ZHANG Qing hua, LU Cheng ping. STARVATION-SURVIVAL AND RESUSCITATION OF GFP-MARKED Ah4332 IN THE OVERWINTERING WATER[J]. ACTA HYDROBIOLOGICA SINICA, 2002, 26(5): 465-471.
Citation: ZHANG Qing hua, LU Cheng ping. STARVATION-SURVIVAL AND RESUSCITATION OF GFP-MARKED Ah4332 IN THE OVERWINTERING WATER[J]. ACTA HYDROBIOLOGICA SINICA, 2002, 26(5): 465-471.

STARVATION-SURVIVAL AND RESUSCITATION OF GFP-MARKED Ah4332 IN THE OVERWINTERING WATER

  • Received Date: April 07, 2001
  • Rev Recd Date: June 05, 2001
  • Published Date: September 24, 2002
  • Starvation survival of GFP marked Ah4332 strain had been detected at 8~10℃ in the overwintering water in laboratory. The changes of bacterial amount were monitored by three counting ways.After 41 days the plate counts)PC)of Ah4332GFP dropped to zero. In all cases,the total number of Ah4332GFP remained the same level with direct viable count(DVC)or direct count(DC),but the DVC was about one or two log lower than the DC.It suggested that the Ah4332GFP had entered a dormant,viable but nonculturable(VBNC)state. The resuscitation of VBNC Ah4332GFP strain was tested in different conditions.Under increasing the temperature,culturable Ah4332GFP strain was recovered from VBNC state by two methods of either adding some substances e.g.fish serum,supernatant from late logarithmic phase Ah4332GFP cultures or injecting VBNC Ah4332GFP into the rabbit ligated ileal loop. By using electron microscopy and vertical fluorescent microscopy,the culturable and VBNC Ah4332GFP were observed.Comparing with the culturable cells of Ah4332GFP ,the VBNC bacteria processed a significant reduction in cell size and a morphological change from bacilli to cocci,meanwhile the cell membrane and cell wall were intact.It demonstrated that the VBNC state of Ah4332GFP was not the L-form bacterium.
  • [1]
    陆承平.致病性嗜水气单胞菌及其所致鱼病[J].水产学报,1992,16(3):282-288[2] Xu H-S.,Roberts N,Singleton FL el al.Survival and viability of nonculturable Escherichia coli and vibrio cholerae in the estuarine and marine environment.Microb Ecol[J],1982,8:313-323[3] 徐怀恕,Colwell RR.霍乱弧菌的越冬方式-活的非可培养状态及其检测方法 [J].青岛海洋大学学报,1989,19(2):77-83[4] Roszak DB,Grimes DJ,Colwell RR.Viable but nonculturable stage of Salmonella enteritidis in aquatic systems[J].Can J.Microbiol,1984,30(3):334-338[5] Morita RY.Bioavilability of energy and its relationship to growth a nd starvation survival in nature[J].Can J Microbial,1988,34(4):436-441[6] May PS,Pauling C,Morita RY.Starvation-survival processes of a mari ne Vibrio[J].Appl Environ Microbiol,1983a,45:1041-1048[7] May PS,Pauling C,Morita RY.Recovery from nutrient starvation by a m arine Vibrio sp.[J].Appl Environ Microbiol,1983b,45:1685-1690[8] Hartke A,Bouche S,Gansel X.Starvation-induced stress resistance in Lactococcus lactis subsp lactis IL 1403[J].Appl Environ Microbiol, 1994,60(9):3474-3478[9] Nilsson L,Oliver JD,Kjelleberg S.Resuscitation of Vibrio vulnific us from the viable but nonculturable state[J].J Bacterial,1991, 173(16):5054-5059[10] Ferguson Y,Glover LA,Mcgillivray DM at al.Survival and activity of l ux-marked Aeromonas salmonicida in seawater[J].Appl Environ Microbiol, 1995,61(9):3494-3498[11] Kogure K.A tentative direct microscopic method for counting living marin e bacteria[J].Can J Microbiol,1979,25:415-420[12] 许兵,徐怀恕,纪伟尚,等.活的非可培养状态霍乱弧菌的复苏[J]. 青岛海洋大学学报,1994,24(2):187-194[13] Spira WM.Simple adult rabbit model for vibrio cholerae and enterotox igenic Escherichia coli diarrhea[J].Infect Immun,1981,32:739-742[14] Siporin C.The evolution of fluorinated quinolones:pharmacology,microbiol ogical activity,clinical uses,and toxicities[J].Annu Rev Microbiol,1989,43:601-627[15] Quinn JP.The modification and evaluation of some sytochemical techniques for the enumeration of metabolically active heterotrophic bacteria in the aquat ic environment[J].J Appl Bacteriol,1984,57:51-57

    陆承平.致病性嗜水气单胞菌及其所致鱼病[J].水产学报,1992,16(3):282-288[2] Xu H-S.,Roberts N,Singleton FL el al.Survival and viability of nonculturable Escherichia coli and vibrio cholerae in the estuarine and marine environment.Microb Ecol[J],1982,8:313-323[3] 徐怀恕,Colwell RR.霍乱弧菌的越冬方式-活的非可培养状态及其检测方法 [J].青岛海洋大学学报,1989,19(2):77-83[4] Roszak DB,Grimes DJ,Colwell RR.Viable but nonculturable stage of Salmonella enteritidis in aquatic systems[J].Can J.Microbiol,1984,30(3):334-338[5] Morita RY.Bioavilability of energy and its relationship to growth a nd starvation survival in nature[J].Can J Microbial,1988,34(4):436-441[6] May PS,Pauling C,Morita RY.Starvation-survival processes of a mari ne Vibrio[J].Appl Environ Microbiol,1983a,45:1041-1048[7] May PS,Pauling C,Morita RY.Recovery from nutrient starvation by a m arine Vibrio sp.[J].Appl Environ Microbiol,1983b,45:1685-1690[8] Hartke A,Bouche S,Gansel X.Starvation-induced stress resistance in Lactococcus lactis subsp lactis IL 1403[J].Appl Environ Microbiol, 1994,60(9):3474-3478[9] Nilsson L,Oliver JD,Kjelleberg S.Resuscitation of Vibrio vulnific us from the viable but nonculturable state[J].J Bacterial,1991, 173(16):5054-5059[10] Ferguson Y,Glover LA,Mcgillivray DM at al.Survival and activity of l ux-marked Aeromonas salmonicida in seawater[J].Appl Environ Microbiol, 1995,61(9):3494-3498[11] Kogure K.A tentative direct microscopic method for counting living marin e bacteria[J].Can J Microbiol,1979,25:415-420[12] 许兵,徐怀恕,纪伟尚,等.活的非可培养状态霍乱弧菌的复苏[J]. 青岛海洋大学学报,1994,24(2):187-194[13] Spira WM.Simple adult rabbit model for vibrio cholerae and enterotox igenic Escherichia coli diarrhea[J].Infect Immun,1981,32:739-742[14] Siporin C.The evolution of fluorinated quinolones:pharmacology,microbiol ogical activity,clinical uses,and toxicities[J].Annu Rev Microbiol,1989,43:601-627[15] Quinn JP.The modification and evaluation of some sytochemical techniques for the enumeration of metabolically active heterotrophic bacteria in the aquat ic environment[J].J Appl Bacteriol,1984,57:51-57

Catalog

    Article views (833) PDF downloads (457) Cited by()
    Related

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return