ZHUO Xiao-Lei, ZOU Ji-Xing, CUI Ke, ZHONG Shan, HE Xue-Jun. THE INFLUENCE OF EXOGENOUS METHYLTESTOSTERONE ON THE GONAD DEVELOPMENTAL PROCESS OF FEMALE AND INTERSEXUAL MONOPTERUS ALBUS[J]. ACTA HYDROBIOLOGICA SINICA, 2008, 32(6): 861-867.
Citation: ZHUO Xiao-Lei, ZOU Ji-Xing, CUI Ke, ZHONG Shan, HE Xue-Jun. THE INFLUENCE OF EXOGENOUS METHYLTESTOSTERONE ON THE GONAD DEVELOPMENTAL PROCESS OF FEMALE AND INTERSEXUAL MONOPTERUS ALBUS[J]. ACTA HYDROBIOLOGICA SINICA, 2008, 32(6): 861-867.

THE INFLUENCE OF EXOGENOUS METHYLTESTOSTERONE ON THE GONAD DEVELOPMENTAL PROCESS OF FEMALE AND INTERSEXUAL MONOPTERUS ALBUS

  • Received Date: June 24, 2007
  • Rev Recd Date: April 28, 2008
  • Published Date: November 24, 2008
  • This paper studied the influence of the exogenous androgen on the gonad developmental process of female and intersexual Monopterus albus after being treated with large dosage of methyltestone(MT),by the method of gonad biopsy.We dissolved the exogenous androgen with salad oil,and then injected them into the abdominal cavity of female and intersexual Monopterus albus.The influence of the exogenous androgen on the gonad developmental process of the female and intersexual Monopterus albus was studied by light-microscope.The conclusion might lay the foundation for further revealing mystery of the sex-reversal,and we found some interesting structures in histology,although we could not get the sex-reversal male of Monopterus albus by inducing artificially.The results were as follows:① MT could promote the degradation of the oocyte of the female Monopterus albus,but it could not make the ovarian tissue of the female Monopterus albus degenerated completely.② MT could promote the enlargement and extension of the epigynum of the female and intersexual Monopterus albus,but it could not make the interstitial cells of the female and intersexual Monopterus albus proliferated.③ MT could promote the appearance of the "hollow lobules" on the epigynum of the female and intersexual Monopterus albus.These "hollow lobules" could be divided into two types,the Hollow lobule type Ⅰ("HL-Ⅰ") and the Hollow lobule type Ⅱ("HL-Ⅱ").And the Hollow lobule type Ⅱ may be the seminiferous lobules,which were produced under the abnormal development.④ MT could promote the development of male reproductive cells in the early period of the intersexual Monopterus albus.And it could even promote the excreting of male reproductive cells in seminiferous lobules in the late period of the intersexual Monopterus albus,with the emergence of the other type of the "Hollow lobule".⑤ The influence of MT on the gonad developmental process of the intersexual Monopterus albus was better than that of the female Monopterus albus.
  • [1]
    L iu C K. Rud im entary h erm aph rod itism in the symb rancho id ee,lM onopterus javanen sis [J]. S in en sia, 1944, 15: 1-8
    [2]
    Davis K B, Ludw ig G M. H orm on al effects on sex d ifferent iationand grow th in sun sh ine bass Moron e chrysops-m orone saxa tilis [J]. Aquacul ture, 2004, 231: 587-596
    [3]
    Andersen L, H o lbech H, Gessbo A, et a l. E f fects of exposure to17 A-eth iny lestrad iol during early developm ent on sexual differentiat ion and indu ct ion of vitellogen in in zebraf ish (Dan io reio ) [J]. C omp B iochem PhysiolC, 2003, 134: 365-374
    [4]
    L iu J K, Gu G Y. The change ofM onopteru s a lbus gon ad on thesex reversion [J]. Jou rnal of H yd robiology of China, 1951,2( 1) 2 ): 85-109 [刘建康, 顾国彦. 黄鳝性逆转时生殖腺组织的改变. 中国水生生物学汇报, 1951, 2 ( 1) 2 ) : 85-109]
    [5]
    L iem K F. Sex reversal as a natu ral process in the sym branch-form f ishM on op terus albu s[J]. Copeia, 1963, 2: 303-312
    [6]
    Tao Y X, Lin H R, G len V D K, et a l. H orm on al induction ofp recocious sex reversal in th e ricefield ee,l M onop teru s albue [J]. Aquacu ltu re, 1993, 118: 131-140
    [7]
    Chen L L, X iao Y M, Liu Y. A report abou t th e new developmen t of gonad inm on op teru s albue [J]. A ctaH yd robiolog ica S inica,2006, 30 ( 5) : 621-624[陈丽莉, 肖亚梅, 刘筠. 黄鳝一种新的生殖腺发育状况报道. 水生生物学报, 2006, 30 ( 5) :621-624]
    [8]
    Xiao Y M, L iuY. S tudy on the h istology in sex changing from intersexto m ale ofM onopteru s albu s( Zu iew ) [J]. Journa l of F isheriesof C hina, 1995, 19 ( 4 ): 297-304 [肖亚梅, 刘筠. 黄鳝由间性发育转变为雄性发育的细胞生物学研究. 水产学报,1995, 19( 4 ): 297-304]
    [9]
    M eiW X, Cao Y L, L iR L, et al. A prel im in ary ob servation onsex reversal of th em ud eelM onopteru s a lbu s[J]. Journa l of ZhejiangColleg e of F ish eries, 1993, 12 ( 1) : 53-58 [梅文骧, 操应龙, 李蕊蕾, 等. 黄鳝M ono-p terus albu s性转变的探讨. 浙江水产学院学报, 1993, 12( 1) : 53-58]
    [10]
    YangW Y, Gu Z Q, W ang CH, et a l. Observat ion of gonad h istologyin the course of the sex reverses for th e eels(H om opterou salbum s) [J]. P rogress in Veterinary M ed icin e, 2004, 25 ( 6 ) :113-115 [杨文云, 顾忠旗, 王春华, 等. 黄鳝性逆转过程中性腺形态学初步观察. 动物医学进展, 2004, 25 ( 6 ) : 113)115]
    [11]
    Zh ou Q B, W u H D, W uH X, e t a l. The effect of spaw n andnot spawn on sex reversal in ricef ield eel [J]. Journa l of E conomicA nima l, 2004, 8 ( 2) : 89-91 [周秋白, 吴华东, 吴红翔, 等. 产卵与黄鳝性转化关联研究. 经济动物学报, 2004,8( 2 ) : 89-91]
    [12]
    L iu X Y, Cu iT C, Wang L C, et a l. H istological and u ltrastructuralchanges in the gonad during sex reversal inM on op terus a lbus [J]. A ctaH yd robiolog ica S in ica, 1990, 14 ( 2) : 166-171 [刘修业, 崔同昌, 王良臣, 等. 黄鳝性逆转时生殖腺的组织学与超微结构的变化. 水生生物学报, 1990, 14 ( 2) : 166-171]
    [13]
    Tao Y X, L inH R. S tud ies on the natu ral sex reversal of paddyfieldee,l M onop teru s a lbu s [J]. A cta H ydrobiolog ica S in ica,1991, 15( 3 ) : 274-278 [陶亚雄, 林浩然. 黄鳝自然性反转的研究. 水生生物学报, 1991, 15( 3) : 274-278]
    [14]
    Lv D Y, Song P, C henY G, e t al. Expression of gene va sa duringgonadal natural sex reversal of rice field eelm onopterus a lbus [J]. Acta Zoolog ica S inica, 2005, 51 ( 3 ) : 467-475 [吕道远, 宋平, 陈云贵, 等. 黄鳝性腺自然逆转过程中va sa 基因的表达分析. 动物学报, 2005, 51( 3 ): 467-475]
    [15]
    Ch an S T H, W a-i Sum O, Tang F, e t al. B iopsy stud ies on thenatu ral sex reversal inM onopteru s a lbu s ( P isces: T eleostei) [J].Journa l of Z oology (L ond on ), 1972, 167: 415-421
    [16]
    Tang F, Lofts B, Chan S T H. A s-3 f-l hyd roxysteroid dehyd rogenaseact iv it ies in the ovaries of ricefield ee,l M onopteru s a lbus.( Zuiew ) [J]. Exp erientia, 1974, 30: 316-317
    [17]
    Zhang X X, Dong Y K. A p relim inary study on sex induction byexogenou sm ale steroid horm one du ring larva stage ofmonop terusa lbus ( p isces, teleostei) [J]. R eservoir F isheries, 1994, 6:18-20 [张小雪, 董元凯. 外源雄性激素对幼鳝性别诱导的初探. 水利渔业, 1994, 6: 18-20]

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