Volume 33 Issue 3
Aug.  2014
Turn off MathJax
Article Contents
Abstract
References
QIN Shan, LIU GuoXiang, HU ZhengYu. THE ACCUMULATION OF ASTAXANTHIN AND THE RESPONSE OF PHOTOSYNTHETIC ACTIVITY IN SCENEDESMUS OBLIQUUS[J]. ACTA HYDROBIOLOGICA SINICA, 2009, 33(3): 509-515.
Citation: QIN Shan, LIU GuoXiang, HU ZhengYu. THE ACCUMULATION OF ASTAXANTHIN AND THE RESPONSE OF PHOTOSYNTHETIC ACTIVITY IN SCENEDESMUS OBLIQUUS[J]. ACTA HYDROBIOLOGICA SINICA, 2009, 33(3): 509-515.
shu

THE ACCUMULATION OF ASTAXANTHIN AND THE RESPONSE OF PHOTOSYNTHETIC ACTIVITY IN SCENEDESMUS OBLIQUUS

  • 1.

    Institute of Hydrobiology,Chinese Academy of Sciences, Wuhan 430072;
    2. Graduate School of the Chinese Academy of Sciences, Beijing 100039

  • 1.

    Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan430072;
    2. Graduate University of Chinese Academy of Sciences, Beijing100039

  • Received Date: August 23, 2007
  • Rev Recd Date: December 12, 2008
  • Published Date: May 24, 2009
    Graphical Abstract
    • Abstract
  • The accumulation of astaxanthin in Scenedesmus obliquus under stress conditions was analyzed, and the respon-ses of photosynthetic activity and morphological change of algal cells were observed in the study. Under the temperature of content of carotenoid rose up from 0.25 mg/L to 0.44 mg/L in 48 hours. The composition of individual carotenoid was iso-lated and identified by HPLC/MS analysis. The results showed cells accumulated secondary carotenoids such as echinenone, adonixanthin, canthaxanthin, adonirubin and 3'-hydroxyechinenone and so on, the ketocarotenoid astaxan-thin (3,3'-dihydroxy-β, β-carotene-4,4'-dione) was found as a final product for the synthesis of secondary carotenoid. With the accumulation of secondary carotenoids, the algal coenobium composed of 4 or 8 cells was split up into single or two cells, and the shape of cells changed into swollen and irregular contrast to their initial state. The photosynthetic activity was also influenced by the stress conditions. The photosynthetic rate decreased about 50% in the first 3 hours, and then went up from 19.54 μmol O2/mg Chla/h to 34.29 μmol O2/mg Chlα/h in the next 9 hours. From 12 hours to 48 hours, the photosynthetic rate experienced a dramatically drop and reduced to nearly 5.21μmol O2/mg Chlα/h. The respiration rate of algal cells showed an inverse trend, which increased from 18.24μmol O2/mg Chlα/h to 60.37μmol O2/mg Chlα/h in the first 24 hours although there was a fluctuation in this course, then it decreased to 38.40μmol O2/mg Chlα/h in the next 24 hours which was still more higher than that of the control group. The change of chlorophyll fluores-cence tended to be similar to that of photosynthetic rate and decreased by 63.9%. These results indicated that the S. obliquus cells could biosynthesize astaxanthin by induced conditions. The accumulation of secondary carotenoids led the changes of contents ratio for chlorophyll to carotenoid. The light inhibition, enhanced respiration rate and the damage to PS Ⅱ were all responses to the stress, which also yielded more metabolism products and reactive oxygen species which further engendered the biosynthesis of secondary carotenoids. Simultaneously, stress conditions inhibited the cell division and led the changes of cell morphology. The regulating of photosynthetic activity and carotenoids accumulation were both the phsiol-igical mechanism for algal cells to resist the inclement environmental conditions.
    • FullText(HTML)
    • References (31)
  • [1]
    Johnson E A,An G H.Astaxanthin from microbial sources[J].CRC Critical Reviews in Biotechnology,1991,11:297-326
    [2]
    Miki W.Biological functions and activities of animal carotenoids[J].Pure and Applied Chemistry,1991,63:141-146
    [3]
    Tanaka T,Makita H,Ohnishi M,et al.Chemoprevention of rat oral carcinogenesis by naturally occurring xanthophylls,astaxanthin and canthaxanthin[J].Cancer Research,1995,55:4059-4064
    [4]
    Grung M,D' Souza F M L,Burowitzka M,et al.Algal carotenoids 51.Secondary carotenoids 2.Haematococcus pluvialis aphanospores as a source of (3S,3'S)-estaxanthin esters[J].Journal of Applied Phycology,1992,4:165-171
    [5]
    Brown T E,Richardson F L,Vaughn M L.Development of red pigmentation in Chlorococcum wimmeri (Chlorophyta:Chlorococcales)[J].Phycologia,1967,6:167-184
    [6]
    Grung M,Metzger P,Liaaen-jensen S.Algal carotenoids 42.Secondary carotenoids of algae 1.Primary and secondary carotenoids in two races of the green alga Botryococcus braunii[J].Biochemical Systematics and Ecology,1989,17:263-269
    [7]
    Rise M,Cohen E,Vishkantsan M,et al.Accumulation of secondary carotenoids in Chlorella Zofingiensis[J].Journal of Plant Physiology,1994,144:287-292
    [8]
    Yuan J P,Chen F,Liu X,et al.Carotenoid composition in the green microalga Chlorococcum[J].Food Chemistry,2002,76:319-325
    [9]
    Tjahjono A E,Hayama Y,Kakizono T,et al.Hyper-accumulation of astaxanthin in a green alga Haematococcus pluvialis at elevated temperatures[J].Biotechnology Letters,1994,16:133-138
    [10]
    Kohayashi M,Kakizono T,Nishio N,et al.Effects of light intensity,light quality,and illumination cycle on astaxanthin formation in a green alga,Haematococcus plavialis[J].Journal of Bioscience and Bioengineering,1992,74:61-63 [11] Masoj'dek J,Torzillo G,Kopecky' J,et al.Changes in chlorophyll fluorescence quenching and pigment composition in the green alga Chlorococcum sp.grown under nitrogen deficiency and salinity stress[J].Journal of Applied Phycology,2000,12:417-426
    [12]
    Kuang Q J,Zhao W Y,Deng P.Studies on the toxic efficiency of dibutylphthyalate to Scenedesmus obiliquus and natural algae[J].Acta Hydrobiologica Sinica,2003,27(1):103-105[况琪军,赵文玉,邓萍.DBP对斜生栅藻及天然混合藻类致毒效应研究.水生生物学报,2003,27(1):103-105]
    [13]
    Rippka R,Deroelles J,Waterbury J B,et al.Genetic assignments,strain histories and properties of pure cultures of cyanobacteria[J].Journal of General Microbiology,1979,111:1-61
    [14]
    Lichtenthaler H K.Chlorophylls and carotenoids:pigments of photosynthetic biomembranes[J].Methods in Enzymology,1987,148:351-382
    [15]
    Yuan J P,Chen F.Purification of trans-astaxanthin from a highyielding astaxanthin ester-producing strain of the microalga Haematococcus pluvialis-assay on rainbow trout,Oncorhynchus mykiss,pigmentation in comparison with synthetic astaxanthin and canthaxanthin[J].Food Chemistry,2000,68:443-448
    [16]
    Yuan J P,Chen F.Hydrolysis kinetics of astaxanthin esters and stability of astaxanthin of Haematococcus pluvialis during saponification[J].Journal of Agriculture and Food Chemistry,1999,47:31-35
    [17]
    Zhao X M,Bi Y H,Qin S,et al.The response of cultivated Nostoc flagelliforme to salt stress[J].Acta Botanicu Boreali-Occidentalis Sinica,2005,25:2234-2239[赵学敏,毕永红,秦山,等.发菜细胞培养物对盐胁迫的响应.西北植物学报,2005,25:2234-2239]
    [18]
    Stirbet A,Govindjee,Strassor B J,et al.Chlorophyll α fluorescence induction in higher plants:modeling and numerical simulation[J].Journal of Theoretical Biology,1998,193:131-151
    [19]
    Chen L Z,Xie Z M,Li D H,et al.Recovery of photosynthetic activity of Microcoleus vaginatus after water loss and rehydration[J].Acta Hydrobiologica Sinica,2006,30 (4):404-407[陈兰洲,谢作明,李敦海,等.失水-吸水过程中微鞘藻光合活性的特性.水生生物学报,2006,30(4):404-407]
    [20]
    Liu B H,Lee Y K.Composition and biosynthetic pathways of carotenoids in the astaxanthin-producing green alga Chlorococcim sp.[J].Biotechnology Letters,1999,21:1007-1010
    [21]
    Lu K X,Jiang X M,Zhai X W.Biological Function and application of astaxanthin[J].Journal of Ningbo University (NSEE),2003,16:95-98[陆开形,蒋霞敏,翟兴文.虾青素的生物学功能及应用.宁波大学学报,2003,16:95-98]
    [22]
    Cordero B,Otero A,Patino M,et al.Astaxanthin production from the green alga Haematococcus pluvialis with different stress conditions[J].Biotechnology Letters,1996,18:213-218
    [23]
    Kobayashi M,Kakizono T,Nishio N,et al.Effects of light intensity,light quality,and illumination cycle on astaxanthin formation in a green alga,Haematococcus pluvialis[J].Journal of General Microbiology,1992,74:61-63
    [24]
    Huang J C,Chen F,Sandmann G..Stress-related differential expression of multiple β-carotene ketolase genes in the unicellular green alga Haematococcus pluvialis[J].Journal of Biotechnology,2006,122:176-185
    [25]
    Rise M,Cohen E,Vishkautsan M,et al.Accumulation of secondary carotenoids in Chlorella Zofiagiensis[J].Journal of Plant Physiology,1994,144:287-292
    [26]
    Hagen C,Braune W,Bjorn LO.Functional aspects of secondary carotenoids in Haemntococcus lacustris (volvacales). Ⅲ.Action as a "sunshade"[J].Journal of Phycology,1994,30:241-248
    [27]
    Katsuya A,Hiroaki H,Morio H.Accumulation and antioxidant activity of secondary carotenoids in the aerial microalgs Coelastrella striolata var.multistrlata[J].Food chemistry,2007,100:656-661
    [28]
    Boussiba S.Carotenogenesis in the green alga Haematococcus pluvlalis:cellular physiology and stress response[J].Physiologia Plantarum,2000,108:111-117
    [29]
    Boussiba S,Vonshak A.Astaxanthin accumulation in the green alga Haematococcus plavlalis[J].Plant Cell Physiology,1991,32:1077-1082
    [30]
    Hagen C,Bornman G,Braune W.Reversible lowering of modulated chlorophyll fluorescence after saturating flashes in Haemato coccus lacustris (Volvocales) at room temperature[J].Physiologia Plantarum,1992,86:593-599
    [31]
    Zlotnik I S,Sukenik A,Dubinsky Z.Physiological and photosynthetic changes during the formation of red aplanospores in the chlorophyte Haematococcus pluvials[J].Journal of Phycology,1993,29:463-469
    [32]
    Tan S,Cunningham Jr F X,Youmans M,et al.Cytochrome f loss in astaxanthin accumulating red cells of Haematococcus phtvialis(Chlorophyeeac):Comparison of photosynthetic activity,photosynthetic enzymes and thylakoid membrane polypeptidos in red and green cells[J].Journal of Phycology,1995,31:897-905
    • Related Articles

Catalog

    Get Citation
    PDF
    XML
    Article views PDF downloads Cited by()
    Related

    Editorial Office of Acta Hydrobiologica Sinica

    Address：No. 7 Donghu South Road, Wuchang District, Wuhan, Hubei Province, China

    Tel：027-68780701；027-68780800 E-mail：acta@ihb.ac.cn  

    Supported byBeijing Renhe Information Technology Co. Ltd  Technical support：info@rhhz.net

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return