CYTOLOGY AND CULTURE OF NEUROSECRETORY CELL IN THE EYESTALK OF ERIOCHEIR SINENSIS

the neurosecretory cells were studied in terms of cytology and primary culture in the MTXO of Eriocheir sinensis, and a practical method for primary culture of peptidergic neurons was set up.The peptidergic neurons, when dissociated from the MTXO, exhibited immediate outgrowth for 3-5 days and survived for 18 days or more in the defined medium supplemented with glutamine and antibiotics.The neurons could survive in some conditions involving changes of pH(7.0- 7.9).temperature(22℃- 28℃) and osmolarity (950- 1100mOsm).The outgrowth of the peptidergic neurons could be restrained in the Ca-free medium and blocked by Cd2+ (Cd2+ current blocker)in the medium.Three types of neurosecretory cells were distinguished on the basis of size, morphology, distribution, form of outgrowth and ultrastructure.There is an ultrastructural evidence that B, C types of neurosecretory cells have a rest phase in the development of Eriocheir sinensis.