CHEN Jin-Hui, HUANG Ming-Min, ZHENG Kang, LIN Kai-Dong, LUO Chen. RAPD ANALYSIS ON GENOMIC DNA OF TWO ARTIFICIAL GYNOGENETIC GROUPS OF GRASS CARP,CTENOPHARYNGODON IDELLU[J]. ACTA HYDROBIOLOGICA SINICA, 2004, 28(5): 471-477.
Citation: CHEN Jin-Hui, HUANG Ming-Min, ZHENG Kang, LIN Kai-Dong, LUO Chen. RAPD ANALYSIS ON GENOMIC DNA OF TWO ARTIFICIAL GYNOGENETIC GROUPS OF GRASS CARP,CTENOPHARYNGODON IDELLU[J]. ACTA HYDROBIOLOGICA SINICA, 2004, 28(5): 471-477.

RAPD ANALYSIS ON GENOMIC DNA OF TWO ARTIFICIAL GYNOGENETIC GROUPS OF GRASS CARP,CTENOPHARYNGODON IDELLU

  • Received Date: April 27, 2003
  • Rev Recd Date: November 19, 2003
  • Published Date: September 24, 2004
  • Genetic similarity,genetic distance and Shannon's genetic diversity of two different artificial gynogenetic grass carp groups and a control common grass carp group were analyzed in the methods of RAPD (Random Amplified Polymorphism DNA).One of the two different gynogenetic groups was a meiotic-mito-gynogenetic grass carp group that was produced by artificial gynogenesis for two generations. Another.gynogenetic group was a mito-gynogenetic grass carp that was generated by artificial gynogenesis only one generation.The common grass carp group was randomly collected from the markets in Changsha. Total 291 loci were detected in all of the three groups through the RAPD-PCR with 26 polymorphic random primers. The loci number in the three groups,meio- mito-gynogenetic group,the mito-gynogenetic group and the common grass carp group,were 265,272 and 282 and the number of polymorphic loci were 15,19 and 81 respectively. Genetic statistical analysis showed that percentage of polymorphic loci of the three groups of grass carp,were 5.66%,6.99%,28.23%;and the genetic similarity within the three groups of grass carp estimated by Nei’s index were 0.9851,0.9820,0.9114,respectively. The genetic diversity indices within the three groups of grass carp estimated by Shannon index of phenotypic diversity were 0.1722,0.3169 and 0.8450 respectively. Generally,the genetic diversity within two artificial gynogenetic groups of grass carp is far lower than that of common grass carp,while the genetic diversity in meiotic-mito-gynogenetic grass carp group is much lower than that of mito-gynogenetic grass carp group. The relatively low level of genetic diversity in each of the two groups of gynogenetic grass carp revealed that the population purity in each of the two gynogenetic grass carp groups is higher than that of common grass carp group.
  • [1]
    Wu Q J,Chen R D,Ye Y Z.Investigation on the carp gynogenesis with reference to establishing a pure Line [J]. Acta Genetica Sinica, 1981,8(1):50-55. [吴清江,陈荣德,叶玉珍. 鲤鱼人工雌核发育及其作为建立近交系新途径的研究. 遗传学报,1981,8(1):50-55][2] Williams J G K,Kubelik A R,livak K J,et al. DNA polymorphism amplified by arbitrary primers are useful as genetic markers[J]. Nucleic Acids Research,1990,18:6531-6535[3] Welsh J,McClelland M,. Fingerprinting genomes using PCR with arbitrary primers. Nucleic Acids Res, 1990,18:7213-7218[4] Wu Q J, Gui J F. Fish Genetics and Breeding Engineering[M]. Shanghai:Shanghai Scientific Technical Publishes. 1999. [吴清江,桂建芳. 鱼类遗传育种工程. 上海:上海科学和技术出版社.1999][5] Zou S M, LI S F,Cai W Q,et al. Establishing gynogenetic groups of genetic improved Megalobrama amblycephala and its genetic analysis. [J]. Journal of Fisheries of China,2001,25(4):311-316.[邹署明,李思发,蔡完其,等.团头鲂良种雌核发育群体的建立及其遗传变异. 水产学报,2001,25(4):311-316][6] Lu R,Rank G H. Use of RAPD population genetic parameters in the alfalfa leafcutting bee,Megachile rotundata[J]. Genome,1996,39:63-70[7] Xia D Q, Cao Y, Wu T T, et al. Study on lineages of Protosalanx chinensis,Neosalanx taihuensis and N. oligodontis in Taihu Lake with RAPD technique[J]. Journal of Fisheries of China,2000,7(1):12-15.[夏德全,曹莹,吴婷婷,等. 用RAPD方法分析太湖大银鱼、太湖新银鱼和寡齿新银鱼的亲缘关系. 中国水产科学,2000,7(1):12-15][8] Marillia E F,Scoles G J. The use of RAPD markers inHordeum Phylogeny [J].Genome,1996,39:646-654[9] Sun X W,Liang L Q. A genetic linkage map of common carp [J]. Journal of Fishery Sciences of China,2000,7(1):1-5[孙效文,梁利群. 鲤鱼的遗传连锁图谱.中国水产科学,2000,7(1):1-5][10] Lin J Z,Ritland K. Construction of a genetic linkage map in the wild plant Mimulus using RAPD and isozyme markers[J]. Genome,1996,39:63-70[11] Zhou L,Fan L C, Gui J F. RAPD analysis of incorporation of heterologousgenetic materisls in multiple species of Silver Crucian carp[J]. Acta Hydrobiologica Sinica,1998,22(4):301-303. [周莉,樊连春,桂建芳. 银鲫复合种外源遗传物质整入的RAPD分析. 水生生物学报,1998,22(4):301-303][12] Iturra P,Medrano J F, Bagley M. Identification of sex chromosome molecular markers using RAPD sand fluorescentin situ hybridization in rainbow trout[J]. Genetica,1998,101:209-213[13] Zhang S M, Deng H, Wang D Q et al population structure and diversity of silver carp and grass carp from populations of Yangtze River system revealed by RAPD[J]. Acta Hydrobiologica Sinica, 2001,25(4):324-330. [张四明,邓怀,汪登强,等.长江水系鲢和草鱼遗传结构及变异性的RAPD分析. 水生生物学报,2001,25(4):324-330][14] Zhang H Y, Liu R Z, Zhang X W, et al. Assessment of population genetic variation of grass carp and common carp using RAPD finger prints[J]. Acta hydrobiologica sinica, 1998,22(2):168-173.[章怀云,刘荣宗,张大明,等.草鱼和鲤鱼群体遗传变异的RAPD指纹分析.水生生物学报,1998,22(2):168-173][15] Stanley J G.Morphology of androgenetic and gynogenetic grass carp,Ctenopharyngodon idella(Valenciennes)[J].J. Fish Biol,1976,9:523-528[16] Luo C,Liu Y.Studies on production of gynogenetics grass carp and crucian carp[J]. Acta Sci Univ Norm Hunan,1991,14(2):154-159.[罗琛,刘筠.人工诱导草鱼和鲫鱼雌核发育的研究.湖南师范大学自然科学学报,1991,14(2):154-159][17] Li B X,Luo C.Cytological observations on induction of mitogynogenesis by heat-shocking of the eggs in grass carp,Ctenopharyngodon idellu[J].Acta Hydrobiologica Sinic,2003,27(2):155-160. [李冰霞,罗琛.热休克法抑制第一次卵裂实现草鱼雌核发育的细胞学观察[J].水生生物学报,2003,27(2):155-160][18] Tajima F. Evolutionary relationship of DNA sequence in finite populations[J]. Genetics,1983,105:437-445[19] Huang M M,Chen J H,Luo C et al.screening of polymorphic primers and polymorphic locus for grass carp genomic RAPD analysis[J].Science Technology and Engineering,2004,4(2):91~96[黄明敏,陈金辉,罗琛等.草鱼基因组随机引物扩增多态性引物及多态位点的筛选.科学技术与工程,2004,4(2):91~96][20] Dieffenbach C W,Dveksler G S.(translated by Huang P T,et al). PCR Primer:A Laboratory Manual[M]. Beijing:Science Press.2000.[C.W.迪芬巴赫,G.S.德维克斯勒著,(黄培堂等译). PCR技术实验指南[M].北京:科学出版社. 2000][21] Apostol B L,Black IV W C,Reiter P,et al. population genetics with RAPD-PCR markers:the breeding structure of Aedes aegypti in Puerto Rico [J]. Heredity,1996,76:325-334[22] Nei M.Li W H. Mathematical model for studying genetic variation in terms of restriction endonucleases[J].Proc. Natl. Acad. Sci. USA, 1979,76:5269-5273[23] Wachira F N, Waugh R, Hackett C A. et al. Detection on genetic diversity in tea (Camellia sinensis) using RAPD markers [J]. Genome,1995,38:201-210[24] Streisinger G, Walker C, Dower N, et al. Production of homozygous diploid zebra fish [J].Nature,1981,291:293-296[25] Kuhnlein U.Assessment of inbreeding by DNA fingerprinting:Development of a calibration curve using defined strains of chickens [J].Genetics,1990,125:161-165

    Wu Q J,Chen R D,Ye Y Z.Investigation on the carp gynogenesis with reference to establishing a pure Line [J]. Acta Genetica Sinica, 1981,8(1):50-55. [吴清江,陈荣德,叶玉珍. 鲤鱼人工雌核发育及其作为建立近交系新途径的研究. 遗传学报,1981,8(1):50-55][2] Williams J G K,Kubelik A R,livak K J,et al. DNA polymorphism amplified by arbitrary primers are useful as genetic markers[J]. Nucleic Acids Research,1990,18:6531-6535[3] Welsh J,McClelland M,. Fingerprinting genomes using PCR with arbitrary primers. Nucleic Acids Res, 1990,18:7213-7218[4] Wu Q J, Gui J F. Fish Genetics and Breeding Engineering[M]. Shanghai:Shanghai Scientific Technical Publishes. 1999. [吴清江,桂建芳. 鱼类遗传育种工程. 上海:上海科学和技术出版社.1999][5] Zou S M, LI S F,Cai W Q,et al. Establishing gynogenetic groups of genetic improved Megalobrama amblycephala and its genetic analysis. [J]. Journal of Fisheries of China,2001,25(4):311-316.[邹署明,李思发,蔡完其,等.团头鲂良种雌核发育群体的建立及其遗传变异. 水产学报,2001,25(4):311-316][6] Lu R,Rank G H. Use of RAPD population genetic parameters in the alfalfa leafcutting bee,Megachile rotundata[J]. Genome,1996,39:63-70[7] Xia D Q, Cao Y, Wu T T, et al. Study on lineages of Protosalanx chinensis,Neosalanx taihuensis and N. oligodontis in Taihu Lake with RAPD technique[J]. Journal of Fisheries of China,2000,7(1):12-15.[夏德全,曹莹,吴婷婷,等. 用RAPD方法分析太湖大银鱼、太湖新银鱼和寡齿新银鱼的亲缘关系. 中国水产科学,2000,7(1):12-15][8] Marillia E F,Scoles G J. The use of RAPD markers inHordeum Phylogeny [J].Genome,1996,39:646-654[9] Sun X W,Liang L Q. A genetic linkage map of common carp [J]. Journal of Fishery Sciences of China,2000,7(1):1-5[孙效文,梁利群. 鲤鱼的遗传连锁图谱.中国水产科学,2000,7(1):1-5][10] Lin J Z,Ritland K. Construction of a genetic linkage map in the wild plant Mimulus using RAPD and isozyme markers[J]. Genome,1996,39:63-70[11] Zhou L,Fan L C, Gui J F. RAPD analysis of incorporation of heterologousgenetic materisls in multiple species of Silver Crucian carp[J]. Acta Hydrobiologica Sinica,1998,22(4):301-303. [周莉,樊连春,桂建芳. 银鲫复合种外源遗传物质整入的RAPD分析. 水生生物学报,1998,22(4):301-303][12] Iturra P,Medrano J F, Bagley M. Identification of sex chromosome molecular markers using RAPD sand fluorescentin situ hybridization in rainbow trout[J]. Genetica,1998,101:209-213[13] Zhang S M, Deng H, Wang D Q et al population structure and diversity of silver carp and grass carp from populations of Yangtze River system revealed by RAPD[J]. Acta Hydrobiologica Sinica, 2001,25(4):324-330. [张四明,邓怀,汪登强,等.长江水系鲢和草鱼遗传结构及变异性的RAPD分析. 水生生物学报,2001,25(4):324-330][14] Zhang H Y, Liu R Z, Zhang X W, et al. Assessment of population genetic variation of grass carp and common carp using RAPD finger prints[J]. Acta hydrobiologica sinica, 1998,22(2):168-173.[章怀云,刘荣宗,张大明,等.草鱼和鲤鱼群体遗传变异的RAPD指纹分析.水生生物学报,1998,22(2):168-173][15] Stanley J G.Morphology of androgenetic and gynogenetic grass carp,Ctenopharyngodon idella(Valenciennes)[J].J. Fish Biol,1976,9:523-528[16] Luo C,Liu Y.Studies on production of gynogenetics grass carp and crucian carp[J]. Acta Sci Univ Norm Hunan,1991,14(2):154-159.[罗琛,刘筠.人工诱导草鱼和鲫鱼雌核发育的研究.湖南师范大学自然科学学报,1991,14(2):154-159][17] Li B X,Luo C.Cytological observations on induction of mitogynogenesis by heat-shocking of the eggs in grass carp,Ctenopharyngodon idellu[J].Acta Hydrobiologica Sinic,2003,27(2):155-160. [李冰霞,罗琛.热休克法抑制第一次卵裂实现草鱼雌核发育的细胞学观察[J].水生生物学报,2003,27(2):155-160][18] Tajima F. Evolutionary relationship of DNA sequence in finite populations[J]. Genetics,1983,105:437-445[19] Huang M M,Chen J H,Luo C et al.screening of polymorphic primers and polymorphic locus for grass carp genomic RAPD analysis[J].Science Technology and Engineering,2004,4(2):91~96[黄明敏,陈金辉,罗琛等.草鱼基因组随机引物扩增多态性引物及多态位点的筛选.科学技术与工程,2004,4(2):91~96][20] Dieffenbach C W,Dveksler G S.(translated by Huang P T,et al). PCR Primer:A Laboratory Manual[M]. Beijing:Science Press.2000.[C.W.迪芬巴赫,G.S.德维克斯勒著,(黄培堂等译). PCR技术实验指南[M].北京:科学出版社. 2000][21] Apostol B L,Black IV W C,Reiter P,et al. population genetics with RAPD-PCR markers:the breeding structure of Aedes aegypti in Puerto Rico [J]. Heredity,1996,76:325-334[22] Nei M.Li W H. Mathematical model for studying genetic variation in terms of restriction endonucleases[J].Proc. Natl. Acad. Sci. USA, 1979,76:5269-5273[23] Wachira F N, Waugh R, Hackett C A. et al. Detection on genetic diversity in tea (Camellia sinensis) using RAPD markers [J]. Genome,1995,38:201-210[24] Streisinger G, Walker C, Dower N, et al. Production of homozygous diploid zebra fish [J].Nature,1981,291:293-296[25] Kuhnlein U.Assessment of inbreeding by DNA fingerprinting:Development of a calibration curve using defined strains of chickens [J].Genetics,1990,125:161-165
  • Cited by

    Periodical cited type(6)

    1. 赵燕翘,连煜超,许文文,赵逸雪,韩高玲,赵洋. 中国人工蓝藻结皮研究进展. 中国沙漠. 2023(05): 214-222 .
    2. 李玉领,唐东山,李向阳,李敦海,刘凯旋,陈萌萌. 沙蒿胶-微藻联合固沙效果的试验研究. 工业安全与环保. 2018(03): 56-60 .
    3. 王建沅,周成旭,严小军,骆其君,蒋莹,马斌,谭应宏. 雨生红球藻在红光下的生长及营养盐消耗特征. 水生生物学报. 2014(06): 1135-1142 . 本站查看
    4. 叶功富,高伟,王亨,殷亮,黄石德,吴锡麟. 木麻黄光合生理特性对海岸线距离梯度的响应. 福建林学院学报. 2013(04): 291-297 .
    5. 董毅,吴统贵,顾建明,虞木奎,成向荣,段溪,王臣. 夹竹桃光合及荧光特性对海岸线距离的响应. 东北林业大学学报. 2012(12): 60-62+70 .
    6. 苏延桂,李新荣,陈应武,崔艳,鲁艳. 温度和CO_2浓度升高对荒漠藻结皮光合作用的影响. 应用生态学报. 2010(09): 2217-2222 .

    Other cited types(0)

Catalog

    Article views PDF downloads Cited by(6)
    Related

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return