Volume 34 Issue 2
Aug.  2014
Turn off MathJax
Article Contents
Abstract
References
CHEN Ming-Xiu, ZHANG Li-Qiang, WEN Chun-Gen, SUN Jun, GAO Qian. PHYLOGENETIC RELATIONSHIP OF SPECIES IN THE GENUS ASPIDOGASTER (ASPIDOGASTRIDAE, ASPIDOGASTRINAE) IN CHINA AS INFERRED FROM ITS rDNA SEQUENCES[J]. ACTA HYDROBIOLOGICA SINICA, 2010, 34(2): 313-316.
Citation: CHEN Ming-Xiu, ZHANG Li-Qiang, WEN Chun-Gen, SUN Jun, GAO Qian. PHYLOGENETIC RELATIONSHIP OF SPECIES IN THE GENUS ASPIDOGASTER (ASPIDOGASTRIDAE, ASPIDOGASTRINAE) IN CHINA AS INFERRED FROM ITS rDNA SEQUENCES[J]. ACTA HYDROBIOLOGICA SINICA, 2010, 34(2): 313-316.
shu

PHYLOGENETIC RELATIONSHIP OF SPECIES IN THE GENUS ASPIDOGASTER (ASPIDOGASTRIDAE, ASPIDOGASTRINAE) IN CHINA AS INFERRED FROM ITS rDNA SEQUENCES

  • College of Fishery, Huazhong Agricultural University, Wuhan 430070, China;
    2. State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China;
    3. Department of Bioscience and Technology, Nanchang University, Nanchang 330047, China;
    4. Institute of Hydrobiology, Jinan University, Guangzhou 510632, China

  • Received Date: July 28, 2008
  • Rev Recd Date: June 13, 2009
  • Published Date: March 24, 2010
    Graphical Abstract
    • Abstract
  • Five species in the genus Aspidogaster have been reported in China, and four species, i.e. A. conchicola Bare, 1827, A. limacoides Diesing, 1834, A. ijimai Kawamura, 1913 and A. chongqingensis Wei, Huang et Dai, 2001, were obtained to examine their phylogenetic relationship by comparing ITS rDNA sequences. The length of the ITS-1 and ITS-2 sequences ranged from 728 to 877 bp and 518 to 645 bp, and the G + C content from 50.1 to 52.3% and 49.2 to 52.2%, respectively. Both maximum likelihood (ML) and neighbour-joining (NJ) phylogenetic trees revealed three well-supported main clades, which correspond respectively to A. limacoides and A. chongqingensis, A. ijimai, and A. conchicola, with A. conchicola located at the base of the phylogenetic tree. The group containing A. chongqingensis and A. limacoides formed the sister taxon to A. ijimai with 100% of bootstrap value. It was considered that A. chongqingensis and A. limacoides should be the closest, and they should be closer to A. ijimai than to A. conchicola. In addition, host specificity of Aspidogaster spp. in China was discussed.
    • FullText(HTML)
    • References (18)
  • [1]
    Rohde K. The origins of parasitism in the Platyhelminthes [J].Int J Parasitol, 1994, 24: 1099—1115.
    [2]
    Rohde K. The minor groups of parasitic Platyhelminthes [J].Adv Parasitol, 1994, 33: 145—234.
    [3]
    Zamparo D, Brooks D. Phylogenetic systematic assessmentof the Aspidobathrea (Platyhelminthes, Neodermata, Trematoda)[J]. Zool Scr, 2003, 32: 83—93.
    [4]
    Bachellerie J P, Qu L H. Ribosomal RNA probes for detectionand identification of species [A]. In: Hyde J E (Ed.),Protocols in Molecular Parasitology [C]. New Jersey: HumanaPress. 1993, 249—264.
    [5]
    Morgan J A, Blair D. Nuclear rDNA ITS sequence variationin the trematode genus Echinostoma: an aid to establishingrelationships within the 37-collar-spine group [J]. Parasitology,1995, 111: 609—615.
    [6]
    Wu B H, Sun X D, Song C C. Fauna of Trematoda in Zhejiangprovince [C]. Hangzhou: Zhejiang Science and TechnologyPublishing House. 1991, 568.
    [7]
    Wei G, Huang L, Dai D L. A new species of aspidogastrids(Trematoda: Aspidogastrea: Aspidogastridae) from fishesof Chongqing, China [J]. Acta Zootax Sin, 2001, 26: 469—470.
    [8]
    Gao Q, Chen M X, Yao W J, et al. Phylogeny of diplozoidsin five genera of the subfamily Diplozoinae Palombi, 1949 asinferred from ITS-2 rDNA sequences [J]. Parasitology, 2007,134: 695—703.
    [9]
    Luton K, Walker D, Blair D. Comparisons of ribosomalinternal transcribed spacers from two congeneric species offlukes (Platyhelminthes: Trematoda: Digenea) [J]. Mol BiochemParasitol, 1992, 56: 323—328.
    [10]
    Thompson J D, Gibson T J, Plewnlak F, et al. Clustal Xwindows interface: flexible strategies for multiple sequencealignment aided by quality analysis tools [J]. Nucleic AcidsRes, 1997, 24: 4876—4882.
    [11]
    Luo H Y, Nie P, Zhang Y A, et al. Molecular variation ofBothriocephalus acheilogathi Yamaguti, 1934 in differentfish host species based on ITS rDNA sequences [J]. SystParasitol, 2002, 52: 159—166.
    [12]
    Dzikowski R, Ievy M C, Poore M F, et al. Use of rDNApolymorphism for identification of heterophyidae infectingfreshwater fishes [J]. Dis Aquat Org, 2004, 59: 35—41.
    [13]
    Kumar S, Tamura K, Nei M. MEGA3: integrated softwarefor molecular evolutionary genetics analysis and sequencealignment [J]. Brief Bioinform, 2004, 5: 150—163.
    [14]
    Felsenstein J. PHYLIP-Phylogeny Inference Package, version3.4. Washington: Department of Genetics, University ofWashington. 1991.
    [15]
    Timofeeva T.A. On the identity of Aspidogaster amurensisAchmerov, 1956 and Aspidogaster conchicola K Baer, 1827(Trematode, Aspidogastrea) [J]. Parazitologiya, 1973, 7:89—90 (In Russian).
    [16]
    Adlard R D, Barker S C, Blair D, et al. Comparison of thesecond internal transcribed spacer (ribosomal DNA) frompopulations and species of Fasciolidae (Digenea) [J]. Int JParasitol, 1993, 23: 423—425.
    [17]
    Cunningham C O. Species variation within the internal transcribedspacer (ITS) region of Gyrodactylus (Monogenea:Gyrodactylidae) ribosomal RNA genes [J]. J Parasitol, 1997,83: 215—219.
    [18]
    Gao Q, Nie P, Yao W J. Scanning electron microscopy ofAspidogaster ijimai Kawamura, 1913 and A. conchicola Baer,1827 (Aspidogastrea, Aspidogastridae) with reference totheir fish definitive-host specificity [J]. Parasitol Res, 2003,91: 439—443.
    • Related Articles

  • Cited by

    Periodical cited type(0)

    Other cited types(1)

Catalog

    Get Citation
    PDF
    XML
    Article views (1000) PDF downloads (719) Cited by(1)
    Related

    Editorial Office of Acta Hydrobiologica Sinica

    Address：No. 7 Donghu South Road, Wuchang District, Wuhan, Hubei Province, China

    Tel：027-68780701；027-68780800 E-mail：acta@ihb.ac.cn  

    Supported byBeijing Renhe Information Technology Co. Ltd  Technical support：info@rhhz.net

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return