KARYOTYPE EVIDENCES OF GENOME MANIPULATION IN THE CARP(CYPRINUS CARPIO L.)

This paper presents karyotype evidences of induced haploid, gynogenetic diploid and triploid carp by means of genome manipulation. Karyotype analysis of the haploid shows that the complement of chromosome consists of 6 metacentrics(M), 20 submetacentrics(SM) and 24 subtelocentries and telocentrics(ST and T). It demonstrates that chromosome components of gynogenetic diploid were derived from two complete genomes. Hence, with normal viability and functional ovary, the second gynogenetic generation is able ta be employed as a stock for establishing a pure line. The chromosome complement of some triploid individuals having normal functional ovaries consists of three complete genomes. This evidence suggests that the major reason of the sterility of most induced triploid fish might result from aneuroploid or breakage of chromosomes during temperature shock.