FANG Zhi-Ping, PAN Qian-Sheng, LUO Li-Ting, WANG Xiang-Ling, PAN Yu-Shan. AN IMMUNOCYTOCHEMICAL LOCALIZATION OF GnRH AND GnRHR IN THE BRAIN,PITUITARY AND OVARY OFSILURUS ASOTUS ANDPELTEOBAGRUS FULVIDRACO IN MATURITY AND IMMA TURITY[J]. ACTA HYDROBIOLOGICA SINICA, 2004, 28(1): 63-68.
Citation: FANG Zhi-Ping, PAN Qian-Sheng, LUO Li-Ting, WANG Xiang-Ling, PAN Yu-Shan. AN IMMUNOCYTOCHEMICAL LOCALIZATION OF GnRH AND GnRHR IN THE BRAIN,PITUITARY AND OVARY OFSILURUS ASOTUS ANDPELTEOBAGRUS FULVIDRACO IN MATURITY AND IMMA TURITY[J]. ACTA HYDROBIOLOGICA SINICA, 2004, 28(1): 63-68.

AN IMMUNOCYTOCHEMICAL LOCALIZATION OF GnRH AND GnRHR IN THE BRAIN,PITUITARY AND OVARY OFSILURUS ASOTUS ANDPELTEOBAGRUS FULVIDRACO IN MATURITY AND IMMA TURITY

  • Received Date: January 22, 2003
  • Rev Recd Date: March 19, 2003
  • Published Date: January 24, 2004
  • Using the Strept Avidin Biotin-peroxidase Comple x(S. ABC) immunocytochemical staining technique,the immunoreactive endocrine cells in the brain,pituitary gland and ovary of oriental sheatfish(S. asotus) and ye llow catfish(P.fulvidraco) in maturity and immaturity were locatized with 2 kinds of antisera-gonadotropin releasing hormone(GnRH) and gonadotropin releasing hormone receptor(GnRHR).The result showed that there were GnRH and GnRHR immu noreactive endocrine cells in the brain,pituitary gland and ovary ofS. asotus andP. fulvidraco.The GnRH and GnRHR immunoreactive cells are more and th e immunoreactive intensity is higher in the hypothalamus,pituitary and ovary of both fish in maturity than in immaturity.There are no change of GnRH and GnRHR positive reactive substance in the telencephalon,midbrain,spinal bulb and cerebellum in maturity and immaturity.The positive neuron and neurofibrae are more in th e hypothalamus of both fish in maturity than in immaturity. GnRH immunoreactive neuron show dark brown in color and their cell nucleus are light color.The shape of GnRH immunoreactive neuron is variform,they show shuttle,oval and triangle i n shape.Most of the have one or two long cytoplasmic processes.The GnRH immunore active neurofibrae are dark brown in color and in the shape of string beads.This paper discussed that the possible physiological function and morphological evid ence of GnRH and GnRHR regulated directly or indirectly the development of gonad on S. asotusandP. fulvidraco.It provided new morphological basis for the research field of hypothalamus-pituitary-gonad axis,neuro-endocrine and th e functional diversity of GnRH.
  • [1]
    Gu Z P,Sang G W,Chen J K.Pharmacology of generation[M].Hefei:Anhui Education publishing House,1990,1-48[顾芝萍,桑国卫,陈俊康.生殖药理学.合肥:安徽教育出版社,1990,1-48][2] Yoav G,Abigail E,Marguerite C,Thomas T C,et al.Molecular cloning and charaterization of a novel gonadotropin-releasing hormone from the gilthead seabream [J].Molecular Marine Bioiogy and Biotechnology,1995,4(1):27-35[3] Stacia A S,Masumi N,Christopher J K,et al.The occurrence and distribulation of GnRH in the brain of Atlantic hagfish,an agnatha,determined by chromatography and immunocytochemistry [J].General and comparative endocrinology,1995,97:300-307[4] Jeferey I G,Rafe G,Christopher J P,et al. Presence and biological activity of a GnRH-like factor in the nervous system of Helisoma trivovis [J].The Journal of comparative neurology,1993,336:571-582[5] Claytoy R N,Catt K J.Gonadotropin-releasing hormone receptor:charaterization, physiological regulation,and relationship to reproductive function [J].Endocr Rev,1981,2:186[6] Amano M,Oka Y,Aida K.Immunohistochemical demonstation of salmon GnRH and chicken GnRH-Ⅱ in the brain of the masu salmon (Oncorhynchus masou) [J].The Journal of comparative neurology,1991,314:587-597[7] Fang Y Q,Huang W Q,Chen L.Distribution of gonadotropin-releasing hormone in the brain and Hatschek's pit of amphioxus (Branchiostoma belcheri) [J].Acta Zool.Sin.,1999a,45(1):106-111[方永强,黄威权,陈蕾.GnRH在文昌鱼脑和哈氏窝的分布.动物学报,1999a,45(1):106-111][8] Fang Y Q,Huang W Q,Chen L.Immunohistochemical localization of gonadotropin-releasing hormone receptors (GnRHR) in the nervous system, Hatschek's pit and gonads of Amphioxus,Branchiostoma belcheri [J].Chinese Science Bulletin,1999c,44(4):385-388[方永强,黄威权,陈蕾.GnRH受体在文昌鱼神经系统、哈氏窝和性腺的免疫组织化学定位.科学通报,1999c,44(4):385-388][9] Han X B,Cao Y Q,Zhuang L Z.GnRH receptor and the regulation of its gene exprission [J].Advance of Physiologic Science,1998,29(3):198-202[韩新兵,曹咏清,庄临之.促性腺激素释放激素受体及其基因表达的调控.生理科学进展,1998,29(3):198-202[10] Peter R E,Marchant T A.The endocrinology of growth in carp and related species [J].Aquaculture,1995,129:299-321[11] Jin X H and Huang W Q.Studying advances of immunoregulatory function of gonadotropin releasing hormone [J].Advance of Physiologic Science,2000,31(2):169-172[金晓航,黄威权.促性腺激素释放激素免疫调节作用的研究进展.生理科学进展,2000,31(2):169-172][12] Sherwood N M,Lovejoy D A,Coe I R.Origin of gonadotropin-releasing hormone [J].Endocr Res,1993,14:241-254[13] Rosenblum P M.Goos H J Th,Peter R E.Regional distribution and in vitro secretion of salmon and chicken-Ⅱ Gonadotropin-releasing hormone fron the brain and pituitary of juvenile and adult goldfish,Carassius auratu [J].General and comparative endocrinology,1994,93:369-379[14] Wang L and Lin H R.Distributions and variations of sGnRH in discrete brain areas from common carp (Cyprinus carpio) of different ages and gonadal conditions [J].Zoological Research,1998,19(3):197-202[王黎,林浩然.不同年龄和性腺发育期鲤鱼不同脑区的sGnRH分布及变化.动物学研究,1998,19(3):197-202][15] Masafumi A,Katsumi A,Naoto Q,Yoshihisa H.Changes in levels of GnRH in the brain and pituitary and GTH in the pituitary in male masu salmon,Oncorhynchus masou,from hatching to maturation [J].Fish Physiology and Biochemistry,1993,11:233-240

    Gu Z P,Sang G W,Chen J K.Pharmacology of generation[M].Hefei:Anhui Education publishing House,1990,1-48[顾芝萍,桑国卫,陈俊康.生殖药理学.合肥:安徽教育出版社,1990,1-48][2] Yoav G,Abigail E,Marguerite C,Thomas T C,et al.Molecular cloning and charaterization of a novel gonadotropin-releasing hormone from the gilthead seabream [J].Molecular Marine Bioiogy and Biotechnology,1995,4(1):27-35[3] Stacia A S,Masumi N,Christopher J K,et al.The occurrence and distribulation of GnRH in the brain of Atlantic hagfish,an agnatha,determined by chromatography and immunocytochemistry [J].General and comparative endocrinology,1995,97:300-307[4] Jeferey I G,Rafe G,Christopher J P,et al. Presence and biological activity of a GnRH-like factor in the nervous system of Helisoma trivovis [J].The Journal of comparative neurology,1993,336:571-582[5] Claytoy R N,Catt K J.Gonadotropin-releasing hormone receptor:charaterization, physiological regulation,and relationship to reproductive function [J].Endocr Rev,1981,2:186[6] Amano M,Oka Y,Aida K.Immunohistochemical demonstation of salmon GnRH and chicken GnRH-Ⅱ in the brain of the masu salmon (Oncorhynchus masou) [J].The Journal of comparative neurology,1991,314:587-597[7] Fang Y Q,Huang W Q,Chen L.Distribution of gonadotropin-releasing hormone in the brain and Hatschek's pit of amphioxus (Branchiostoma belcheri) [J].Acta Zool.Sin.,1999a,45(1):106-111[方永强,黄威权,陈蕾.GnRH在文昌鱼脑和哈氏窝的分布.动物学报,1999a,45(1):106-111][8] Fang Y Q,Huang W Q,Chen L.Immunohistochemical localization of gonadotropin-releasing hormone receptors (GnRHR) in the nervous system, Hatschek's pit and gonads of Amphioxus,Branchiostoma belcheri [J].Chinese Science Bulletin,1999c,44(4):385-388[方永强,黄威权,陈蕾.GnRH受体在文昌鱼神经系统、哈氏窝和性腺的免疫组织化学定位.科学通报,1999c,44(4):385-388][9] Han X B,Cao Y Q,Zhuang L Z.GnRH receptor and the regulation of its gene exprission [J].Advance of Physiologic Science,1998,29(3):198-202[韩新兵,曹咏清,庄临之.促性腺激素释放激素受体及其基因表达的调控.生理科学进展,1998,29(3):198-202[10] Peter R E,Marchant T A.The endocrinology of growth in carp and related species [J].Aquaculture,1995,129:299-321[11] Jin X H and Huang W Q.Studying advances of immunoregulatory function of gonadotropin releasing hormone [J].Advance of Physiologic Science,2000,31(2):169-172[金晓航,黄威权.促性腺激素释放激素免疫调节作用的研究进展.生理科学进展,2000,31(2):169-172][12] Sherwood N M,Lovejoy D A,Coe I R.Origin of gonadotropin-releasing hormone [J].Endocr Res,1993,14:241-254[13] Rosenblum P M.Goos H J Th,Peter R E.Regional distribution and in vitro secretion of salmon and chicken-Ⅱ Gonadotropin-releasing hormone fron the brain and pituitary of juvenile and adult goldfish,Carassius auratu [J].General and comparative endocrinology,1994,93:369-379[14] Wang L and Lin H R.Distributions and variations of sGnRH in discrete brain areas from common carp (Cyprinus carpio) of different ages and gonadal conditions [J].Zoological Research,1998,19(3):197-202[王黎,林浩然.不同年龄和性腺发育期鲤鱼不同脑区的sGnRH分布及变化.动物学研究,1998,19(3):197-202][15] Masafumi A,Katsumi A,Naoto Q,Yoshihisa H.Changes in levels of GnRH in the brain and pituitary and GTH in the pituitary in male masu salmon,Oncorhynchus masou,from hatching to maturation [J].Fish Physiology and Biochemistry,1993,11:233-240

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