Abstract
A Study was conducted to investigate the effects of supplemental vitamin E(VE) in practical diet on growth,meat quality and anti-oxidant capacity of adult grass carp(Ctenopharyngodon idellus).Basal diet,added with VE 0(control group),25,50,100 or 200 mg/kg,were fed to adult grass carp for 60 days.The basal diet contained 28.05% crude protein,3.25% crude fat and 24.1 mg/kg VE.Adult grass carp,with body weight of(614.9?0.5) g,were divided into five treatments with triplicate groups,and raised in 15 cement pools(3.0m?.5m?.2m).Fishes were fed twice per day(8:00 and 17:00) with the feed intake of 2% of the body weight.Growth rate,feed conversion rate(FCR),muscle composition,drip loss of fresh meat,exudative loss of freezing meat,VE and maleic dialdehyde(MDA) in liver and muscle,serum SOD,total antioxidant capacity(T-AOC) were measured.Results showed that growth rates of the five groups were 51.6%,53.5%,53.4%,53.5%,54.8%,and FCR were 2.28,2.17,2.20,2.19,and 2.15,respectively.There was no significantly effect of supplemental VE in diets on growth and FCR of adult grass carp,but growth rate tended to be increased and FCR tended to be decreased by supplemental 200mg/kg VE in diet(P0.10).With supplemental VE increased,the drip loss of fresh meat and exudative loss of freezing meat decreased,but the moisture,crude protein,crude fat of meat kept constant among all groups.The VE concentration of muscle and liver were significantly increased,whereas MDA was decreased by supplemental VE(P0.05).Compared with control group,VE of meat and liver were increased by 431.0%,353.7%,and MDA were decreased by 67.2%,61.3% by supplemental 200mg/kg VE in diet(P0.01).Compared with control group,when supplemental VE was higher than 50 mg/kg,serum SOD activities significantly increased(P0.05),and T-AOC was significantly higher when supplemental VE was 200mg/kg(P0.05).No significant difference in serum T-AOC was observed among groups of 0,50,100 mg/kg VE addition group.Results above showed that growth performance,meat quality and serum anti-oxidant capacity of adult grass carp could be improved by supplemental VE in diets.For adult grass carp,the VE supplement in practical diet(containing VE 24.1 mg/kg) was estimated to be 25 mg/kg according to the growth performance,or 200 mg/kg according to meat quality and anti-oxidant capacity.