CLONING OF MYOSTATIN OF COMMON CARP(CYPRINU S CARPIO) AND ITS EXPRESSION PATTERN IN DIFFERENT TISSUES

LI Xing-Mei; FAN Wei; ZHANG Bin; PENG Kou; WANG Yu-Feng; HU Wei; ZHAO Hao-Bin

Volume 31 Issue 5

Aug. 2014

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ACTA HYDROBIOLOGICA SINICA > 2007 > 31(5): 643-648.

LI Xing-Mei, FAN Wei, ZHANG Bin, PENG Kou, WANG Yu-Feng, HU Wei, ZHAO Hao-Bin. CLONING OF MYOSTATIN OF COMMON CARP(CYPRINU S CARPIO) AND ITS EXPRESSION PATTERN IN DIFFERENT TISSUES[J]. ACTA HYDROBIOLOGICA SINICA, 2007, 31(5): 643-648.

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CLONING OF MYOSTATIN OF COMMON CARP(CYPRINU S CARPIO) AND ITS EXPRESSION PATTERN IN DIFFERENT TISSUES

School of Life Sciences, Huazhong Normal University, Wuhan 430079;
2. State Key Laboratory ofFreshwater Ecology and Biotechnology, Institute of Hydrobiology, the Chinese Academy of Sciences, Wuhan 430072

Received Date: October 25, 2005
Rev Recd Date: December 20, 2006
Published Date: September 24, 2007

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Abstract

Myostatin (MSTN) is the negative regulator in themuscular development, and the studies ofMSTN will benefit the animal production? We cloned the partial sequences of MSTN cDNA (Gene Bank #: EF551058) of common carp, a major aquaculture fish in China, by reverse transcription-polymerase chain reaction (RT-PCR) with degenerate primers? The partial sequence of carp MSTN consists of 921 base pairs which encode 306 amino acids? The translation of the partial sequence of carp MSTN shows the common characterization ofMSTNs, it has a predomain and a mature MSTN domain with conservative hydrolytic site, RIRR, and 9 cysteineresidues? The carp MSTN has high homology of 96?7% with zebrafish (Danio rerio) GDF8? The expression pattern of carp MSTN was detected with RT-PCR in different tissues of adult fish, the results showed that the carp MSTN only could be detected in muscle and brain, we did not obtaine PCR results in other tissues detected? The expression pattern of MSTN indicated that carpMSTN could play important role in muscular development, and it could play some role in neural development?
- Common carp (Cyprinus carpio),
- Myostatin,
- Cloning,
- Homology,
- Expression pattern

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CLONING OF MYOSTATIN OF COMMON CARP(CYPRINU S CARPIO) AND ITS EXPRESSION PATTERN IN DIFFERENT TISSUES

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