Volume 30 Issue 5
Aug.  2014
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HAN XingMei, LI YuanGuang, WEI XiaoDong, SUN YongRu, WANG YiQin. OPTIMIZATIONOF HETEROTROPHICMEDIUM FOR CULTURING TRANSGENIC CHLORELLA WITH RABBIT DEFENSIN GENE[J]. ACTA HYDROBIOLOGICA SINICA, 2006, 30(5): 547-552.
Citation: HAN XingMei, LI YuanGuang, WEI XiaoDong, SUN YongRu, WANG YiQin. OPTIMIZATIONOF HETEROTROPHICMEDIUM FOR CULTURING TRANSGENIC CHLORELLA WITH RABBIT DEFENSIN GENE[J]. ACTA HYDROBIOLOGICA SINICA, 2006, 30(5): 547-552.
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OPTIMIZATIONOF HETEROTROPHICMEDIUM FOR CULTURING TRANSGENIC CHLORELLA WITH RABBIT DEFENSIN GENE

  • Institute of Marine Bioprocess Engineering, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237;
    2. Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101

  • Received Date: August 02, 2005
  • Rev Recd Date: January 19, 2006
  • Published Date: September 24, 2006
    Graphical Abstract
    • Abstract
  • Based on the experiment of screening nitrogen sources, mixotrophicKnop mediumwas optimized by using of Plackett-Burman experimental design and the one-at-a-time strategy and the cell density of transgenic Chlorella was improved from 1. 12g/ L to 5. 11g/Lin 250mLflasks. Using the optimized medium, the cell density of the transgenic Chlorella in 250 mL flasks and 5L bioreactorwere 3. 39 and 3. 17 fold respectivelywhat can beobtained in themixotrophic Knop mediumwhile the expression capa-bilities of NP-1 were kept unchanged.
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    • References (12)
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