SCREENING OF DIFFERENTIALLY EXPRESSED GENES AT GASTRULA STAGE DURING EMBRYOGENESIS OF CARASSIUS AURATUS GIBELIO

LIU Jing-Xia; SHI Yao-Hua GUI; Jian-Fang

Volume 29 Issue 4

Aug. 2014

Turn off MathJax

Article Contents

Abstract

References

ACTA HYDROBIOLOGICA SINICA > 2005 > 29(4): 359-365.

LIU Jing-Xia, SHI Yao-Hua GUI, Jian-Fang. SCREENING OF DIFFERENTIALLY EXPRESSED GENES AT GASTRULA STAGE DURING EMBRYOGENESIS OF CARASSIUS AURATUS GIBELIO[J]. ACTA HYDROBIOLOGICA SINICA, 2005, 29(4): 359-365.

Citation:

PDF (428 KB)

SCREENING OF DIFFERENTIALLY EXPRESSED GENES AT GASTRULA STAGE DURING EMBRYOGENESIS OF CARASSIUS AURATUS GIBELIO

Institute of Hydrobiology, The Chinese Academy of Sciences, State Key Laboratory of Freshwater Ecology and Biotechnology;
Graduate School of the Chinese Academy of Sciences, Wuhan 430072

Received Date: September 08, 2004
Rev Recd Date: October 24, 2004
Published Date: July 24, 2005

Graphical Abstract

Abstract

Abstract

Embryogenesis is a progressive and complicated process that is regulated by differential gene activity. In order to identify some differentially expressed genes in early embryogenesis,two kinds of SMART cDNAs were respectively synthesized frommature eggs and gastrula embryos of Carassius auratus gibelio,and the gastrula embryo SMART cDNA library was constructed.Following this program,131 positive clones were screenedfrom about 1500 clones of the gastrula embryo SMARTcDNA library bytwo rounds of dotblot differential hybridizations using the synthesized SMARTcDNAs as probes. Furthermore,58 positive cloneswere selected for sequencing. Searching GenBank by the nucleotide sequences indicated that most of the sequenced cDNAs encoded ribosome proteins and regulators involved in translation and transcription,and some encoded lipoproteins involved in themetabolism of fat. And,some unknown new genes were also identified from the screening. Moreover,we investigated the expression patternsof 6 genes during embryogenesis by RTPCR analysis,and confirmed their differential expression during embryogenesis. Four different expression patterns,such as transcribing again at gastrula based on the maternal products,beginning to transcribe at blastula stage, beginning to transcribe at gastrula stage,and transcribing only during embryogenesis, were revealed inthe analyzed genes. Through this study,we cloned and identified some genes expressed differentially at early stage during embryo2genesis,which will be a solid base for further researches on their functions and regulative mechanisms during embryogenesis.
- Carassius auratus gibelio,
- Gastrula,
- Embryogenesis,
- SMART cDNA library,
- Differential screening

FullText(HTML)

References (31)

References

[1]	Gui J F, Y i M S. Developmental Biology [M]. Beijing: Sciencepress. 2002,113[桂建芳,易梅生,发育生物学,北京:科学出版社.2001,113]
[2]	Gui J F. Zebrafish, the ideal model for the studyof molecular devel2opmental biology[J]. Bioengineering progress. 1995,16(3):30-33[桂建芳。分子发育学研究的理想模式2斑马鱼。生物工程进展.1995,16(3):30-33]
[3]	Zhu Z Y, Sun YH. Embryonic and genetic manipulation infish[J].Cell Reseach. 2000,10(1):17-27
[4]	Ober E A,Field H A, Stainier D Y R. From endoderm formation toliver and pancreas development in zebrafish[J]. Mech. of Dev.2003,120:5-18
[5]	Rebagliati M R, T oyama R, Fricke C, et al. Zebrafish Nodal Related Genes Are Implicated in Axial Patterning and Establishing Left2Right Asymmetry[J]. Developmental Biology,1998,199:261-272
[6]	K ikuchi Y,Agathon A, Alexander J, et al.Casanova encodes anovel Sox related protein necessary and sufficient for early endodermformation in zebrafish[J]. Genes &Development,2001,15:1493-1505
[7]	Feldman B, Gates M A,Egan E S, et al. Zebrafish organizer development and germ2layer formation require nodal2related signals[J].Nature, 1998,395:181-185
[8]	Rebagliati M R, T oyama R, Haffter P, et al.cyclops encodes anodal2related factor involved in midline signaling[J].Proc. Natl.Acad. Sci.1998b,95:9932-9937
[9]	ZhangJ, Talbot W S, Schier A F. Positional cloning identifies ze2brafish one2eyed pinhead as a permissive EGF related ligand requiredduring gastrulation[J]. Cell, 1998,92:241-251
[10]	K ikuchi Y,Trinh L A, Reiter J F, et al. The zebrafish bonnie andclyde gene encodes a Mix family homeodomain protein that regulatesthe generation of endodermal precursors[J].Genes & Development,2000,14:1279-1289
[11]	Henry GL,Melton D A. Mixer, a homeobox gene required for endoderm development[J]. Science. 1998,281:91-96
[12]	Jiang Y G, Liang S C, Cheng B D. The biological effect of heteroge2nous sperms in the gynogenesis offspringof silver crucian carp(Caras2sius auratus gibelio-[J]. Acta Hydrobiologica Sinica.1983,8(1):1-13[蒋一 ,梁绍昌,陈本德。异源精子在银鲫雌核发育子代中的生物学效应。水生生物学报。1983,8(1):1-13]
[13]	Gui J F, Liang S C,ZhuL F, et al. Discoveryof two different reproductive development modes of the eggs of artificial multiple tetraploidallogynogenetic silver crucian carp [J].Chin.Sci.Bull. 1993,38(4),332-337
[14]	Gui J F. A unique study system: gynogenetic fish, Carassius auratusgibelio[J]. Sci. Found. China. 1996,4:44-46
[15]	Gui J F. Retrospect and prospect of studieson the mechanismof natural gynogenesis in silver crucian carp (Carassius auratus gibelio)[J]. Bull. Nat. Natl. Sci. Found. China.1997,11:11-16
[16]	Zhou L,Wang Y,Gui J F. Genetic evidence for gonochoristic reproduction in gynogenetic silver crucian carp (Carassius auratus gibelioBloch-as revealed by RAPD assays[J]. J. Mol.Evol. 2000,51:498-506
[17]	Zhou L,Gui J F. Karyotypic diversity in polyploid gibel carp, Carassius auratus gibelioBloch[J]. Genetica.2002,115(2):223-232
[18]	Shi YH, LiuJ, Xia J H, Gui J F. Sreenfor stage specific expressiongenes between tail bud stage and heartbeat beginning stage in embryogenesis of gynogenetic silver crucian carp [J]. Cell Research.2002,12(2):133-142
[19]	Liu J, Shi Y H, Y ing J, et al. Sreen for stage specific expressiongenes between gastrula and tail bud stage in embryogenesis of gynogenetic silver crucian carp[J]. Acta Genetica Sinica.,2004,in press[刘军,石耀华,尹隽,等,雌核发育银鲫原肠期胚胎和尾芽期胚胎差异表达基因的呈现。遗传学报,2005,印刷中]
[20]	Liu J, Shi Y H, Y ing J, et al. The cloning and characterization oftwo gene related to protein synthesize in gebil carp[J]. Acta Hydro2biologica Sinica., 2003,27(5):512-520[刘军,石耀华,尹隽,等。银鲫两个蛋白合成相关基因全长 cDNA 的克隆及其特征分析。水生生物学报,2003,27(5):512-520]
[21]	Liu J, Shi Y H, Y ingJ, et al. Cloning and characterization of fourfull length cDNA of hatching enzyme genes in gynogenetic gibel carp[J]. High Technology Letters,2003,151(13):38-45[刘军,石耀华,尹隽,等。雌核发育银鲫四种孵化酶基因全长 cDNA 的克隆及其特征分析[J]。高技术通讯。2003,151(13):38-45]
[22]	Shi YH, LiuJ, Xia J H, et al. Cloning and expressional characteri2zation of creatine kinase M32CKin the gibel carp (Carassius auratusgibelio-[J]. Acta Zoologica Sinica. 2003,49(5):637-645[石耀华,刘军,夏建红,等,银鲫肌酸激酶 M32CKcDNA 的克隆及其表达特征。动物学报,2003,49(5):637-645]
[23]	Kajiwara K, Nagawawa H, Shimizu2Nishikawa K, et al. Molecularcharacterization of seizue2related genes isolated by differential screening [J]. Biochem.Biophys.Res.Communications. 1996,219:795-799
[24]	Wu C, Gui J F, Fish Genetics and Breeding Engineering [M].Shanghai:Scientific and Technical Publishers,. 1999,41-62[吴清江,桂建芳。鱼类遗传育种工程。上海:科学技术出版社。1999,41-62]
[25]	Zhang YB, Shi Y H, Gui J F, Construction of antiviral subtractivecDNA library of cultured fish cells[J]. Acta Hydrobiologica Sinica2003;27(1):113-118[张义兵,石耀华,桂建芳。鱼类培养细胞抗病毒差减 cDNA 文库的构建。水生生物学报,2003,27(1):113-118]
[26]	Diatchenko L, Lau Y F C, Campbell A P, et al. Supperssion subtractive hybridization:a method for generating differentially regulatedor tissue specific cDNA probes and libraries [J].Proc.Natl.Acad. Sci. USA.1996,93:6025-6030
[27]	Xie J, Wen J J, Chen B. Differential gene expression in fully2grownoocytes between gynogenetic and gonochoristic crucian carps [J].Gene.2001,271:109-116
[28]	Zhang YB, Zhang Q Y, Xu D Q, et al. Identification of antiviral relevant genes in the cultured fish cells induced by inactivated virus[J]. Chinese Science Bulletin, 2003. 48(6):581-588
[29]	Lammert E, Cleavera O, Meltona D. Role of endothelial cells in ear2ly pancreas and liver development[J]. Mechanisms of Development,2003,120:59-64
[30]	Field H A, Ober E A, Roeser T, et al. Formation of the digestivesystem in zebrafish:I.Liver morphogenesis. Dev. Biol., 2002,253(2):279-290
[31]	Wu L H. Gene engineering principle[M]. Beijing:Scientific press,1998[吴乃虎。基因工程原理(上-。北京:科学出版社,1998]

Cited By

Get Citation

PDF

XML

Article views (946) PDF downloads (606)

SCREENING OF DIFFERENTIALLY EXPRESSED GENES AT GASTRULA STAGE DURING EMBRYOGENESIS OF CARASSIUS AURATUS GIBELIO

Abstract

References

Catalog

Related

Export File

Citation

Format

Content