LU Yi-Shan, NIE Pin. PHYLOGENETIC ANALYSES OF THE MYXOSPOREANS PARASITIC IN FRESHWATER FISH OF CHINA BASED ON 18S RIBOSOMAL DNA[J]. ACTA HYDROBIOLOGICA SINICA, 2004, 28(6): 587-591.
Citation: LU Yi-Shan, NIE Pin. PHYLOGENETIC ANALYSES OF THE MYXOSPOREANS PARASITIC IN FRESHWATER FISH OF CHINA BASED ON 18S RIBOSOMAL DNA[J]. ACTA HYDROBIOLOGICA SINICA, 2004, 28(6): 587-591.

PHYLOGENETIC ANALYSES OF THE MYXOSPOREANS PARASITIC IN FRESHWATER FISH OF CHINA BASED ON 18S RIBOSOMAL DNA

  • Received Date: April 06, 2003
  • Rev Recd Date: May 19, 2004
  • Published Date: November 24, 2004
  • Partial 18S rDNA sequences of five myxosporean species, Myxobolus rotundus,M.rotundatus,Thelohanellus wuhanensis,Henneguya weishanensis and M.cubanicum were amplified utilizing two common primers myxoF(5′-CGCGGTAATTCCAGCTCC AGTAG-3′)and myxoR(5′-ACCAGGTAAGTTTTCCCGTGTTGA-3′)and stored in the GenBank with the access number AY165179-AY165183 By combining the 18S rDNA sequences of other 13 species available in the GenBank a parsimonious tree of 18 species was built with Polypodium hydriforme used as the outgroup.Results show Tetracapsula bryozoides and “PKX"organism lie on the root within the Myxozoa before the divergence of other major groups which form two clades as T.wuhanensis-T. hovorkai-M.rotundatus-M.rotundus-M.bibullatus-M.pellicides-M.pendula-H.salminicola and H.exilis-H. ictaluri-M.spinacurvatura-M.osburni-H.lesteri-H.weishanensis-M.kubanicum. Among the genus myxobolus, 3 species found in China did not form a clade but grouped with the European species,indicating that the geographic divergence of Myxobolus may not be distinct.Neither Myxobolus nor Henneguya was monophyletic and these two genera can not be distinguished using the molecular data.It is further proposed that the caudal protrusion of Henneguya may not be a valid taxonomic character but an appending structure homologous with the “V"projections in Myxobolus.
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    Siddall M E.Whiting M F.Long-branch abstractions[J].Cladistics,1999.15:9-24[2] Anderson C L,Canning E U,Okamura B.A triploblast origin for Myxozoa[J]?Nature,1998,392:346-347[3] Siddall M E,Martin D S,Bridge D,et al.The demise of a phylum of protists:phylogeny of Myxozoa and other parasitic cnidaria[J].J Parasitol,1995,81:961-967[4] Smothers J F,von Dohlen C D,Smith,et al.Molecular evidence that the myxozoan protists are metazoans[J].Science,1994,265:1719-1721[5] Walliker L.The nature of the iodinophilous vacuole of myxosporidian spores and a proposal to synonymize the genus Myxosoma Thelohan,1892 with the genus Myxobolus Butschli,1882[J].J Protozool,1968,15:571-575[6] Lom J.On a new taxonomic character in Myxosporidia,as demonstrated in description of two new species of Myxobolus[J].Folia Parasitol,1969,16:97-103[7] Desser S S,Paterson W B.Ultrastructural and cytochemical observations on sporogenesis of Myxobolus sp.(Myxosporida:Myxobolidae)from the common shiner Notropis cornutus[J].J Protozool,1978,25:314-326[8] Lom J.Hoferellus cyprini(Doflein,1898)Berg,1898(syn:Mitraspora cyprini Fujita,1912),Myxobilatus nostalgicussp.c.and related species:partial revision of two Myxosporean genera[J].Folia Parasitol,1986,33:289-296[9] Lom J.Hoferellus cyprini Doflein,1898 from carp kidney:a well established Myxosporean species or a sequence in the developmental cycle of Sphaerospora renicola Dykova and Lom,1982[J]?Protistol,1985,2:195-206[10] Andree K B,Gresoviac S J,Hedrick R P.Small subunit ribosomal RNA sequences unite alternate actinosporean and myxosporean stages of Myxobolus cerebralis,the causative agent of whirling disease in salmonid fish[J].J Euk Microbiol,1997,44:208-215[11] Anderson C L,Canning E U,Schafer S M,et al.Molecular confirmation of the life cycle of Thelohanellus hovorkai Achmerov,1960(Myxozoa:Myxosporea)[J].Bull Eur Ass Fish Pathol,2000,20:111-114[12] Andree K B,El-Matbouli M,Hoffman R W,et al.Comparison of 18S and ITS-1 sequences of selected geographic isolates of Myxobolus cerebralis[J].Int J Parasitol,1999,29:771-775[13] Andree K B,Szekely C,Molnar K,et al.Relationships among members of the genus Myxobolus(Myxozoa:Bivalvulida)based on small subunit ribosomal DNA sequences[J].J Parasitol,1999,85:68-74[14] Chen Q L,Ma C L.Myxozoa,Myxosporea.Fauna sinica[M].Beijing:Science Press.1998[陈启鎏,马成伦.中国动物志,粘体动物门,粘孢子纲.北京:科学出版社.1998][15] Kent M L,Andree K B,Bartholomew J L,et al.Recent advances in our knowledge of the Myxozoa[J].J Euk Microbiol,2001,48:395-413[16] Sambrook J,Fritsch E F,Maniatis T.Molecular cloning:a laboratory manual,2nd ed[M].New York:Cold Spring Harbor Laboratory Press.1989[17] DNASTAR incorporation.Windows 32 Primerselect,Version 4.01[P].Madison,Wisconsin.1999[18] Thompson J D,Gibson T J,Plewniak F,et al.The ClustalX windows interface:flexible strategies for multiple sequence alignment aided by quality analysis tools[J].Nuc Acids Res,1997,24:4876-4882[19] Felsenstein J.PHYLIP-Phylogeny Inference Package,Version 3.6(alpha3)[P],Seattle,Washington:Department of Genome Sciences,University of Washington.2002[20] Anderson C L,Canning E U,Okamura B.18S rDNA sequences indicate that "PKX"organism parasitizes Bryozoa[J].Bull Eur Ass Fish Pathol.1999,19:94-97[21] Anderson C L,Canning E U,Okamura B.Molecular data implicate bryozoans as host for "PKX"(Phylum Myxozoa)and identify a clade of bryozoan parasites within the Myxozoa[J].Parasitology.1999,119:556-561[22] Canning E U,Okamura B,Curry A.Development of a myxozoan parasite Tetracapsula bryozoides gen.c.et sp.n.in Cristatella mucedo(Bryozoa:Phylactolaemata)[J].Folia Parasitol,1996,43:249-261

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