INTRODUCTION OF FOREIGN GENE CARRIED BY SPERMS

Mature eggs from carp (Cyprinus carpio L.)and loach (Misgurnus anguillicaudatus Cantor)were inseminated with the same species sperms which had been incubated withrecombinant plasmid DNA containing human GH gene in a solution containing 2.9%g lucose,1% sodium citrate,0.2% sodium bicarbonate,0.03% potassium chloride and 30mg/ml bovine albumin.Individuals of 33.3% carp fry and 37.0% loach fry showed tocarry the foreign gene with 4-150 copies per cell by dot blot hybridization and PCR analysis.The studies indicated that sperm could be a vector to carry and introduce foreign gene intofish eggs in the course of fertilization.