KUANG Xue-Mei, YE Yuan-Tu, CAI Chun-Fang, WANG Yong-Ling, JIANG-Rong. STUDY ON THE ABSORPTION AND UTILIZATION OF L -LEUCINE AND L -TYROSINE BY THE INTESTINE OF GRASS CARP (CTENOPHARYNGODON IDELLU S)IN VITRO[J]. ACTA HYDROBIOLOGICA SINICA, 2005, 29(4): 424-429.
Citation: KUANG Xue-Mei, YE Yuan-Tu, CAI Chun-Fang, WANG Yong-Ling, JIANG-Rong. STUDY ON THE ABSORPTION AND UTILIZATION OF L -LEUCINE AND L -TYROSINE BY THE INTESTINE OF GRASS CARP (CTENOPHARYNGODON IDELLU S)IN VITRO[J]. ACTA HYDROBIOLOGICA SINICA, 2005, 29(4): 424-429.

STUDY ON THE ABSORPTION AND UTILIZATION OF L -LEUCINE AND L -TYROSINE BY THE INTESTINE OF GRASS CARP (CTENOPHARYNGODON IDELLU S)IN VITRO

  • Received Date: May 16, 2004
  • Rev Recd Date: August 27, 2004
  • Published Date: July 24, 2005
  • The transmural fluxes of L-Leucine and L-Tyrosine were measured by in the intestinal gut, using the in vitro perfusiontechnique. The transmural rates of L-Leucine are01026, 01062 and 01038 (L mol/g/min)when the concentrations of the perfusionLeucine are 110, 510 and 1010mmol/L at the 10 min respectively. The transmural rates of L-Tyrosine are 01006, 01024 and01039 (Lmol/g/min) when the concentrations of the perfusion L-Tyrosine are 015, 115 and 215mmol/L the 10min respective-ly1The total absorption ratios of the L-Leucine are 12.83%, 18.85% and 16.45% when the concentrations of the perfusionL-Leucine are 110, 510 and 1010mmol/L for 40 minutes respectively. The total absorption ratios of the L-Tyrosine are 22.18%,23.68% and 27.49% when the concentrations of the perfusion L-Tyrosine are 0.5,1.5 and 2.5mmol/L for 40 minutes respec-tively.The percentage of the transmural fluxes through the intestinal gut, the free in intestine, that in new synthesized protein andthe remainders in intestine to the total absorbed amino acid were calculated respectively. When the concentration of the perfusionL-Leucine are changed from 1.0mmol/L to 5.0mmol/L and 10.0mmol/L, the transmural ratio are 83%,66% and 35% respec-tively. The free in intestine are 9%,28% and 49% respectively. That in protein are 2%, 5% and 13%. The remainders are6%,1% and 3% respectively. When the concentration of the perfusion L-Tyrosine are changed from 0.5mmol/L to 1.5mmol/Land 2.5mmol/L, the transmural ratio are 52%, 55% and 55% respectively. The free in intestine are 7%, 10% and 16% re-spectively. That in protein are 17%, 15% and 16%. The remainders are 24%, 20% and 13% respectively.
  • [1]
    Su N, Zhou A G. Intestinal mucosal amino acid catabolism and itsimplications[J]. Journal of Sichuan Institute of Animal Husbandryand Veterinary Medicine. 2000, 14(2): 47-52[苏宁, 周安国 肠粘膜中氨基酸的分解代谢意义. 四川畜牧兽医学院学报,2000, 14(2): 47-52]
    [2]
    Stoll B, Henry J, Reeds PJ., et al. Catabolism dominates the firs- tpass intestinal metabolism of dietary essential amino acids in milkprotein -fed piglets[J]. J Nutr 1998,128, 606-614
    [3]
    Ye Y T, Ling S M, Luo L,et al. Study on the Absorpition ofL -Leucine and L -Phenylalanine by the intestine of Grass carp(Ctenopharyngodon idellus -[J], Acta Zoologica Sinica.2000,46(1): 52-57[叶元土, 林仕梅, 罗莉, 等, 草鱼肠道对亮氨酸和苯丙氨酸的吸收. 动物学报, 2000,46(1): 52-57]
    [4]
    Ye Y T, Cai C F,Lin S M, et al.The Perfusion Technique of the FishIntestine in Vitro [J].China Animal Husbandry and VeterinaryMedicine,2002, 29(6):26-27[叶元土, 蔡春芳, 林仕梅, 等, 鱼类肠道离体灌流试验系统, 中国畜牧兽医,2002, 29(6):26-27]
    [5]
    Ye Y T,Ling SM,Luo L, et al. The Transmural Fluxes of 10 Ensen -tional Amino Acid By the Intestinal Gut of Grass Carp(Ctenopharyn -godon idellus-in Vitro [J]. Jouranl of Fishery Sciences of China,2003, 10(4): 311-317[叶元土, 林仕梅, 罗莉, 等, 草鱼肠道对10种必需氨基酸的吸收. 中国水产科学, 2003, 10(4): 311)317]
    [6]
    Wu G Y, Pan H Z, Wu H, Data Enchiridionof Biochemistry andbiomolecule[M], Beijing: Science Press, 2002, 112-113[吴冠芸,番华珍, 吴, 生物化学与分子生物学实验常用数据手册. 北京: 科学出版社, 2002, 112-113]
    [7]
    Windmueller H G. Glutamine utilization by the small intestine[J].Adv. Enzymol, 1982, 53: 201-237
  • Related Articles

    [1]CHEN Xiao-Qun, ZHOU Yan-Gen, JIA Kun-Tong, LIU Rong-Xin, YANG Xiao-Gang, GAO Qin-Feng, DONG Yun-Wei, DONG Shuang-Lin. COMPARATIVE STUDY ON GROWTH, BIOCHEMICAL INDEX, AND GENE EXPRESSION OF RAINBOW TROUT AND STEELHEAD UNDER CHRONIC HYPOXIA[J]. ACTA HYDROBIOLOGICA SINICA, 2024, 48(11): 1789-1800. DOI: 10.7541/2024.2024.0087
    [2]YAN Quan-Gen, ZHU Xiao-Ming, YANG Yun-Xia, HAN Dong, JIN Jun-Yan, XIE Shou-Qi, LI Zhong-Jie. EFFECT OF REPLACEMENT OF FISH MEAL WITH COTTONSEED MEAL ON GROWTH, HEMATOLOGICAL PHYSIOLOGY, AND BODY COMPOSITION OF GRASS CARP[J]. ACTA HYDROBIOLOGICA SINICA, 2014, 38(2): 362-369. DOI: 10.7541/2014.51
    [3]YUE Nan-Nan, WANG Mi, YAN Yu-Lian, LI Jian, LUO Qi-Yong, XIE Xiao-Jun. EFFECTS OF DIETARY PHE EXPOSURE ON THE HAEMATOLOGICAL INDEXES AND HEPATOPANCREAS OF SPINIBARBUS SINENSIS[J]. ACTA HYDROBIOLOGICA SINICA, 2014, 38(2): 304-311. DOI: 10.7541/2014.44
    [4]JIN Li, ZHAO Na, ZHOU Chuan-Jiang, HUANG Lin, ZHANG Yao-Guang. Effects of Starvation on Hematological Parameters and Hematogenesis in Myxocyprinus asiaticus[J]. ACTA HYDROBIOLOGICA SINICA, 2012, 36(4): 665-673. DOI: 10.3724/SP.J.1035.2012.00665
    [5]CHEN Yong-Xiang, XIAO Ling-Yuan, YAN Tai-Ming, ZHAO Hai-Tao, SHEN Shi-Jun, ZHOU Ding-Gang. HEMATOLOGICAL OFW ILD AND CULTURED SCHIZOTHORAC IN FISHES[J]. ACTA HYDROBIOLOGICA SINICA, 2009, 33(5): 905-910.
    [6]SHAO Yan, WU Zhi-Qiang, WANG Jian-Wei, CHANG Jian-Bo, CAO Wen-Xuan. STUDIES ON THE HAEMOTOLOGICAL INDICES OF GOBIOCYPRIS RARUS[J]. ACTA HYDROBIOLOGICA SINICA, 2006, 30(2): 232-235.
    [7]CHEN Gang, ZHOU Hui, ZHANG Jian-Dong, WU Zao-He. HEMATOLOGICAL STUDY AND OBSERVATION ON DEVELOPMENT OF BLOOD CELLS IN COBIA, RACHYCENTRON CANADUM[J]. ACTA HYDROBIOLOGICA SINICA, 2005, 29(5): 564-570.
    [8]LUO Yi-Ping, YUAN Lun-Qiang, CAO Zhen-Dong, XIE Xiao-Jun. THE STUDY ON HAEMATOLOGICAL PARAMETERS OF MYSTUS MACROPTERUS AND PELTEOBAGRUS VACHELLII IN JIALING RIVER[J]. ACTA HYDROBIOLOGICA SINICA, 2005, 29(2): 161-166.
    [9]Guan Zhimei, Chen Daoquan, Wang Xiaoqiang. A PRELIMINARY STUDY ON HEMATOLOGY OF BLACK FINLESS PORPOISE(NEOPHOCAENA PHOCAENOIDES)[J]. ACTA HYDROBIOLOGICA SINICA, 1989, 13(1): 76-82.
    [10]Zhao Mingji, Su Zegu, Huang Wenyu, Wang Zuxiong. ON THE HEMATOLOGICAL INDICES OF POND-REARED COMMON CARP AND GRASS CARP[J]. ACTA HYDROBIOLOGICA SINICA, 1979, 3(4): 453-464.

Catalog

    Article views (922) PDF downloads (481) Cited by()
    Related

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return