ZHANG Jie, XU Ying, LI Wen, WU Wen-zhong. STUDIES ON RETINIODS AND α-TOCOPHEROL IN LIVER OF SILVER CARP(Hypophthalmichthys molitrix)FROM YAN-JIA LAKE-HIGH ORANOCHLORINE POLLUTED LAKE[J]. ACTA HYDROBIOLOGICA SINICA, 2001, 25(2): 105-110.
Citation: ZHANG Jie, XU Ying, LI Wen, WU Wen-zhong. STUDIES ON RETINIODS AND α-TOCOPHEROL IN LIVER OF SILVER CARP(Hypophthalmichthys molitrix)FROM YAN-JIA LAKE-HIGH ORANOCHLORINE POLLUTED LAKE[J]. ACTA HYDROBIOLOGICA SINICA, 2001, 25(2): 105-110.

STUDIES ON RETINIODS AND α-TOCOPHEROL IN LIVER OF SILVER CARP(Hypophthalmichthys molitrix)FROM YAN-JIA LAKE-HIGH ORANOCHLORINE POLLUTED LAKE

  • Received Date: December 27, 1999
  • Rev Recd Date: February 19, 2000
  • Published Date: March 24, 2001
  • Retinol, retinyl palmitate and a-tocopherol were measured by reverse-phase HPLC in liver of silver carp(Hypophthalmichthys molitrix)from Yanjia Lake, high organochlorine polluted lake, in summer and winter, 1999. The results indicated that the biotransformation/metabolism of two nutriments were affected by ambient' temperature. In summer while the temperature was higher, vitamins were mainly in their active forms, hence retinol and tocopherol levels in livers of silver carp were higher. But in winter while the temperature was lower, they were mostly in deposited forms, so retinyl palmitat was at higher level than that in summer. Furthermore, increasing trends of retinoids was significantly found in those Samples from pond 1 to pond 5, showing a good dose-response relationship between water contamination and their toxic effects. It is considered that retiniods in fish liver might be used as an effective biomarker in assessing toxic effects of organochlorine pollution in waters.
  • [1]
    Vince P P,Scott B B.HPLC determination of Tocopherol,retinol,dehydroretinol and retinyl palmitate in tissues of lake char(Salvelinus namaycush)exposed to coplanar 3,3',4,4',5-pentachlorobiphenyl[J].Environ.Toxicol.Chem.1994,13:473—476[2] Harry T B.,Esther E F,Martin L E,et al.Alteration in plasma and hepatic retinoid levels in flounder(platichthys flesus)after chronic exposure to contaminated harbour sludge in a mesocosm study[J].Aquat.Toxico,1981,42,271—285[3] Stohs S J,Shara M A,Alsharif N z,et al.2,3,7,8-tetrachlorodibenzo-p-dioxin-induced oxidative stress in female rats.[J].Toxicol.Appl.Pharmacol.,1990,106:126—135[4] Helen H.The role of retinoids in dioxin toxicity.Dioxin'97,Indianapolis,Indiana,USA.1997,34:402—405[5] Hakansson H,Manzoor E Ahlborg U G.Interaction between vitamin A and single oral doses of 2,3,7,8-tetrachlorodibenzo-p-dioxin(TCDD)on the TCDD-induced toxity and on the vitamin A status in the rat [J] Nutr.Sci.Vitaminol.1991,37:239—255[6] Arnaud J.,Fortis I.S.Blachier,et al.Simultaneous determination of retinol,α-tocopherol and β-carotene inserum by isocratic high-performance liquid chromatography.[J].J.Chromatogr.1991,572:103—116[7] Spear P A,Bilodeau A Y,Branchard A.Retinoids:From metabolism to environmental monitoring[J].Chemosphere,1992,25:1733—1738[8] Xu Y,Zhang Y Y,Oxynos K.et al.Hexachlorocyclohexane(HCH)residues in Ya-Er lake area,China[J] International Journal of Environmental Analytical Chemistry,1994,57:53—61[9] Wu W Z,Schramm K.-W,Xu Y,et al.PCDD/Fs,PCBs,HCHs and HCB in Sediment and soils of Ya-Er Lake Area in China:Results on Residual levels and correlation to the organic carbon and particle size [J],Chemoshpere,1997,34:191—202[10] Chen G S,Schramm K.-W,Xu Y,et al.Polycyclic Aromatic Hydrocarbon in Ya-Er Lake(Hubei,China):Source and Distribution [J].Fresenius Journal of Analytical Chemistry,1997,359:280—284[11] Chen G S,Xu Y,Xu L H,et al.Influence of dioxin and metal-contaminated sediment on phase I and II biotransformation enzymes in silver crucian carp [J].Ecotoxicology and Environmental safety,1998,40:234—238[12] 徐 盈,吴文忠,张银华,等.湖北鸭儿湖地区六氯环己烷的残留动态与长期归宿研究,水生生物学报,1999,23(4):337—345

    Vince P P,Scott B B.HPLC determination of Tocopherol,retinol,dehydroretinol and retinyl palmitate in tissues of lake char(Salvelinus namaycush)exposed to coplanar 3,3',4,4',5-pentachlorobiphenyl[J].Environ.Toxicol.Chem.1994,13:473—476[2] Harry T B.,Esther E F,Martin L E,et al.Alteration in plasma and hepatic retinoid levels in flounder(platichthys flesus)after chronic exposure to contaminated harbour sludge in a mesocosm study[J].Aquat.Toxico,1981,42,271—285[3] Stohs S J,Shara M A,Alsharif N z,et al.2,3,7,8-tetrachlorodibenzo-p-dioxin-induced oxidative stress in female rats.[J].Toxicol.Appl.Pharmacol.,1990,106:126—135[4] Helen H.The role of retinoids in dioxin toxicity.Dioxin'97,Indianapolis,Indiana,USA.1997,34:402—405[5] Hakansson H,Manzoor E Ahlborg U G.Interaction between vitamin A and single oral doses of 2,3,7,8-tetrachlorodibenzo-p-dioxin(TCDD)on the TCDD-induced toxity and on the vitamin A status in the rat [J] Nutr.Sci.Vitaminol.1991,37:239—255[6] Arnaud J.,Fortis I.S.Blachier,et al.Simultaneous determination of retinol,α-tocopherol and β-carotene inserum by isocratic high-performance liquid chromatography.[J].J.Chromatogr.1991,572:103—116[7] Spear P A,Bilodeau A Y,Branchard A.Retinoids:From metabolism to environmental monitoring[J].Chemosphere,1992,25:1733—1738[8] Xu Y,Zhang Y Y,Oxynos K.et al.Hexachlorocyclohexane(HCH)residues in Ya-Er lake area,China[J] International Journal of Environmental Analytical Chemistry,1994,57:53—61[9] Wu W Z,Schramm K.-W,Xu Y,et al.PCDD/Fs,PCBs,HCHs and HCB in Sediment and soils of Ya-Er Lake Area in China:Results on Residual levels and correlation to the organic carbon and particle size [J],Chemoshpere,1997,34:191—202[10] Chen G S,Schramm K.-W,Xu Y,et al.Polycyclic Aromatic Hydrocarbon in Ya-Er Lake(Hubei,China):Source and Distribution [J].Fresenius Journal of Analytical Chemistry,1997,359:280—284[11] Chen G S,Xu Y,Xu L H,et al.Influence of dioxin and metal-contaminated sediment on phase I and II biotransformation enzymes in silver crucian carp [J].Ecotoxicology and Environmental safety,1998,40:234—238[12] 徐 盈,吴文忠,张银华,等.湖北鸭儿湖地区六氯环己烷的残留动态与长期归宿研究,水生生物学报,1999,23(4):337—345
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