Volume 25 Issue 3
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ZHANG Qing-hua, CHEN Ying, LU Cheng-ping. SURVIVAL AND STABILITY OF GREEN FLUORESCENT PROTEIN MARKED(GFP-MARKED)AEROMONAS HYDROPHILA IN WARM WATER[J]. ACTA HYDROBIOLOGICA SINICA, 2001, 25(3): 251-256.
Citation: ZHANG Qing-hua, CHEN Ying, LU Cheng-ping. SURVIVAL AND STABILITY OF GREEN FLUORESCENT PROTEIN MARKED(GFP-MARKED)AEROMONAS HYDROPHILA IN WARM WATER[J]. ACTA HYDROBIOLOGICA SINICA, 2001, 25(3): 251-256.
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SURVIVAL AND STABILITY OF GREEN FLUORESCENT PROTEIN MARKED(GFP-MARKED)AEROMONAS HYDROPHILA IN WARM WATER

  • Key Lab of Animal Disease Diagnostic and Immunology, Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095

  • Received Date: February 11, 1999
  • Rev Recd Date: September 29, 2000
  • Published Date: May 24, 2001
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  • The survival and stability of Aeromonas hydrophila(Ah)strain 4332GFP marked with green fluorescent protein plasmid(pGFP)in the warm water was tested. The results showed that Ah4332GFP survived 47 d and the number of green fluorescent cells(GFCs)had two peaks in the autoclaved pond water. In the natural pond water, Ah4332GFP survived 18 d, but the number of GFCs declined during the experimental period. It suggested that Ah4332GFP and the natural bacteria in the pond water interacted and affected the stability of marked bacteria. In the medium M9, Ah4332GFP strain could culture to 40 and 70 generations and the rates of stability of pGFP were 47.3% and 20.5%, respectively. It concluded that pGFP had relative stability and Ah4332GFP could be applied to bacterial ecology survey.
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  • [1]
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    陈怀青,陆承平.家养鲤科鱼暴发传染病的病原研究[J].南京农业大学学报,1991,14(4);87—91[2] Chalfie M,Tu Y,Euskirchen G,et al.Green fluorescent protein as a marker for gene expression [J].Science,1994,263(11):802—805[3] Sambrook J,Fritsch E F,Maniatis T et al.Molecular Cloning: A Laboratory Manual,(second edition)[M].New York: Cold Spring Harbor Laboratory,1989.[4] Leff L G,Leff A A.Use of green fluorescent protein to monitor survival of genetically engineered bacteria in aquatic environments [J].Appl.Environ.Microbiol,1996,9:3486—3488[5] 王扬,张志毅,杨胜利,等.Par区域的克隆及其对质粒pUC9稳定性的影响[J].生物工程学报,1992,8(2);134—139[6] 凌红丽,陆承平,陈怀青,等.嗜水气单胞菌检验程序的研究[J].中国动物检疫,1998,15(3);1—3[7] Kurland C G,Dong H.Bacterial growth inhibition by overproduction of protein [J].Molecular Microbiology,1996,21(1):1—4[8] 毛牧灵,Lassana T B,沈萍,等.含par位点的重组质粒pSJM3的构起家主其稳定性研究[J].生物工程学报,1998,14(1);64—69
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