YE Yu-Zhen, ZHOU Jian-Feng, WANG Zhong-Wei, ZHANG Jing, WEI Wen-Hui. COMPARATIVE STUDIES ON THE DNA CONTENT FROM THREE STRAINS OF CRUCIAN CARP (CARASSIUS AURATUS)[J]. ACTA HYDROBIOLOGICA SINICA, 2004, 28(1): 13-16.
Citation: YE Yu-Zhen, ZHOU Jian-Feng, WANG Zhong-Wei, ZHANG Jing, WEI Wen-Hui. COMPARATIVE STUDIES ON THE DNA CONTENT FROM THREE STRAINS OF CRUCIAN CARP (CARASSIUS AURATUS)[J]. ACTA HYDROBIOLOGICA SINICA, 2004, 28(1): 13-16.

COMPARATIVE STUDIES ON THE DNA CONTENT FROM THREE STRAINS OF CRUCIAN CARP (CARASSIUS AURATUS)

  • Received Date: November 11, 2002
  • Rev Recd Date: December 27, 2002
  • Published Date: January 24, 2004
  • In China, there are many strains of cruc ian carp in different area. According to genetic theory, the chromosomal numbers and D NA contents of different species can reflect the relationships among species bec ause of invariableness of themselves. Generally, cellular DNA content was determined by Feulgen staining and micro-fl uorescence dense miter. However, its manipulation is lasting and complicated by this method. In present paper, using flow cytometry (FCM) the DNA contents of er ythrocytes from red crucian carp (Carassius auratus var. red),Pengze crucian carp (Carassius auratus var. Pengze)and gibel carp (Carassius auratus gib elio)were measured and comparisons between them were carried. Four live fishes from every strain were collected from Guanqiao experimental Station of Institut e of Hydrobiology, the Chinese Academy of Sciences. From every experimental fish 0.5mL of blood were collected, and erythrocytes were isolated by means of prec ipitation. Cock red cell was served as control. After raising and PI staining, D NA contents of erythrocytes were measured by flow cytometry (FCM, Beckman Coulte r).Measuring dates were treated and assayed by microcomputer. The DNA contents of erythrocytes from red crucian carp (Carassius auratus va r. red),pengze crucian carp (C. auratus var pengze) and allogynogenetic cru cian carp (C. arasstus gibelio)were measured and comparisons between them we re carried. Results indicated that DNA content of erythrocyte was 3.0 picograms (pg) in red crucian carp, 4.7 picograms(pg) in pengze crucian carp, and 4.8 pi cograms (pg) in allogynogenetic crucian carp. Obviously, DNA content of triploid pengze crucian carp was 1.57 times of diploid red crucian carp, and content of allogynogenetic crucian carp was 1.6 times of diploid red crucian carp. Ploidy investigation of red crucian carp, pengze crucian carp and allogynogenetic cruc ian carp was undertaken by chromosomal number counts of mitosis metaphase of kid ney cell. The result showed that chromosomal number of red crucian carp was dipl oidy (2n=100), chromosomal number of Pengze crucian carp was triploidy(3n=162) a nd chromosomal number of allogynogenetic crucian carp was from 156 to 162(3n=156—162). The research revealed that there was a significantly positive correlatio n between DNA content and ploidy. Use of flow cytometry technique as ploidy dis crimination of fish have some advantages (convenience, rapidity, credibility) an d made up some shortages of chromosomal analysis. It provided new methods and th eoretical basis for checking up of quality of fish. At the same time, it can also be used as ploidy discrimination of rare freshwater and marine and introduced polyploidy fish.
  • [1]
    Wu Q J, Ye Y Z, Chen R D. Karyotype evidences of genome manipulation in the carp (Cyprinus carpio L.)[J].Acta Hydrobiologica Sinica,1986,10(3):265-270. [吴清江,叶玉珍,陈荣德. 鲤鱼染色体组调控的核型证明. 水生生物学报,1986,10(3):265-270][2] Ye Y Z, Wu Q J, Chen R D. Studies in cytology of crosses between grass carp and carp-asynchronization between nucleus and cytoplasm in distant hybridization of fishes [J]. Acta Hydrobiologica Sinica,1989,13(3):234-239. [叶玉珍,吴清江.草鱼和鲤鱼杂交的细胞学研究--鱼类远缘杂交核质不同步现象. 水生生物学报,1989,13(3):234-239][3] Wu Y P, Ye Y Z, Wu Q J. Studies on the induction of allotriploid using heat shock in zebrafish, Brachydanio frankei and Brachydanio rerio [J]. Oceanologia Et Limnologia Sinica.2000,31(5):465-470.[吴玉萍,叶玉珍,吴清江. 热休克诱导斑马鱼异源三倍体的研究. 海洋与湖沼,2000,31(5):465-470][4] Preniva G Mazzini G, Cora S. Methodological and instrumentation aspect of cytofleuorometry [J].Histochemical.1974,6:259-278[5] Li Y C, Li K, Zhou D. Cellular DNA content of fourteen species of fresh-water fishes[J].Acta Genetica Sinica, 1983,10(5):384-389.[李渝成,李康,周暾.十四种淡水鱼的DNA含量. 遗传学报,1983,10(5):384-389][6] Ye Y Z, Wu Q J. Relative and content measurement and ploidy analysis of artificial multiple triploid carp and its three parental forms [J].Acta Hydrobiologica Sinica. 1998,22(2):119-122. [叶玉珍,吴清江. 人工复合三倍体鲤与亲本相对DNA含量及倍性分析. 水生生物学报1998,22(2):119-122][7] Yu X M, Chen M R, Yang X Q, et al. Observation on erythrocytes and contents in artificially-induced allotetraploid and interploid triploid Japanese phytophagous crucian carp[J].Acta Hydrobiologica Sinica.1998,22(3):291-294.[俞小牧,陈敏容,杨兴棋,等. 人工诱导异源四倍体和倍间三倍体白鲫的红细胞观察及其相对DNA含量测定. 水生生物学报,1998,22(3):291-294][8] Frederick J A, Robert Q M, Jerome V S. Induced triploids and tetraploid in bighead carp, Hypophthalmichthys nobilis,Verified by multi-embryo cytofuorometric analysis[J]. Aquculture, 1990,87,121-131[9] Xiao Y M, Luo C, Liu Y, et al. Ploid analysis of the gynogenetic grass carps[J]. Life Science Research,2001,5(4):290-293.[肖亚梅,罗琛,刘筠,等.人工雌核发育草鱼染色体倍性的鉴定. 生命科学研究,2001,5(4):290-293][10] Fan Z T, Yin H B, Song S X. DNA content from thirteen species of cultured freshwater fishes in China[J].Jour. of Fisheries of China, 1995,19(4):322-327.[范兆廷. 十三种淡水养殖鱼类的DNA含量. 水产学报,1995, 19(4):322-327]

    Wu Q J, Ye Y Z, Chen R D. Karyotype evidences of genome manipulation in the carp (Cyprinus carpio L.)[J].Acta Hydrobiologica Sinica,1986,10(3):265-270. [吴清江,叶玉珍,陈荣德. 鲤鱼染色体组调控的核型证明. 水生生物学报,1986,10(3):265-270][2] Ye Y Z, Wu Q J, Chen R D. Studies in cytology of crosses between grass carp and carp-asynchronization between nucleus and cytoplasm in distant hybridization of fishes [J]. Acta Hydrobiologica Sinica,1989,13(3):234-239. [叶玉珍,吴清江.草鱼和鲤鱼杂交的细胞学研究--鱼类远缘杂交核质不同步现象. 水生生物学报,1989,13(3):234-239][3] Wu Y P, Ye Y Z, Wu Q J. Studies on the induction of allotriploid using heat shock in zebrafish, Brachydanio frankei and Brachydanio rerio [J]. Oceanologia Et Limnologia Sinica.2000,31(5):465-470.[吴玉萍,叶玉珍,吴清江. 热休克诱导斑马鱼异源三倍体的研究. 海洋与湖沼,2000,31(5):465-470][4] Preniva G Mazzini G, Cora S. Methodological and instrumentation aspect of cytofleuorometry [J].Histochemical.1974,6:259-278[5] Li Y C, Li K, Zhou D. Cellular DNA content of fourteen species of fresh-water fishes[J].Acta Genetica Sinica, 1983,10(5):384-389.[李渝成,李康,周暾.十四种淡水鱼的DNA含量. 遗传学报,1983,10(5):384-389][6] Ye Y Z, Wu Q J. Relative and content measurement and ploidy analysis of artificial multiple triploid carp and its three parental forms [J].Acta Hydrobiologica Sinica. 1998,22(2):119-122. [叶玉珍,吴清江. 人工复合三倍体鲤与亲本相对DNA含量及倍性分析. 水生生物学报1998,22(2):119-122][7] Yu X M, Chen M R, Yang X Q, et al. Observation on erythrocytes and contents in artificially-induced allotetraploid and interploid triploid Japanese phytophagous crucian carp[J].Acta Hydrobiologica Sinica.1998,22(3):291-294.[俞小牧,陈敏容,杨兴棋,等. 人工诱导异源四倍体和倍间三倍体白鲫的红细胞观察及其相对DNA含量测定. 水生生物学报,1998,22(3):291-294][8] Frederick J A, Robert Q M, Jerome V S. Induced triploids and tetraploid in bighead carp, Hypophthalmichthys nobilis,Verified by multi-embryo cytofuorometric analysis[J]. Aquculture, 1990,87,121-131[9] Xiao Y M, Luo C, Liu Y, et al. Ploid analysis of the gynogenetic grass carps[J]. Life Science Research,2001,5(4):290-293.[肖亚梅,罗琛,刘筠,等.人工雌核发育草鱼染色体倍性的鉴定. 生命科学研究,2001,5(4):290-293][10] Fan Z T, Yin H B, Song S X. DNA content from thirteen species of cultured freshwater fishes in China[J].Jour. of Fisheries of China, 1995,19(4):322-327.[范兆廷. 十三种淡水养殖鱼类的DNA含量. 水产学报,1995, 19(4):322-327]

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