WEN Jian-fan. NUCLEAR MATRIX AND NUCLEAR LAMINA OF EUGLENA GRACILIS[J]. ACTA HYDROBIOLOGICA SINICA, 2001, 25(6): 551-557.
Citation: WEN Jian-fan. NUCLEAR MATRIX AND NUCLEAR LAMINA OF EUGLENA GRACILIS[J]. ACTA HYDROBIOLOGICA SINICA, 2001, 25(6): 551-557.

NUCLEAR MATRIX AND NUCLEAR LAMINA OF EUGLENA GRACILIS

  • Received Date: June 10, 1999
  • Rev Recd Date: April 19, 2001
  • Published Date: November 24, 2001
  • Lightly sonicated E. gracilis cells were extracted sequentially, and then their nuclear matrix and nuclear lamina were investigated by DGD embedment-free electron microscopy and Western blot analysis. The results showed that in the nucleus there were a fibrous network and a layer of lamina beneath the nuclear membrane, both of which could not be removed by DNase and hot trichloroacetic acid. Western blot analysis(with polyclonal antiserum against three lamins of rat liver)showed that its nuclear lamina possessed a major and a minor positive polypeptides, which were similar to mammalian lamin B and lamin A respectively. It was suggested that the lower unicellular eukaryote E. gracilis already had nuclear matrix and nuclear lamina, its lamina polypeptide(lamin)should reflect a historical stage of nuclear lamina evolution.
  • [1]
    Verheijen R. van Venrooij W,Ramaekers F. The nuclear matrix:structure and composition [J]. J Cell Sci, 1988,90:11-36[2] 文建凡.核骨架-细胞核内的重要结构体系[J]. 动物学研究,1998,19(4):382-385[3] Cavalier-Smith T. Membrane heredity,symbiogenesis,and the multiple origins of algae. In Biodiversity and evolution(ed. R. Arai,M. Kato,and Y.Doi),The national science museum foundation,Tokyo. 1995. 69-107[4] 文建凡,李靖炎.最原始的真核生物-源真核生物(Archezoa)的核骨架研究[J]. 中国科学(C辑),1998,28(5):423-430[5] 文建凡,吴传芬,李靖炎.特殊涡鞭毛虫-尖尾藻的核骨架[J]. 动物学研究,1992,13(1):89-94[6] Fey E G,Krochmalnic G,Penman S. The nonchromatin substructures of the nucleus:the ribonucleoprotein(RNP)-containing ans RNP-depleted matrices analyzed by sequential fractionation ans resinless section electron microscopy. [J]. J Cell Biol, 1986,102:1654-1665[7] Laemmli U K. Cleavage of structural protein during the assembly of the head of bacteriophage T 4[J]. Nature,1975,226:680-685[8] Towbin H,Staehelin T,Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets:procedure and some application [J] Proc Natl Sci, USA,1979,76:4350-4354[9] Berezney R. Organization and functions of the nuclear matrix. in:Chromososmal Nonhistone Proteins-Structural Associations,Vol. lV. Hnilica,L.S.CRC Press,lnc. Boca Raton. 1984,4:119-180[10] Razin S V,Gromova ll. The channels model of nuclear matrix structure[J]. BioEssays,1995,17:443-450[11] 文建凡.从低等单细胞真核生物的核骨架看核骨架的起源进化[J]. 大自然探索,1996,15(2):59-64[12] 蔡树涛,翟中和,曾丛梅,等.寇氏隐甲藻细胞核骨架与中间纤维的研究[J]. 实验生物报,1991,24:33-38[13] 方志林,吴传芬,李靖炎.前环藻细胞核骨架及其真实性的研究[J]. 动物学研究,1993,14(1):80-85[14] 文建凡. 涡鞭毛虫类的染色质碱性蛋白研究及其进化地位的重新认识[J]. 科学通报,1998,43(16):1523-1535[15] Lehner C F,Stick R,Eppenberger H M,et al. Differential expression of nuclear lamin proteins during c1995. icken development[J]. J Cell Biol,1987,105:577-587[16] Steward C,Burke B. Teratocarcinoma stem cells and early mouse embryos contain only a single major lamin polypeptide closely resembling lamin B[J]. Cell,1987,51:383-392

    Verheijen R. van Venrooij W,Ramaekers F. The nuclear matrix:structure and composition [J]. J Cell Sci, 1988,90:11-36[2] 文建凡.核骨架-细胞核内的重要结构体系[J]. 动物学研究,1998,19(4):382-385[3] Cavalier-Smith T. Membrane heredity,symbiogenesis,and the multiple origins of algae. In Biodiversity and evolution(ed. R. Arai,M. Kato,and Y.Doi),The national science museum foundation,Tokyo. 1995. 69-107[4] 文建凡,李靖炎.最原始的真核生物-源真核生物(Archezoa)的核骨架研究[J]. 中国科学(C辑),1998,28(5):423-430[5] 文建凡,吴传芬,李靖炎.特殊涡鞭毛虫-尖尾藻的核骨架[J]. 动物学研究,1992,13(1):89-94[6] Fey E G,Krochmalnic G,Penman S. The nonchromatin substructures of the nucleus:the ribonucleoprotein(RNP)-containing ans RNP-depleted matrices analyzed by sequential fractionation ans resinless section electron microscopy. [J]. J Cell Biol, 1986,102:1654-1665[7] Laemmli U K. Cleavage of structural protein during the assembly of the head of bacteriophage T 4[J]. Nature,1975,226:680-685[8] Towbin H,Staehelin T,Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets:procedure and some application [J] Proc Natl Sci, USA,1979,76:4350-4354[9] Berezney R. Organization and functions of the nuclear matrix. in:Chromososmal Nonhistone Proteins-Structural Associations,Vol. lV. Hnilica,L.S.CRC Press,lnc. Boca Raton. 1984,4:119-180[10] Razin S V,Gromova ll. The channels model of nuclear matrix structure[J]. BioEssays,1995,17:443-450[11] 文建凡.从低等单细胞真核生物的核骨架看核骨架的起源进化[J]. 大自然探索,1996,15(2):59-64[12] 蔡树涛,翟中和,曾丛梅,等.寇氏隐甲藻细胞核骨架与中间纤维的研究[J]. 实验生物报,1991,24:33-38[13] 方志林,吴传芬,李靖炎.前环藻细胞核骨架及其真实性的研究[J]. 动物学研究,1993,14(1):80-85[14] 文建凡. 涡鞭毛虫类的染色质碱性蛋白研究及其进化地位的重新认识[J]. 科学通报,1998,43(16):1523-1535[15] Lehner C F,Stick R,Eppenberger H M,et al. Differential expression of nuclear lamin proteins during c1995. icken development[J]. J Cell Biol,1987,105:577-587[16] Steward C,Burke B. Teratocarcinoma stem cells and early mouse embryos contain only a single major lamin polypeptide closely resembling lamin B[J]. Cell,1987,51:383-392
  • Related Articles

    [1]HU Shao-Qiu, DUAN Rui, ZHANG Dong-Xu, BAO Jiang-Hui, LÜ Hua-Fei, DUAN Ming. CLASSIFICATION OF 3D POINT CLOUD MODELS OF FISH BASED ON POINT TRANSFORMER APPROACH[J]. ACTA HYDROBIOLOGICA SINICA, 2025, 49(2): 022515. DOI: 10.7541/2024.2024.0053
    [2]LI Zhong, LIANG Hong-Wei, ZOU Gui-Wei. A RAPID PCR-QUALITY DNA EXTRACTION METHOD IN FISH[J]. ACTA HYDROBIOLOGICA SINICA, 2012, 36(2): 365-367. DOI: 10.3724/SP.J.1035.2012.00365
    [3]Shahid Mahboob, Bilal Hussain, Zahid Iqbal, Abdul Shakoor Chaudhry. ESTIMATION OF VOLATILE CONSTITUENTS IN THE FISH FLESH FROM WILD AND FARMED CIRRHINA MRIGALA AND CYPRINUS CARPIO[J]. ACTA HYDROBIOLOGICA SINICA, 2009, 33(3): 484-491.
    [4]YANG Wei-Dong, ZHANG Xin-Lian, LIU Jie-Sheng, GAO Jie, ZHANG Ping. INHIBITORY EFFECT AND SINKING BEHAVIOUR OF WOOD MEALS FROM CHINA FIR ON ALEXANDRIUM TAMARENSE IN CULTURES[J]. ACTA HYDROBIOLOGICA SINICA, 2005, 29(2): 215-219.
    [5]HU Yong, KONG Dong-Dong, HE Yi-Kun. ON-LINE CLONING AND EVOLUTION ANALYSIS OFCrMinD GENE FROMCHLAMYDOMONA SREINHARDTII[J]. ACTA HYDROBIOLOGICA SINICA, 2004, 28(1): 106-109.
    [6]XIAO Wu-han, ZHANG Ya-ping. GENETICS AND EVOLUTION OF MITOCHONDRIAL DNA IN FISH[J]. ACTA HYDROBIOLOGICA SINICA, 2000, 24(4): 384-391.
    [7]Guo Youhao, Huang Shuangquan, Chen Jiakuan. BREEDING SYSTEM AND EVOLUTION OF AQUATIC ANGIOSPERMS[J]. ACTA HYDROBIOLOGICA SINICA, 1998, 22(1): 79-85.
    [8]Wu Jihua, Liang Yanling, Sun Xida. NEWLY RECORDED SPECIES OF FREE-LIVING NEMATODES FROM CHINA (CHROMADORIDA,ENOPLIDA ARAEOLAIMIDA)[J]. ACTA HYDROBIOLOGICA SINICA, 1997, 21(4): 320-321.
    [9]Zhou Dinggang, Wang kangning. EFFECTS OF RESERPINE AND DOMPERIDONE ON LHRH-A INDUCED OVULATION IN MONOPTERUS ALBUS[J]. ACTA HYDROBIOLOGICA SINICA, 1993, 17(1): 98-100.
    [10]Xiao Xiao, Wang Esheng, Shen Zonghou. A COMPARATIVE STUDY THE ELECTROPHORRETIC PATTERNS OF SMALL NUCLEAR RNAs FROM LIVER OF FOUR FISH SPECIES[J]. ACTA HYDROBIOLOGICA SINICA, 1988, 12(1): 78-81.

Catalog

    Article views (919) PDF downloads (403) Cited by()
    Related

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return