IMMUNOCYTOCHEMICAL LOCALIZATION OF NINE TELEOSTS USING MONOCLONAL ANTIBODY OF GRASS CARP GROWTH HORMONE

using immunocytochemical technique of Strept Avidin Biotin-peroxidaseComplex (SABC), growth hormone (GH) immunoreactive endocrine cells in thegastro-entero-pancreas of northern snakehead (Channa argus), largemouth bass(Micropterus salmoides), oriental sheatfish (Silurus asotus), yellow catfish (Pelteobagrusfulvidraco), mandarin fish (Siniperca chuatsi), ricefield eel (Monopterus albus),freshwater pomfret(Colossoma brachypomum), and nile tilapia (Oreochromis nilotica)were located with monoclonal antiserum of grass carp GH raised against mouse.Usingthe same method, immunocytochemical localization of the hypophysis of grass carpwere investigated with grass carp GH antiserum and somatostation (Som) antiserum.Results showed that many GH immunoreactive endocrine cells existed in the islets ofLangerhans of S.chuatsi and S. asotus, and no positive specificity specificity reaction was found in all digestive tract of ve tract of 8 teleosts GH- and Som-immunoreactive endocrine cells werefound in the proadenohypophysis and meso-adenohypophysis of grass carp.