FANG Hai, CHEN Cui-Zhen ZHANG, Xiao-Jun. STUDIES ON BIOLOGICAL PROPERTIES OF VIBRIO QINHUANGDAORA SP. NOV. ISOLATED FROM DISEASED FLOUNDER PARALICHTHYS OLIVACEU S[J]. ACTA HYDROBIOLOGICA SINICA, 2005, 29(4): 366-371.
Citation: FANG Hai, CHEN Cui-Zhen ZHANG, Xiao-Jun. STUDIES ON BIOLOGICAL PROPERTIES OF VIBRIO QINHUANGDAORA SP. NOV. ISOLATED FROM DISEASED FLOUNDER PARALICHTHYS OLIVACEU S[J]. ACTA HYDROBIOLOGICA SINICA, 2005, 29(4): 366-371.

STUDIES ON BIOLOGICAL PROPERTIES OF VIBRIO QINHUANGDAORA SP. NOV. ISOLATED FROM DISEASED FLOUNDER PARALICHTHYS OLIVACEU S

  • Received Date: July 05, 2004
  • Rev Recd Date: August 24, 2004
  • Published Date: July 24, 2005
  • Pathological bacteriawere isolated from diseased flounder sufferd bacterial septicaemic infection in Qinhuangdao. Iden-tification of morphological, culturing, physiological and biochemical characteristics to 12 pure cultures and detection of themol%G+ C ratio of the DNA to representative strain, showed that the pathogenic bacteria belonged to a new species of Vibrio (pacini 1854) and were designated as Vibrio qinhuangdaora sp.nov. Simultaneously, we conducted examinations on thehomologyof serum, the results showed that the tested 12 strains have the same O-antigen and the sameK-antigen.In addition, the suscept-ibilities of isolates to 37 antimicrobial agents were detected, the results showed that all the strains were sensitive to cefazolin etal.,were resistant to penicillin G et al., and there isn. t obvious discrepancy of sensitivity and resistance between strains.
  • [1]
    Wang G L, Xue L Y, Jin S, et al. Epidemiological studies on the skinulcer disease of Lateolabrax japonicus in marine cage culture[J]. ActaHydrobiologica sinica, 2000, 24(4): 392-395[王国良, 薛良义, 金珊, 等. 海水网箱养殖鲈鱼皮肤溃疡病的流行病学研究, 水生生物学报,2000, 24(4): 392-395]
    [2]
    Austin B, Austin D A. Bacterial Fish Pathogens: Disease of Farmedand Wild Fish[M].Third (Revised-Edition. Praxis Publishing Ltd,Chichester,UK. 1999, 29-32, 106-140
    [3]
    Zhang X J, Zhan W B, Chen C Z, et al. Studies on the infection andpathogen of Edward siella tarda in flounder Paralichthys olivaceus[J]. ActaHydrobiol. sin.,2005, 29(1): 31-37[张晓君, 战文斌,陈翠珍, 等. 牙鲆迟钝爱德华氏菌感染症及其病原的研究, 水生生物学报,2005, 29(1):31-37]
    [4]
    Shen J Y, Yin W L, Qian D, et al. Studies on pathogens of bacterialdiseases of Eriocheir sinensis[J]. Journal of fishery sciences of China.2000, 7(3): 89-92[沈锦玉, 尹文林, 钱冬, 等. 中华绒螯蟹/ 腹水病0及/ 抖抖病0并发病病原的研究, 中国水产科学, 2000, 7(3): 89-92]
    [5]
    Ma JM, Wang Q,Ma F H, et al. A Pathogen of septicopyaemia in theabalone Haliotis discus hannai ino. Dalian, China [J]. Journal ofFisheries of China 1996, 20(4): 332-336[马建民, 王琦, 马福恒,等. 皱纹盘鲍脓毒败血症病原菌的发现及初步研究, 水产学报, 1996, 20(4): 332-336]
    [6]
    Wang G L, Zheng T L, JinS, et al. Preliminary studies on pathogen ofascites disease of cultured Sparus macrocephelus[J]. Chin J Vet Sci,2003, 23(1): 33-35[王国良, 郑天伦, 金珊, 等. 黑雕幼鱼腹水病病原菌, 中国兽医学报, 2003, 23(1): 33-35]
    [7]
    Xu B F, Lin N F, Yang J X, et al. Isolation identification andpathogenicity analysis of Vibrio parahaemolyticus from pseudosciaenacrocea[J] Fujian Joumal of Agricultural Sciences, 2002, 17(3): 174)177[许斌福, 林能锋, 杨金先, 等. 大黄鱼副溶血弧菌的分离、鉴定及致病力分析, 福建农业学报, 2002,17(3): 174-177]
    [8]
    Anguianobltran R, Searcybernal M L, Lizarragapartida, et al. Pathogeniceffects of Vibrio alginolyticus on Larvae and postlarvae of the red adalone[J]. Diseases of Aquatic Organisms, 1998,33(2):112-119
    [9]
    Zhang X H, Austin B. Pathogenicity of Vibrio harveyi to salmonids[J]. Journal Fish Disease, 2000, 23: 93-102
    [10]
    Mo Z L,Mao Y X, Chen S Y, et al. Classification for one pathogenicVibrio anguillarum strain isolated from skin ulcer flounder[J]. ActaMicrobiologica Sinica, 2002, 42(3): 263-269[莫照兰, 茅云翔, 陈师勇,等. 一株牙鲆皮肤溃烂症病原菌的鉴定, 微生物学报,2002, 42(3): 263-269]
    [11]
    Mo Z L,Mao Y X, Chen S Y, et al. Classification and molecular bio -logical analysis of one pathogenic bacterium associated with hemor -rhages of cultured flounder[J]. High technology tetters, 2001, 12:12-17[莫照兰, 茅云翔, 陈师勇, 等. 一株牙鲆出血症病原菌的分子生物学鉴定, 高技术通讯, 2001, 12: 12-17]
    [12]
    Mo Z L, Mao Y X, Chen S Y, et al. Identification and phylogeneticanalysis of one pathogenic bacterium associated with swollen abdomenof cultured flounder(Paralichthys olivaceus)larvae[J]. Oceanologiaetlimnologia sinica, 2003,34(2): 131-141[莫照兰, 茅云翔, 陈师勇, 等. 养殖牙鲆鱼苗腹水症病原菌的鉴定及系统发育学分析, 海洋与湖沼, 2003, 34(2): 131-141]
    [13]
    Chang J B, Gong X H, Sun F X, et al. Preliminary studies on apathogen of vibriosis of cultured Paralichthys olivaceus [J]. Marinefisheries research, 2001, 22(1): 37-41[常建波, 宫向红, 孙逢贤,等. 养殖牙鲆弧菌病病原菌初步研究, 海洋水产科学, 2001, 22(1): 37-41]
    [14]
    Wu H B, Pan J P. Progress in studies of vibriosis in aquaculture[J].Journal of fishery sciences of China.2001, 8(1): 89-93[吴后波, 潘金培. 弧菌属细菌及其所致海水养殖动物疾病, 中国水产科学, 2001, 8(1): 89-93]
    [15]
    Yang Z S, Fang H. Human and animal pathogenic bacteriology[M].Shijiazhuang: Hebei Science and Technology Press, 2003, 1403)1421, 1523-1643[杨正时, 房海. 人及动物病原细菌学. 石家庄: 河北科技出版社,2003, 1403-1421,1523-1643]
    [16]
    Krieg N R,Holt J G. Bergeyps Manual of Systematic Bacteriology[M].Volume 1. London:Williams and Wilkins, Baltimore, 1984, 518-538
    [17]
    Holt J G, Krieg N R, Sneath P H A, et al. Bergeyps Manual of Deter -minative Bacteriology [M]. Ninth Edition. Baltimore, Williams andWilkins, 1994, 190-194, 253-274
    [18]
    Ye Y W, Wang Y S. Operating specification for clinical examination ofnational(2th ed-[M]. Nanjing: Southeast university press, 1997,553-562[叶应妩, 王毓三. 全国临床检验操作规程(第二版),南京: 东南大学出版社, 1997, 553-562]

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