Volume 31 Issue 4
Aug.  2014
Turn off MathJax
Article Contents
Abstract
References
WEN Chong-Qing, LAI Xin-Tian, HE Hong, XUE Ming, ZHOU Shi-Ning. SCREENING AND IDENTIFICATION OF TWO PROBIOTIC BACILLUS STRA[J]. ACTA HYDROBIOLOGICA SINICA, 2007, 31(4): 453-459.
Citation: WEN Chong-Qing, LAI Xin-Tian, HE Hong, XUE Ming, ZHOU Shi-Ning. SCREENING AND IDENTIFICATION OF TWO PROBIOTIC BACILLUS STRA[J]. ACTA HYDROBIOLOGICA SINICA, 2007, 31(4): 453-459.
shu

SCREENING AND IDENTIFICATION OF TWO PROBIOTIC BACILLUS STRA

  • Fisheries College, Guangdong Ocean University, Zhanjiang 524088;
    2. School of Life Sciences, Sun Yat-sen University, Guangzhou 510275;
    3. Agricultural College, Guangdong Ocean University, Zhanjiang 524088

  • Received Date: January 05, 2006
  • Rev Recd Date: January 24, 2007
  • Published Date: July 24, 2007
    Graphical Abstract
    • Abstract
  • 22 strains of Bacillus with different characters were isolated from soil and shrimp ponds. Approximately 5 ×108 logarithmic phase cells of every strain were administered into 500 mL seawater culturing Liptopenaeus vannamei nauplius m larvae respectively. Based on the effect of Bacillus on the survival rate of L. vannamei larvae metamorphosis from nauplius m to zoea Ⅱ, 10 strains which had very significant (p<0.01 ) effects on larval metamorphosis were screened initially. Then from the 10 strains screened, two strains, zou4 and zou8 which had more significant (p<0.05) effects on larval metamorphosis than other strains were selected as potential probiotic Bacillus for L. vannamei larvicuhure. Combining phenotypic and physiological characteristics, zou4 and zou8 were identified initially as B. firmus and B. cereus respectively. To investigate the phylogenetic posi-tion of strains zou4 and zou8, about 1.5kb 16S rRNA genes were sequenced and compared with that of related strains, which re-vealed that strains zou4 and zou8 exhibited the highest levels of similarity to B. firmus and B. cereus respectively, similarity value were all above 99 %. Phylogenetic tree was constructed based on genetic distance analysis, which also showed that strains zou4 and zou8 clustered as B. firmus and B. cereus respectively. Based on the results obtained, strains zou4 and zou8 were identified as B. firmus and B. cereus respectively.
    • FullText(HTML)
    • References (20)
  • [1]
    Gatesoupe F G. The use of probiotics in aquaculture[J]. Aquaculture, 1999, 180: 147-165
    [2]
    Verschuere L, Romaut G, Sorgeloos P, et al. Probiotic bacteria as biological control agents in aquaculture[J]. Microbiology and Molecular Biology Review, 2000, 64 (4): 655-671
    [3]
    Gomez-Gil B, Roque A, Turnbull J F. The use and selection of probiotic bacteria for use in the culture of larval aquatic organisms[J].Aquaculture, 2000, 191: 259-270
    [4]
    Skjermo J, Vadstein O. Techniques for microbial control in the intensive rearing of marine larvae[J]. Aquaculture, 1999, 177: 333-343
    [5]
    Moriarty D J W. Control of luminous Vibrio species in penaeid aquaculture ponds[J]. Aquaculture, 1998, 164: 351-358
    [6]
    Rengpipat S, Phianphak W, Piyatiratitivorakul S, et al. Effects of aprobiotic bacterium on black tiger shrimp Penaeus monodon survivaland growth[J]. Aquaculture, 1998, 167: 301-313
    [7]
    Devaraja T N, Yusoff F M, Shariff M. Changes in bacterial populations and shrimp production in ponds treated with commercial microbial products[J]. Aquaculture, 2002, 206: 245-256
    [8]
    Vaseeharan B, Ramasamy P. Control of pathogenic Vibrio spp. by Bacillus subtilis BT23, a possible probiotic treatment for black tigershrimp Penaeus monodon[J]. Letters in Applied Microbiology,2003, 36 (2): 83-87
    [9]
    Liu F, Han W Y. Reuse strategy of wastewater in prawn nursery by microbial remediation[J]. Aquaculture, 2004, 230: 281-296
    [10]
    Gullian M, Thompson F, Rodriguez J. Selection of probiotic bacteria and study of their immunostimulatory effect in Penaeus vannamei[J]. Aquaculture, 2004, 233: 1-14
    [11]
    Dong X Z, Chai M Y, et al. Manual of systematic determination of familiar bacteria[M]. Beijing: Science Press. 2001, 62-65,353-391[东秀珠,蔡妙英,等.常见细菌系统鉴定手册.北京:科学出版社. 2001, 62-65, 353-391]
    [12]
    Oggioni M R, Ciabattini A, Cuppone A M, et al. Bacillus spores for vaccine delivery[J]. Vaccine, 2003, 21: 96-101
    [13]
    Sanders M E, Morelli L, Tompkins T A. Sporeformers as human probiotics: Bacillus, Sporolactobacillus, and Brevibacillus[J]. Comprehensive Reviews in Food Science and Food Safety, 2003, 2: 101-110
    [14]
    Spanggaard B, Huber I, Nielsen J, et al. The probiotic potential against vibriosis of the indigenous microflora of rainbow trout[J].Environmental Microbiology, 2001, 3 (12): 755-765
    [15]
    Hjelm M, Berg, Riaza A, et al. Selection and identification of autochthonous potential probiotic bacteria from turbot larvae (Scophthalmus maximus) rearing units[J]. Systematic and Applied Microbiology, 2004, 27: 360-371
    [16]
    Gram L, L vold T, Nielsen J, et al. In vitro antagonism of the probiont Pseudomonas fluorescens strain AH2 against Aeromonas salmonicida does not confer protection of salmon against furunculosis[J].Aquaculture, 2001, 199: 1-11
    [17]
    Green D H, Wakeley P R, Page A, et al. Characterization of two Bacillus probiotics[J]. Applied and Environmental Microbiology,1999, 65 (9): 4288-4291
    [18]
    Hoa N T, Baccigalupi L, Uxham, H, et al. Characterization of Bacillus species used for oral bacteriotherapy and bacterioprophylaxisof gastrointestinal disorders[J]. Applied and Environmental Microbiology, 2000, 66 (12): 5241-5247
    [19]
    Taylor J M W, Sutherland A D, Aidoo K E, et al. Heat-stable toxin production by strains of Bacillus cereus, Bacillus firmus, Bacillus megaterium, Bacillus simplex and Bacillus licheniformis[J]. FEMSMicrobiology Letters, 2005, 242: 313-317
    [20]
    Health and nutritional properties of powder milk and live lactic acid bacteria, 124 October 2001, Córdoba, Argentina[C]. 2001, 5-7
    • Related Articles

  • Cited by

    Periodical cited type(18)

    1. 瞿飞虎，姜晓娜，葛彦龙，翟钰姗，张玲，王继瑶，寇寒，李池陶，胡雪松，石潇丹，贾智英. 黄河鲤对饲料蛋白质需求量研究. 南方水产科学. 2024(04): 124-132 .
    2. 易婉婷，华雪铭，颜克涛，宓水潮，蓝汉冰，杭莹，丛湘明. 酵母水解物与小肽对大口黑鲈幼鱼生长性能、肝肠健康与抗感染能力的影响. 上海海洋大学学报. 2022(05): 1097-1107 .
    3. 曾本和，刘海平，王建，王万良，周建设，王金林，朱成科，张忭忭. 饲料蛋白质水平对拉萨裸裂尻鱼幼鱼肌肉氨基酸及蛋白质代谢的影响. 中国水产科学. 2019(06): 1153-1163 .
    4. 徐兆利，曾本和，杨瑞斌，牟振波，李宝海，王万良，张忭忭，刘海平. 饲料蛋白质水平对拉萨裸裂尻鱼幼鱼免疫和抗氧化能力的影响. 动物营养学报. 2019(12): 5645-5654 .
    5. 赵书燕，林黑着，黄忠，周传朋，王珺，王芸，戚常乐. 不同蛋白水平对2种规格石斑鱼生长性能、血清生化及肌肉品质的影响. 南方水产科学. 2017(04): 87-96 .
    6. 孙金辉，范泽，张美静，程镇燕，白东清，乔秀亭. 饲料蛋白水平对鲤幼鱼肝功能和抗氧化能力的影响. 南方水产科学. 2017(03): 113-119 .
    7. 孔纯，华雪铭，杨璐，刘韬，杨景丰，王坛，王刚，吴钊，施永海，税春，苏美英. 暗纹东方鲀饲料中豆粕替代鱼粉的营养生理效应及其与大豆抗原蛋白的相关性. 水产学报. 2017(05): 734-745 .
    8. 李宗升，吴立新，李思萌，彭博文，赵博文. 鸡肉粉替代鱼粉对大菱鲆生长和体组成的影响. 水产科学. 2016(05): 486-491 .
    9. 王荻，刘红柏. 施氏鲟抗热应激中草药免疫制剂的筛选. 大连海洋大学学报. 2016(06): 635-639 .
    10. 吴金平，刘伟，蒋明，文华，吴凡，田娟，杨长庚. 大规格吉富罗非鱼幼鱼对饲料中胆碱的需要量. 动物营养学报. 2016(01): 256-264 .
    11. 王坛，华雪铭，朱伟星，吴钊，孔纯，何亚丁，苏美英. 饲料溶菌酶添加水平对氨氮应激下吉富罗非鱼血清生化指标、抗菌性能和肝脏抗氧化能力的影响. 水产学报. 2016(05): 740-750 .
    12. 王荻，廉超，刘红柏. 中草药添加剂对施氏鲟生长及血液生化指标的影响. 水产学杂志. 2016(03): 16-20 .
    13. 王震，徐玮，麦康森，路凯，刘迎隆，艾庆辉. 饲料缬氨酸水平对军曹鱼鱼体脂肪含量、血浆生化指标和肝脏脂肪代谢基因表达的影响. 水生生物学报. 2016(04): 744-751 . 本站查看
    14. 吴金平，文华，蒋明，刘伟，吴凡，田娟，黄凤，任春. 生物素对吉富罗非鱼幼鱼生长、体成分及血清生化指标的影响. 西北农林科技大学学报(自然科学版). 2016(02): 15-22 .
    15. 胡田恩，王玲，张春晓，宋凯，李金宝. 饲料中添加胆汁酸对牛蛙生长性能、体组成和营养物质表观消化率的影响. 水生生物学报. 2015(04): 677-685 . 本站查看
    16. 张晓雁，杜浩，危起伟，张艳珍，王彦鹏，蔡经江，刘志刚，乔新美，熊伟. 养殖中华鲟的产后康复. 水生生物学报. 2015(04): 705-713 . 本站查看
    17. 李剑，刘淑兰，史庆超，翟少伟. 饲料中添加槲皮素对吉富罗非鱼幼鱼生长性能、血清生化指标和非特异性免疫指标的影响. 饲料工业. 2014(16): 21-25 .
    18. 范兴，陆玉芹，陈名帅，颜明月，陈德慰. 三卡因甲磺酸盐麻醉对罗非鱼离水保活的影响. 食品科技. 2014(09): 174-179 .

    Other cited types(16)

Catalog

    Get Citation
    PDF
    XML
    Article views (945) PDF downloads (643) Cited by(34)
    Related

    Editorial Office of Acta Hydrobiologica Sinica

    Address：No. 7 Donghu South Road, Wuchang District, Wuhan, Hubei Province, China

    Tel：027-68780701；027-68780800 E-mail：acta@ihb.ac.cn  

    Supported byBeijing Renhe Information Technology Co. Ltd  Technical support：info@rhhz.net

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return