Volume 29 Issue 2
Aug.  2014
Turn off MathJax
Article Contents
Abstract
References
LUO Xiao-Chun, LI An-Xing, XIE Ming-Quan. STUDIES ON THE MORPHOLOGY OF GROUPER (EPINEPHELUS COIOIDES) MUCOSAL IMMUNE SYSTEM[J]. ACTA HYDROBIOLOGICA SINICA, 2005, 29(2): 198-193.
Citation: LUO Xiao-Chun, LI An-Xing, XIE Ming-Quan. STUDIES ON THE MORPHOLOGY OF GROUPER (EPINEPHELUS COIOIDES) MUCOSAL IMMUNE SYSTEM[J]. ACTA HYDROBIOLOGICA SINICA, 2005, 29(2): 198-193.
shu

STUDIES ON THE MORPHOLOGY OF GROUPER (EPINEPHELUS COIOIDES) MUCOSAL IMMUNE SYSTEM

  • School of Life Sciences, Sun Yat-sen University, Guangzhou 510275

  • Received Date: December 17, 2003
  • Rev Recd Date: November 17, 2004
  • Published Date: March 24, 2005
    Graphical Abstract
    • Abstract
  • Recent studies have shown fish mucosal immune system plays an important role in disease defense. In the present study,we examined the morphology of groupers (Epinephelus coioides) mucosal-associated immune tissues including skin,eye’s corn,gill,foregut and hindgut. The microstructures of these tissues were observed on the tissue sections using H-E staining,and the ultrastructures of immune cells were described using transmission electron microscopic technology. The results show several kinds of immune cells existing in mucosal-associated tissues including goblet cells,lymphocytes,macrophages,monocytes,eosinophils and nertrophils. There are many Goblet cells in skin epidermis,gill epithelial layer and lamina epithelialis,where they secret mucus to construct the first body defense line to pathogens intrusion. Lymphocytes are abundant in lamina propria of hindgut and gill sinusoids,and also observed in foregut lamina propria,basal layer of skin epidermis and between epidermis and dermis of skin. Lymphocyte’s distribution in mucosal tissues indicates mucus antibody may be mainly secreted by local ASCs (Antibody secreting cells). Phage cells and granules distribute similarly as lymphocytes and function as non-specific immune cells and antigen presenting cells in local mucosal sites. Immune cells haven't been observed in grouper eye’s corn. Density of immune cells in the gill sinusoids is significant higher than in peripheral blood. A series of interesting figures showing skin epithelia enveloping a substance has been described and speculated to a process of epithelia engulfing an exogenic substance. Antigen uptake and presentation of mucusal-associated immune tissue has been discussed. Immune cells distributed in mucosal tissues supply cell foundation for mucosal immune system to finish immune response independent of the systematic immune system. The non-specific and specific immune response of mucosal immune system,and whether the mucosal immune system is independent from the systematic immune system have also been discussed in this paper.
    • FullText(HTML)
    • References (21)
  • [1]
    Li G P,Yang T. Reviews on mucosal immune system [J]. J Cell Mol Immunol 2001,17 (6): 594-597[李国平,杨恬.黏膜免疫系统的研究进展. 细胞及分子免疫学杂志,2001,17(6): 594-597]
    [2]
    Vancikova Z. Mucosal immunity-basic principles,ontogeny,cystic fibrosis and mucosal vaccination [J]. Cuur Drug Targets Immune Endocr Metabol Disor,2002,2(1): 83-95
    [3]
    Li A H,Wu Y S,Cai T Z,et al. Development and immune efficacy of a bivalent anti-aeromonas hydrophila and anti-vibrio fluvialis vaccine in carassius auratus linnaeus [J]. Acta Hydrobiologica Sinica,2002,26(1): 52-26 [李爱华,吴玉深,蔡桃珍等.嗜水气单胞菌和河弧菌二联疫苗对鲫的免疫效果.水生生物学报,2002,26(1): 52-26]
    [4]
    Luo X C,Li Z Q,Wang J H,et al. The humoral immune responses of grass carp (Ctenopharyngodon idellus) and the antibody producing cells [J]. J Huazhong Agr U,2000,19(6): 581-584 [罗晓春,李正秋,王金虎,等.草鱼的体液免疫应答及抗体产生细胞.华中农业大学学报,2000,19(6):581-584]
    [5]
    Luo X C,Li Z Q,Wang J H,et al. Seperation and identification of different lymphocyte populations from grass carp (Ctenopharyngodon idellus) [J]. J Huazhong Agr U,2001,20(1): 63-66 [罗晓春,李正秋,王金虎,等.草鱼不同类群淋巴细胞的分离及鉴定.华中农业大学学报,2001,20(1):63-66]
    [6]
    Wu R Q,Li J,Zhang P J,et al. The lymphocytes transplant test and cell-mediated immune of paralichthys olivaceus [J].Acta Hydrobiolo-gica Sinica, 2003,27(2): 127-131 [乌日琴,李军,张培军等. 牙鲆淋巴细胞转化和细胞免疫. 水生生物学报,2003,27(2): 127-131]
    [7]
    Clerton P,Trouaud D,Deschaux P. The chemiluminescence response of leucocytes isolated from the gut of rainbow trout (Oncorhynchus mykiss) [J]. Fish Shellfish Immunol,1998,8:73-76
    [8]
    Press C.M,Evensen O. The morphology of the immune system in teleost fishes [J]. Fish Shellfish Immunol,1999,9:309-318
    [9]
    Lin S H,Dav Idson G A,Secombes,et al. A morphological study of cells isolated from the perfused gill of dab and Atlantic salmon [J].J Fish Biol,1998,53:560-568
    [10]
    Fast M D,Sims D E,Burka,J F, et al. Skin morphology and humoral non-specific defence parameters of mucus and plasma in rainbow trout,coho and Atlantic salmon [J]. Comp Biochem Phys A, 2002,132: 645-657
    [11]
    McMillan D N,Secombes C J. Isolation of rainbow trout (Oncorhynchus mykiss) intestinal intraepithelial lymphocytes (IEL) and measurement of their cytotoxic activity [J]. Fish Shellfish Immunol, 1997,7:527-541
    [12]
    Founier-betz V,Quentel C,Lamour F,et al. Immunocytochemical detection of Ig-positive cells in blood,lymphoid organs and the gut associated lymphoid tissue of the turbot (Scophthalmus maximus) [J]. Fish Shellfish Immunol, 2000,10:187-202
    [13]
    Strand H K,Dalmo R A. Absorption of immunomodulating,(1,3)-glucan in yolk sac larvae of Atlantic halibut,Hippoglossus hippoglossus (L.)[J]. J Fish Dis, 1997,20:41-49
    [14]
    Hellio C,Pons A M,Beaupoil C,et al. Antibacterial,antifungal and cytotoxic activities of extracts from fish epidermis and epidermal mucus [J].Int J Antimicrob Ag, 2002,20:214-219
    [15]
    Ross N W,Firth K J,Wang A P,et al. Changes in hydrolytic enzyme activities of naive Atlantic salmon Salmo salar skin mucus due to infection with the salmon louse Lepeophtheirus salmonis and cortisol implantation [J]. Dis Aquat Organ,2000.41(1): 43-51
    [16]
    Lin S H,Ellis A E,Davidson G A,el al. Migratory,respiratory burst and mitogenic responses of leucocytes isolated from the gills of rainbow trout (Oncorhynchus mykiss) [J]. Fish Shellfish Immunol,1999,9:211-226
    [17]
    Davidson G A,Ellis A E. Secombes C J. Cellular responses of leucocytes isolated from the gut of rainbow trout, Oncorhynchus mykiss (Walbaum) [J]. J Fish Ds,199l,14:651-659
    [18]
    Scapigliati,G,Romano N,Picchietti S,et al. Monoclonal antibodies against sea bass Dicentrarchus labrax (L.) immunoglobulins: immunolocalisation of immunoglobulin-bearing cells and applicability in immunoassays [J]. Fish Shellfish Immunol, 1996,6: 383-401
    [19]
    Rombout J H W M,Taverne-Thiele A J,Villena M I. The gut-associa-ted lymphoid tissue (GALT) of carp (Cyprinus carpio L.) [J]. Dev Comp Immunol,1993,17: 55-66
    [20]
    Moore J.D,Ototake M,Nakanishi T. Particulate antigen uptake during immersion immunisation of fish: The effectiveness of prolonged exposure and the roles of skin and gill [J]. Fish Shellfish Immunol, 1998, 8:393-407
    [21]
    Joosten P. H. M.,Tiemersm A E.,Threels,A.,et al. Oral vaccination of fish against Vibrio anguillarum using alginate microparticles[J]. Fish Shellfish Immunol, 1997,7:471-485
    • Related Articles

Catalog

    Get Citation
    PDF
    XML
    Article views (1132) PDF downloads (625) Cited by()
    Related

    Editorial Office of Acta Hydrobiologica Sinica

    Address：No. 7 Donghu South Road, Wuchang District, Wuhan, Hubei Province, China

    Tel：027-68780701；027-68780800 E-mail：acta@ihb.ac.cn  

    Supported byBeijing Renhe Information Technology Co. Ltd  Technical support：info@rhhz.net

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return