GENETIC DIVERSITY ANALYSIS OF CHINESE THREE-KEELED POND TURTLE (CHINEMYS REEVESII) BY RAPD

ZHU Xin-Ping; DU He-Jun; ZHOU Li; LI Ming-You; GUI Jian-Fang

Volume 29 Issue 2

Aug. 2014

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ACTA HYDROBIOLOGICA SINICA > 2005 > 29(2): 167-171.

ZHU Xin-Ping, DU He-Jun, ZHOU Li, LI Ming-You, GUI Jian-Fang. GENETIC DIVERSITY ANALYSIS OF CHINESE THREE-KEELED POND TURTLE (CHINEMYS REEVESII) BY RAPD[J]. ACTA HYDROBIOLOGICA SINICA, 2005, 29(2): 167-171.

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GENETIC DIVERSITY ANALYSIS OF CHINESE THREE-KEELED POND TURTLE (CHINEMYS REEVESII) BY RAPD

Institute of Hydrobiology, Chinese Academy of Sciences, Graduate school of the Chinese Academy of Sciences, Wuhan 430072;
2. Pearl River Fisheries Research Institute, Chinese Academy Fishery of Sciences, Guangzhou 510380

Received Date: December 16, 2003
Rev Recd Date: February 08, 2004
Published Date: March 24, 2005

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Abstract

Chinese three-keeled pond turtle (Chinemys reevesii Gray) is a member of the family Emydidae and widely ranges in southern China, especially in the regions of the lower and middle Yangtze River. It has high values for food, traditional Chinese medicine and pet trade, and is one of the turtles for aquaculture in China. But the genetic background of Chinese three-keeled pond turtle is still not very clear. In order to realize its population genetic structure and the diversity for the conservation of its germ plasm and prevention of gene loss, genetic diversity analysis was carried out using RAPD-PCR technique. In this paper, genetic diversity of 24 individuals of Chinese three-keeled pond turtle from Hubei province was analyzed by the random amplified polymorphic DNA (RAPD) technique, using 20 10-nucleotide-long primers. Twenty primers produced 3288 well-amplified DNA fragments with reproducible banding patterns and averaged 137 DNA fragments in one individual. 119 of 137 loci detected are polymorphic, amounting to 86.9%. The DNA fragments were from 0.2kb to 3kb. The genetic distance between two individuals was from 0.168 to 0.467. The average genetic distance was 0.324?.0631 among the 24 individuals. The phylogenetic tree of the 24 individuals was obtained by NJTREE analysis in the RAPDistance program and the 24 individuals were divided into several clusters and it indicated the turtle maybe have two populations at least. In conclusion, genetic diversity of Chinese three-keeled pond turtle was high.
- Chinese three-keeled pond turtle (Chinemys reevesii),
- Genetic diversity

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