待发表)]
    [17]
    Ackerman P A, Forsyth R B, Mazur C F and Iwama G K. Stress hormones and the cellular stress response in salmonids.Fish Physiology and Biochemistry,2000,23:327-336
    [18]
    Xie XJ, Long T C, Zhang Y G,et al. Reproductive investment in theSilurus meridionalis[J].Journal of Fish Biology, 1998, 53:259-271
    [19]
    Kirchner S, Kaushik S, Panserat S. Effect of partial substitution of dietary protein by a single gluconeogenic dispensable amino acid on hepatic glucose metabolism in rainbow trout(Oncorhynchus mykiss)[J].Comparative Biochemistry and Physiology Part A, 2003, 134:337-347
    [20]
    Bradford M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle dye binding[J].Analytical Biochemistry,1976,72:248-254
    [21]
    Seifter S, Dayton S,Novic B,et al. The estimation of glycogen with the anthrone reagent[J].Arch. Biochem. Biophysi,1950,50:191-200
    [22]
    Werve V d, Lange G, Newgard A,et al. New lessons in the regulation of glucose metabolism taught by the glucose-6-phosphatase system[J].European Journal of Biochemistry, 2000,267:1533-1549
    [23]
    Caseras A, Metón I, Vives C,et al. Nutritional regulation of glucose-6-phosphatase gene expression in liver of the gilthead sea bream(Sparus aurata)[J].British Journal of Nutrition,2002,88:607-614
    [24]
    Whiting S J, Wiggs A J. Effect of nutritional factors and cortisoloin tyrosine aminotransferase activitt in liver of brook trout,Salverinus fontinalis mitchill[J].Comparative Biochemistry and Physiology,1977,58B:189-193
    [25]
    Shimeno S, Takeda M, Takayama S,et al. Adaptation of hepatopan-creatic enzymens to dietary carbohydrate in carp[J].Nippon Suisan Gakkaishi, 1981,47:71-77
    [26]
    Moon T W. Glucose intolerance in teleost fish: fact or fiction[J].Comparative Biochemistry and Physiology Part B,2001,129:243-249
    [27]
    Peres H, Goncalves P, Oliva-Teres A. Glucose tolerance in gilthead seabream(Sparus aurata) and European seabass(Dicentrarchus labrax)[J].Aquaculture,1999,179:415-423
    [28]
    Hemre G I, Mommsen T P, Krogdahl A. Carbohydrates in fish nutrition: effects on growth, glucose metabolism and hepatic enzymes[J].Aquacult. Nutrition,2002,8:175-194
    [29]
    Lin J H, Shiau S Y. Hepatic enzyme adaptation to different dietary carbohydrates in juvenile tilapia(Oreochromix niloticu x. O. aureus)[J].Fish Physiology Biochemistry,1995,14:165-170
    [30]
    Lin S C, Liou C-H, Shiau S-Y. Renal threshold for urinary glucose excretion by tilapia in response to orally administered carbohydrates and injected glucose[J].Fish Physiology and Biochemistr,2000,3:127-132

Catalog

    Article views PDF downloads Cited by()
    Related

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return