Volume 32 Issue 1
Aug.  2014
Turn off MathJax
Article Contents
Abstract
References
WANG Hong-Xia, ZANG Xiao-Jun, LI Fu-Hua, XIANG Jian-Hai. KINSHIP ANALYSIS IN THE CHINESE SHRIMP ( FENNEROPENAEUS CHINENSIS) BASE ON MICROSATELLITE DNA MARKERS[J]. ACTA HYDROBIOLOGICA SINICA, 2008, 32(1): 42-46.
Citation: WANG Hong-Xia, ZANG Xiao-Jun, LI Fu-Hua, XIANG Jian-Hai. KINSHIP ANALYSIS IN THE CHINESE SHRIMP ( FENNEROPENAEUS CHINENSIS) BASE ON MICROSATELLITE DNA MARKERS[J]. ACTA HYDROBIOLOGICA SINICA, 2008, 32(1): 42-46.
shu

KINSHIP ANALYSIS IN THE CHINESE SHRIMP ( FENNEROPENAEUS CHINENSIS) BASE ON MICROSATELLITE DNA MARKERS

  • Experimental Marine Biology Laboratory, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071

  • Received Date: January 22, 2006
  • Rev Recd Date: January 10, 2007
  • Published Date: January 24, 2008
    Graphical Abstract
    • Abstract
  • The mating mode of Chinese shrimp ( F. chinensis) is supposed to be pair mating from observation of their matinghabits. However,this suggestion has not been confirmed genetically yet. In this study,five microsatellite loci ( Fc04, Fc06, Fc18,Fc24 and Fc27) were used to survey the mating mode of the wild Chinese shrimp in ten families which were built in differentyears. The male’s genotypes could be determined fromthose of a female and her offspring at every locus.Only one combination ofmale genotypes was observed in each family. Farther,the spermDNAof wild male was extracted from copulated wild female thelycum in three families of the ten. The result of microsatellite markers showed that the sperm DNA of wild males were not contami2nated by female genome DNA.At each locus,every progeny had one allele found in the male genotype. The distributions of geno2types in offspring of the ten families were consistent with the Mendelian segregation.All of these suggested that one female copu2lated to one male and supported that the mating mode of this prawn is pair2mating. The cluster analysis based on UPMGA (unweighted pair2group method using arithmetic averages) between individual within/ among families was also examined and showedthat each family formed single cluster,respectively.Microsatellite markers as an effective tool for kinship analysis could be widelyused to reveal the population structure,mating mode,etc. in breeding programs.
    • FullText(HTML)
    • References (18)
  • [1]
    Gao A B,Wu D J. Microsatellite markers for paternity testing ofLiangshan semifine wool sheep [J]. Hereditas (Beijing),2005,27(1):85-90 [高爱保,吴登俊. 利用微卫星标记进行凉山半细毛羊亲权鉴定的研究. 遗传,2005,27 (1):85-90]
    [2]
    Xu P,Zhou L H,Xiang J H. Isolating microsatellite DNA of Chineseshrimp Penaeus Chinensis [J]. Oceanologica et Limnologia Sinica,2001,32(3):255-259 [徐鹏,周岭华,相建海. 中国对虾微卫星DNA 的筛选. 海洋与湖沼,2001,32(3):255-259]
    [3]
    Liu P,Meng X H,He Y Y, et al. Genetic diversity in three wild pop2ulations of shrimp Fenneropenaeus Chinensis in Yellow and Bohaiseas as revealed by microsatellite DNA [J]. Oceanologica et Lim2nologia Sinica,2004,35 (3):252-256 [刘萍,孟宪红,何玉英,等. 中国对虾( Fenneropenaeus Chinensis) 黄、渤海3 个野生地理群体遗传多样性的微卫星DNA 分析. 海洋与湖沼,2004,35(3):252-256]
    [4]
    Zhang T S,Wang Q Y,Liu P, et al. Genetic diversity analysis on selected populations of shrimp Fenneropenaeus Chinensis by microsatel2lites [J]. Oceanologica et Limnologia Sinica,2005,36 (1):72-80[张天时,王清印,刘萍,等. 中国对虾( Fenneropenaeus Chinensis) 人工选育群体不同世代的微卫星分析. 海洋与湖沼,2005,36 (1):72-80]
    [5]
    Gao H X. Primary observation of Penaeus Chinensis behavior in mat2ing season [J]. Marine Sciences,1980,3:5-7 [高洪绪. 中国对虾交配期的初步观察. 海洋科学,1980,3:5-7]
    [6]
    Wang Q Y,Li J,Sun X T, et al. Studies on the mating ability andspermatophore regeneration in marine shrimp Penaeus Chinensis [J].Oceanologica et Limnologia Sinica,1998,29 (1):22-28 [王清印,李健,孙修涛,等. 中国对虾雄对虾交配能力和精荚再生的研究. 海洋与湖沼,1998,29(1):22-28]
    [7]
    Buresch KM,Hanlon R T,Maxwell M R, et al. Microsatellite DNAmarkers indicate a high frequency of multiple paternity within indi2vidual field-collecting egg capsules of the squid Loligo pealeii [J].Mar. Ecol. Prog. Ser.,2001,210:161-165
    [8]
    Paterson I G, Partridge V,Buckland2Nicks J. Multiple paternity inLittorina obtusata (Gastropoda,Littorinidae) revealed by microsatellite analysis [J]. Biol. Bull.,2001,20:261-267
    [9]
    Baragona M A,Haig-Ladewig L A,Wang S Y.Multiple paternity inthe grass shrimp Palaemonetes pugio [J]. Am. Zool.,2000,40:935
    [10]
    Ireland J S,Broderick A C,Glen F, et al.Multiple paternity assessedusing microsatellite markers,in green turtle Chelonia mydas (Lin2naeus, 1758) of Ascension Island, South Atlantic [J]. J. Exper.Mar. Biol. Ecol.,2003,291:149-160
    [11]
    Marshall T C,Slate J,Kruuk L. Statistical confidence for likelihood-based paternity inference in natural populations [J]. Mol. Ecol.,1998,7(5):639-655.
    [12]
    Jin L,Chakraborty R. Estimation of genetic distance and coefficient ofgene diversity from single2probe multilocus DNA fingerprinting data[J]. Mol. Biol. Evol.,1993,11 (1):120-127
    [13]
    Jones A G,Stockwell C A,Walker A, et al. The molecular basis of amicrosatellite null allele from the white sands pupfish [J]. J.Hered.,1999,89:339-342
    [14]
    Banks MA,Blouin M S,Baldwin B A, et al. Isolation and inheritanceof novel microsatellites in Chinook salmon ( Oncorhymchustschawyscha) [J]. J. Hered.,1999,90:281-288
    [16]
    Ball A O,Leonard S,Chapman R W. Characterization of ( GT),microsatellites from native white shrimp Penaeus setiferus [J]. Mol.Ecol.,1998,7:1251-1253
    [17]
    Wang H X,Wu C G,Zhang L S, et al. The application of microsatellite markers for parentage determination in selective breeding of pacific white shrimp ( Litopenaeus vannamei ) [J]. Hereditas (Bei-jing),2006,28:179-183 [王鸿霞,吴长功,张留所,等. 微卫星标记应用于凡纳滨对虾家系鉴别的研究. 遗传,28:179-183]
    [18]
    Wang H X,Li F H,Xiang J H. Polymorphic EST-SSR markers andtheir mode of inheritance in Fenneropenaeus chinensis [J]. Aquacul2ture,2005,249:107-114
    [19]
    Wu C G,Zhou L H,Xiang J H, et al. Morphology and Structure ofthelycum in Trachypenaeus Curvirostris and Penaeus Chinensis [J].Journal of Fisheries of China,1999,23(3):223-228 [吴长功,周令华,相建海,等. 鹰爪虾与中国对虾雌虾纳精囊的形态结构. 水产学报,1999,23(3):223-228]
    • Related Articles

  • Cited by

    Periodical cited type(6)

    1. 陈辰，滑少波，李杰勤，姜华，黄鹤平，姜婉琦，姚敏学，李向飞，初晓辉，刘秦华. 紫花苜蓿青贮饲料对草鱼生长性能和肌肉品质的影响. 草地学报. 2024(09): 2990-2998 .
    2. 李彤，司梦雅，崔青曼，袁春营. 紫花苜蓿对中华绒螯蟹生长性能、消化酶活性及代谢相关因子基因表达的影响. 饲料研究. 2023(11): 60-65 .
    3. 陈拥军，邹滔，林仕梅，罗莉，李云. 草鱼体组成的数学描述. 水产学报. 2016(04): 566-576 .
    4. 宋坚，何舟，程龙，庞云龙，宁军号，常亚青. 饲料添加苜蓿草粉对刺参幼参生长、体成分及免疫酶的影响. 草业学报. 2015(02): 208-214 .
    5. 宋盛亮，李君华，李静，刘佳亮，曹学彬，曲玺昌，王洪敏. 花生秧部分替代马尾藻对仿刺参生长、消化的影响. 水产科学. 2014(11): 685-691 .
    6. 李旭，章世元，陈四清，邹安革，高菲，孙慧玲，燕敬平，刘长琳. 四种饲料原料对刺参生长、体成分及消化生理的影响. 饲料工业. 2013(08): 36-40 .

    Other cited types(7)

Catalog

    Get Citation
    PDF
    XML
    Article views PDF downloads Cited by(13)
    Related

    Editorial Office of Acta Hydrobiologica Sinica

    Address：No. 7 Donghu South Road, Wuchang District, Wuhan, Hubei Province, China

    Tel：027-68780701；027-68780800 E-mail：acta@ihb.ac.cn  

    Supported byBeijing Renhe Information Technology Co. Ltd  Technical support：info@rhhz.net

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return