LU Kai-hong, LIN Xia. THE EFFECT OF DOMESTICATION WITH SEAWATER AND FRESHWATER ON FATTY ACID COMPOSITION OF FIVE SPECIES OF MICROALGAE[J]. ACTA HYDROBIOLOGICA SINICA, 2001, 25(2): 179-184.
Citation: LU Kai-hong, LIN Xia. THE EFFECT OF DOMESTICATION WITH SEAWATER AND FRESHWATER ON FATTY ACID COMPOSITION OF FIVE SPECIES OF MICROALGAE[J]. ACTA HYDROBIOLOGICA SINICA, 2001, 25(2): 179-184.

THE EFFECT OF DOMESTICATION WITH SEAWATER AND FRESHWATER ON FATTY ACID COMPOSITION OF FIVE SPECIES OF MICROALGAE

  • Received Date: August 18, 1999
  • Rev Recd Date: April 04, 2000
  • Published Date: March 24, 2001
  • The fatty acid composition of five species of microalgae and the effect of domestication with seawater and freshwater on them were studied. The results suggest that each class of microalgae is characterized by a specific fatty acid profile. The specific features of freshwater alga Chlorella Pyrenoidosa, marine algae Chlorella sp.-1 and Chlorella sp.-2 are high content of 16:0, 16:2, 18:0, 18:2 and 18:3; The freshwater alga Scenedesmus obliquus is characterized by high content of 16:0, 18:1 and 18:3; The reliable marks of the marine diatom Phaeodactylum tricornutum are rich in 14:0, 16:0, 16:1, 16:2, 18:0 and 20:5, especially in 16:0 and EPA. Alter domestication, the kind of "characteristic" fatty acids was not changed, but the content of the main fatty acids changed. The content(mg/g cell dry wt)of "characteristic" fatty acids and total fatty acid in C. pyrenoidosa, Chlorella sp.-1 and the clone SS02 of P. tricornutum increased to a certain degree, while the results were opposite in S. obliquus, Chlorella sp.-2 and the clones ZS08, XS03 of P. tricornutum.
  • [1]
    Watanabe T.Nutritional guality of living feed from the viewpoint of EFA for fish [J].Bull.Jpn.Soc.Sci.Fish.,1978,44:1223—1227[2] Yongmanitchal W,Ward O P.Growth of and omega-3 fatty acid production by Phaeodactylum tricornutum under different culture conditions [J].Appl.Environ.Microbiol.,1991,57(2):419—425[3] Al-Hasan R H,Ali A M,Ka'wash H H,et al.Effect of salinity on the lipid and fatty acid composition of the halophyte Navicula sp.:potential in mariculture [J].J.Appl.Phycol.,1990,2:215—222[4] Seto A,Wang H L,Hesseltine C W.Culture conditions affect eicosapentaenoic acid content of Chlorella minutissima [J].J.Am.Oil.Chem.Soc.,1984,61:892—894[5] Teshima S,Yamasaki S,Kanazawa A,et al.Effects of water temperature and salinity on eicosapentaenoic acid level of marine Chlorella [J].Bull.Japn.Soc.Sci.Fish.,1983,49(5):805[6] Renaud S M,Parry D L,Van L,et al.Effects of light intensity on the proximate biochemical and fatty acid composition of Isochrysis sp.and Nannochloropsis oculata for use in tropical aquaculture [J].J.Appl.Phycol.,1991,3:43—53[7] Yongmanitchal W,Ward O P.Screening of algae for potential alternative sources of eicosapentaenoic acid [J].Phytochemistry,1991,30(9):2963—2967[8] Kochert G.Quantitation of the macromolecular components of microalgae.Physiological and Biochemical methods [M].London:Cambridge University Press,1978,189[9] Ackman R G.Simplification of analysis of fatty acids in fish lipids and related lipid samples [J].Acta.Med.Scand,1987,222:99—103[10] Milner H W.The fatty acid of Chlorella [J].J.Biochem.,1948,176:813—817[11] Ackman R G,Tocher C S,Melachlan J.Marine phytoplankter fatty acids [J].J.Fish.Res.Board Can.,1968,25:1603—1620[12] Volkman J K,Jeffrey S W,Nichols P D,et al.Fatty acid and lipid composition of 10 species of microalgae used in mariculture [J].J.Exp.Mar.Biol.Ecol.,1989,128:219—240[13] Zhukova N V,Aizdaicher N A.Fatty acid composition of 15 species of marine microalgae [J].Phytochemistry,1995,39(2):351—356[14] 李荷芳,周汉秋.海洋微藻脂肪酸组成的比较研究 [J].海洋与湖沼,1999,30(1):34—40

    Watanabe T.Nutritional guality of living feed from the viewpoint of EFA for fish [J].Bull.Jpn.Soc.Sci.Fish.,1978,44:1223—1227[2] Yongmanitchal W,Ward O P.Growth of and omega-3 fatty acid production by Phaeodactylum tricornutum under different culture conditions [J].Appl.Environ.Microbiol.,1991,57(2):419—425[3] Al-Hasan R H,Ali A M,Ka'wash H H,et al.Effect of salinity on the lipid and fatty acid composition of the halophyte Navicula sp.:potential in mariculture [J].J.Appl.Phycol.,1990,2:215—222[4] Seto A,Wang H L,Hesseltine C W.Culture conditions affect eicosapentaenoic acid content of Chlorella minutissima [J].J.Am.Oil.Chem.Soc.,1984,61:892—894[5] Teshima S,Yamasaki S,Kanazawa A,et al.Effects of water temperature and salinity on eicosapentaenoic acid level of marine Chlorella [J].Bull.Japn.Soc.Sci.Fish.,1983,49(5):805[6] Renaud S M,Parry D L,Van L,et al.Effects of light intensity on the proximate biochemical and fatty acid composition of Isochrysis sp.and Nannochloropsis oculata for use in tropical aquaculture [J].J.Appl.Phycol.,1991,3:43—53[7] Yongmanitchal W,Ward O P.Screening of algae for potential alternative sources of eicosapentaenoic acid [J].Phytochemistry,1991,30(9):2963—2967[8] Kochert G.Quantitation of the macromolecular components of microalgae.Physiological and Biochemical methods [M].London:Cambridge University Press,1978,189[9] Ackman R G.Simplification of analysis of fatty acids in fish lipids and related lipid samples [J].Acta.Med.Scand,1987,222:99—103[10] Milner H W.The fatty acid of Chlorella [J].J.Biochem.,1948,176:813—817[11] Ackman R G,Tocher C S,Melachlan J.Marine phytoplankter fatty acids [J].J.Fish.Res.Board Can.,1968,25:1603—1620[12] Volkman J K,Jeffrey S W,Nichols P D,et al.Fatty acid and lipid composition of 10 species of microalgae used in mariculture [J].J.Exp.Mar.Biol.Ecol.,1989,128:219—240[13] Zhukova N V,Aizdaicher N A.Fatty acid composition of 15 species of marine microalgae [J].Phytochemistry,1995,39(2):351—356[14] 李荷芳,周汉秋.海洋微藻脂肪酸组成的比较研究 [J].海洋与湖沼,1999,30(1):34—40

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