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FANG Ping, LI Ying-Wen, HU Wei, DING Shi-Hua. CLONING AND SEQUENCE ANALYSIS OF cDNA ENCODING cGnRH-II FROM RICE FIELD EEL(MONOPTERUS ALBUS)[J]. ACTA HYDROBIOLOGICA SINICA, 2007, 31(2): 259-264.
Citation: FANG Ping, LI Ying-Wen, HU Wei, DING Shi-Hua. CLONING AND SEQUENCE ANALYSIS OF cDNA ENCODING cGnRH-II FROM RICE FIELD EEL(MONOPTERUS ALBUS)[J]. ACTA HYDROBIOLOGICA SINICA, 2007, 31(2): 259-264.
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CLONING AND SEQUENCE ANALYSIS OF cDNA ENCODING cGnRH-II FROM RICE FIELD EEL(MONOPTERUS ALBUS)

  • Chinese Academy of Fishery Sciences, Beijing 100039;
    2. College of Fisheries Science, Southwest Agriculture University, Chongqing 400716;
    3. The Key Laboratory of Animal Biology, Chongqing Normal University, Chongqing 400047;
    4. State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, the Chinese Academy of Sciences, Wuhan 430072

  • Received Date: March 14, 2005
  • Rev Recd Date: May 03, 2006
  • Published Date: March 24, 2007
    Graphical Abstract
    • Abstract
  • Gonadotropin-releasing hormone (GnRH) is a conserved nerval decapeptidethat regulates the development and maintenance of reproductive function in vertebrate1The cDNA encoding the [His5Trp7Tyr8] gonadotropin-releasing hormone ( chicken-?type GnRH, cGnRH-II) precursor from the brain of rice field eel ( Monopterus albus) has been isolated and sequenced by using reverse transcription and rapid amplification of cDNA ends ( RACE) 1From the subcloning and sequence analysis of 3c-RACE and 5c-RACE product, the complete sequence of the cDNA encoding its cGnRH-II peptide was identified and determined to be 617 bp in length1The structure of the encoded peptide is identical to all other cGnRH-II reported to date. The deduced peptide is composed of a signal peptide (SP), a GnRH decapeptide and a GnRH-associated peptide ( GAP) which is connected to GnRH by a Gly-Lys-Arg sequence1The cGnRH-II cDNA encoded SP of 21 amino acids and GAP of 49 amino acids. The amino acid sequence of cGnRH-II in combining with the Gly-Lys-Arg sequence is highly conserved during evolution when compared with the corresponding regions of other GnRH precursors. However, the untranslated region of GnRH are markedly divergent in nucleotide sequences
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    • References (28)
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