Volume 35 Issue 1
Aug.  2014
Turn off MathJax
Article Contents
Abstract
References
Related Articles
XU Sheng-Wei, LI Tian, WU Jin-Ying, LIANG Jian-Hong, LIU Han-Sheng, LIAN Chang-Ping. CLONING AND EXPRESSION OF WHITE CLOUD MOUNTAIN MINNOW(TANICHTHYS ALBONUBES) CASPASE-3 AND CASPASE-9 cDNAs[J]. ACTA HYDROBIOLOGICA SINICA, 2011, 35(1): 138-144.
Citation: XU Sheng-Wei, LI Tian, WU Jin-Ying, LIANG Jian-Hong, LIU Han-Sheng, LIAN Chang-Ping. CLONING AND EXPRESSION OF WHITE CLOUD MOUNTAIN MINNOW(TANICHTHYS ALBONUBES) CASPASE-3 AND CASPASE-9 cDNAs[J]. ACTA HYDROBIOLOGICA SINICA, 2011, 35(1): 138-144.
shu

CLONING AND EXPRESSION OF WHITE CLOUD MOUNTAIN MINNOW(TANICHTHYS ALBONUBES) CASPASE-3 AND CASPASE-9 cDNAs

  • Institute of Aquatic Economic Animals and Guangdong Provincial Key Laboratory for Aquatic Economic Animals, School of Life Sciences, Sun Yat-Sen(Zhongshan) University, Guangzhou 510275;
    2. Guangzhou Management Station of Ocean and Fisheries Natural Reserves, Guangzhou 510405

  • Received Date: November 23, 2009
  • Published Date: January 24, 2011
    Graphical Abstract
    • Abstract
  • The white cloud mountain minnow(Tanichthys albonubes) is an important aquarium fish,and also a potential experimental model organism.In order to get the knowledge of apoptosis under environmental stress in this species,we have firstly cloned and sequenced the full-length cDNAs of Caspase-3 and Caspase-9 from the gill tissue of T.albo-nubes using homology cloning approach combined with RACE method.In this study,the bioinformatics analysis of Caspase-3 and Capase-9 full-length cDNA sequences were presented.The results indicated that the full-length cDNA of Caspase-3 was composed of 2153 base pairs(bp),which included a 840 bp open reading frame(ORF) encoding a 279-amino acid peptide,whereas the full-length cDNA of Caspase-9 was composed of 1758 bp,which included a 1323 bp ORF encoding a 440-amino acid peptide.The identity comparison and the phylogenetic analysis showed that the predicted Caspase-3 and Caspase-9 proteins of T.albonubes were structurally similar to those of other fish,with high conservation in important functional domains.Real-time PCR analysis showed that the expression levels of Caspase-3 and Caspase-9,under cypermethrin-induced stress conditions,were suppressed compared to control group,and that Caspase-3 and Caspase-9 mRNA levels were significantly affected in a dose-and time-dependent manner by cyperme-thrin.These results suggest that cypermethrin plays a role in the suppression of apoptosis of T.albonubes,and that the anti-apoptotic effect of cypermethrin may be beneficial to this fish so that it can survive in harsh environments.
    • FullText(HTML)
    • References (25)
  • [1]
    Shi F, Lin X T, Sun J, et al. Age structure and growth characteristicsof wild Tanichthys albonubes population [J]. ChineseJournal of Ecology, 2008, 27(12): 2159-2166 [史方,林小涛, 孙军, 等. 自然种群唐鱼的耳石、日龄与生长. 生态学杂志, 2008, 27(12): 2159-2166]
    [2]
    Wang R L, Ma G Z, Fang Z Q. Safety assessment and acutetoxicity of copper, cadmium and zinc to white clound mountainminnow Tanichthys albonubes [J]. Fisheries Science,2006, 25(3): 117-120 [王瑞龙, 马广智, 方展强. 铜、镉、锌对唐鱼的急性毒性及安全浓度评价. 水产科学, 2006,25(3): 117-120]
    [3]
    Wang H Y, Ye X, Bai J J, et al. Isolation of white cloudmountain minnow β-actin promoter and assay of its transcriptionactivity [J]. Journal of Fishery Sciences of China,2008, 15(1): 47-54 [王海英, 叶星, 白俊杰, 等. 唐鱼β-肌动蛋白基因启动子的分离及其驱动活性的检测. 中国水产科学, 2008, 15(1): 47-54]
    [4]
    Wang R L, Chen Y M, Xu J, et al, Effects of cypermethrin onsuperoxide dismutase (SOD) activities in liver and gill tissuesof Tanichthys albonubes [J]. Ecology and Environment,2007, 16(3): 790-793 [王瑞龙, 陈玉明, 徐军, 等. 氯氰菊酯对唐鱼肝和鳃组织超氧化物歧化酶 (SOD) 活性的影响. 生态环境, 2007, 16(3): 790-793]
    [5]
    Mu W, Xia W, Xiong L, et al. Study of metabolizable damagein Carassius auratus caused by Cypermethrin [J]. Journalof Huazhong Normal University, 2008, 42(4): 602-605[牟文, 夏伟, 熊丽, 等. 氯氰菊酯对鲫鱼机体代谢损伤效应研究. 华中师范大学学报, 2008, 42(4): 602-605]
    [6]
    Xing M L, Chen J P, Wang X F, et al. Effect of okadaic acidon apoptosis in human amnion cells [J]. Acta HydrobiologicaSinica, 2007, 31(4): 607-609 [邢鸣鸾, 陈加平, 王晓峰,等. 大田软海绵酸对人胚胎羊膜细胞FL 凋亡的影响. 水生生物学报, 2007, 31(4): 607-609]
    [7]
    Zhang J, Huang B, Gao Q, et al. Molecular cloning andcharacterization of fas-associated death domain (FADD)from Branchiostoma belcheri [J]. Acta HydrobiologicaSinica, 2009, 33(6): 1175-1184 [张晶, 黄贝, 高谦, 等.白氏文昌鱼FADD 的克隆及功能研究. 水生生物学报,2009, 33(6): 1175-1184]
    [8]
    Hengartner M O. The biochemistry of apoptosis [J]. Nature,2000, 407: 770-776
    [9]
    Duan H, Orth K, Chinnaiyan A M, et al. ICE-LAP6, a novelmember of the ICE/Ced-3 gene family, is activated by thecytotoxic T cell protease granzyme B [J]. The Journal ofBiological Chemistry, 1996, 271: 16720-16724
    [10]
    Luo X, Budihardjo I, Zou H, et al. Bid, a Bcl2 interactingprotein, mediates cytochrome C release from mitochondria inresponse to activation of cell surface death receptors [J]. Cell,1998, 94: 481-490
    [11]
    Fernandes-Alnemri T, Litwack G, Alnemri E S. CPP32, anovel human apoptotic protein with homology to Caenorhabditiselegans cell death protein Ced-3 and mammalian interleukin-1 beta-converting enzyme [J]. The Journal of BiologicalChemistry, 1994, 269: 30761-30764
    [12]
    Johnson A L, Bridgham J T. Caspase-3 and -6 expression andenzyme activity in hen granulosa cells [J]. Biology of Reproduction,2000, 62: 589-598
    [13]
    Wang H, Keiser J A. Molecular characterization of rabbitCPP32 and its function in vascular smooth muscle cellapoptosis [J]. American Journal of Physiological Heart andCirculatory Physiology, 1998, 274: H1132-H1140
    [14]
    Yabu T, Kishi S, Okazaki T, et al. Characterization of zebrafishcaspase-3 and induction of apoptosis through ceramidegeneration in fish fathead minnow tailbud cells and zebrafishembryo [J]. Biochemical Journal, 2001, 360: 39-47
    [15]
    Nicholson D W, Ali A, Thornberry N A, et al. Identificationand inhibition of the ICE/CED-3 protease necessary formammalian apoptosis [J]. Nature, 1995, 376: 37-43
    [16]
    Wang X, Zelenski N G, Yang J, et al. Cleavage of sterolregulatory element binding proteins (SREBPs) by CPP32during apoptosis [J]. EMBO Journal, 1996, 15: 1012-1020
    [17]
    Park I S, Moon H R, Seok H, et al. Rearrangement of tryptophanresidues in caspase-3 active site upon activation [J].Biochimica et Biophysica Acta, 2004, 1700: 5-9
    [18]
    Ruoslahti E. RGD and other recognition sequences for integrins[J]. Annual Review of Cell and Developmental Biology,1996, 12: 697-715
    [19]
    Shimizu S, Narita M, Tsujimoto Y. Bcl-2 family proteinsregulate the release of apoptogenic cytochrome C by themitochondrial channel VDAC [J]. Nature, 1999, 399: 483-487
    [20]
    Liu X, Kim C N, Yang J, et al. Induction of apoptotic programin cell-free extracts: requirement for dATP and cytochromeC [J]. Cell, 1996, 86: 147-157
    [21]
    Jiang X, Wang X. Cytochrome C promotes caspase-9 activationby inducing nucleotide binding to Apaf-1 [J]. Journal ofBiological Chemistry, 2000, 275: 31199-31203
    [22]
    Saleh A, Srinivasula S M, Acharya S, et al. Cytochrome Cand dATP-mediated oligomerization of Apaf-1 is a prerequisitefor procaspase-9 activation [J]. Journal of BiologicalChemistry, 1999, 274: 17941-17945
    [23]
    Srinivasula S M, Fernandes-Alnemri T, Zangrilli J, et al. TheCed-3/interleukin 1beta converting enzyme-like homologMch6 and the lamin-cleaving enzyme Mch2alpha are substratesfor the apoptotic mediator CPP32 [J]. Journal of BiologicalChemistry, 1996, 271: 27099-27106
    [24]
    Hu Y, Benedict M A, Wu D, et al. Bcl-XL interacts withApaf-1 and inhibits Apaf-1-dependent caspase-9 activation[J]. Proceedings of the National Academy of Sciences of theUnited States of America, 1998, 95: 4386-4391
    [25]
    Wang Y, Xiong L, Liu X P, et al. Subacute Toxicity of Cypermethrinto Carp [J]. Journal of Agro-Environment Science,2006, 25(1): 200-203 [王媛, 熊丽, 刘喜平, 等. 氯氰菊酯对鲤鱼亚急性毒性研究. 农业环境科学学报, 2006,25(1): 200-203][26] Xie W P, Zhu X P, Chen K C, et al. Toxic effects of cypermethrinon Na+/K+-ATP activity and ultramicrostructure ofgill and liver tissue in grass carp [J]. Journal of Fishery Sciencesof China, 2009, 16(1): 120-126 [谢文平, 朱新平,陈昆慈, 等. 氯氰菊酯对草鱼组织Na+/K+-ATP 酶活性及肝、鳃超显微结构的影响. 中国水产科学, 2009, 16(1):120-126]
    • Related Articles

  • Related Articles

    [1]YAN Han-Xiao, SHI Jian-Wu, SHENG Jun-Qing, WANG Jun-Hua, Hong Yi-Jiang. THE STRUCTURAL FEATURE AND EXPRESSION ANALISIS OF HSβ-CATENIN cDNA AND PROTEIN FROM HYRIOPSIS SCHLEGELII[J]. ACTA HYDROBIOLOGICA SINICA, 2017, 41(5): 972-976. DOI: 10.7541/2017.121
    [2]MA Jin-Wu, LÜ Jian-Jian, LIU Ping, GAO Bao-Quan, LI Jian. NA+/H+-EXCHANGER IN SWIMMING CRAB (PORTUNUS TRITUBERCULATUS): CLONING, CHARACTERIZATION AND MRNA EXPRESSION UNDER SALINITY STRESS[J]. ACTA HYDROBIOLOGICA SINICA, 2016, 40(5): 902-907. DOI: 10.7541/2016.116
    [3]SUN Lu-Ming, YU Meng-Jun, CHEN Ya-Dong, CHEN Xue-Jie, LIU Yang, QIU Xue-Mei, SHA Zhen-Xia. AKT-INTERACTING PROTEIN GENE CLONING AND ITS EXPRESSION PROFILE IN RESPONSE TO PATHOGEN INFECTION IN HALF SMOOTH TONGUE SOLE (CYNOGLOSSUS SEMILAEVIS)[J]. ACTA HYDROBIOLOGICA SINICA, 2016, 40(3): 467-473. DOI: 10.7541/2016.62
    [4]XU Sheng-Wei, LI Tian, WU Jin-Ying, LIANG Jian-Hong, LIU Han-Sheng, LIAN Chang-Ping. CLONING AND EXPRESSION OF WHITE CLOUD MOUNTAIN MINNOW(TANICHTHYS ALBONUBES) CASPASE-3 AND CASPASE-9 cDNAs[J]. ACTA HYDROBIOLOGICA SINICA, 2011, 35(1): 138-144.
    [5]SU Jian-Ming, ZHANG Huai-Yun, CHEN Tao, XIANG Jian-Guo, XIAO Tiao-Yi. CLONING, SEQUENCINGα1-MICROGLOBULIN/BULININPRECURSO cDNA OFGRASSCARP, CTENOPHARYNGODONIDELLUS[J]. ACTA HYDROBIOLOGICA SINICA, 2009, 33(2): 183-188.
    [6]QU Xian-Cheng, LIU Ying, YANG Yan-Hong, CUI Yan-Hui, ZHOU Zheng-Feng. MOLECULAR CLONING AND SEQUENCE ANALYSIS OF THE TWO GONADOTROPIN B-SUBUNIT cDNAs OF BLUNT SNOUT BREAM(MEGALOBRAMA AMBLYCEPHALA)[J]. ACTA HYDROBIOLOGICA SINICA, 2007, 31(3): 377-385.
    [7]FANG Ping, LI Ying-Wen, HU Wei, DING Shi-Hua. CLONING AND SEQUENCE ANALYSIS OF cDNA ENCODING cGnRH-II FROM RICE FIELD EEL(MONOPTERUS ALBUS)[J]. ACTA HYDROBIOLOGICA SINICA, 2007, 31(2): 259-264.
    [8]ZHANG Xue-Jun, QU Gang, ZHU Wen-Li, ZHANG Li, WANG Juan, WU Jiang, LIU Han-Yuan, CHEN Fang, XU Heng. CONSTRUCTION OF INTESTINAL cDNA LIBRARY AND ANALYSIS OF SOME EXPRESSED SEQUENCE TAGS SEQUENCING OF CTENOPHARYNGODON IDELLUS[J]. ACTA HYDROBIOLOGICA SINICA, 2007, 31(2): 251-258.
    [9]LI Jun-Sheng, LI Jian-Lin, WU Ting-Ting. CLONING AND SEQUENCING OF TRYPSINⅠMRNA IN TILAPIA[J]. ACTA HYDROBIOLOGICA SINICA, 2006, 30(2): 209-213.
    [10]Chen Yueqin, He Jiawan, Zhuang Li, Zeng Longmei, Qu Lianghu. SEQUENCE ANALYSIS OF rDNA 16S-23S INTERGENIC SPACER FROM MICROCYSTIS AERUGINOSA AND MICROCYSTIS WESENBERGII IN DONGHU LAKE, CHINA[J]. ACTA HYDROBIOLOGICA SINICA, 1999, 23(1): 41-46.

Catalog

    Get Citation
    PDF
    XML
    Article views (1040) PDF downloads (667) Cited by()
    Related

    Editorial Office of Acta Hydrobiologica Sinica

    Address：No. 7 Donghu South Road, Wuchang District, Wuhan, Hubei Province, China

    Tel：027-68780701；027-68780800 E-mail：acta@ihb.ac.cn  

    Supported byBeijing Renhe Information Technology Co. Ltd  Technical support：info@rhhz.net

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return