ZHANG Jin-Yan, XI Yi-Long, MA Qin, XIANG Xian-Ling. TAXONOMICAL STATUS OF TWO BRACHIONUS CALYCIFLORUS MORPHOTYPES IN LAKE LIANTANG BASED ON ITS SEQUENCE[J]. ACTA HYDROBIOLOGICA SINICA, 2010, 34(5): 935-942.
Citation: ZHANG Jin-Yan, XI Yi-Long, MA Qin, XIANG Xian-Ling. TAXONOMICAL STATUS OF TWO BRACHIONUS CALYCIFLORUS MORPHOTYPES IN LAKE LIANTANG BASED ON ITS SEQUENCE[J]. ACTA HYDROBIOLOGICA SINICA, 2010, 34(5): 935-942.

TAXONOMICAL STATUS OF TWO BRACHIONUS CALYCIFLORUS MORPHOTYPES IN LAKE LIANTANG BASED ON ITS SEQUENCE

  • Received Date: July 09, 2009
  • Rev Recd Date: April 25, 2010
  • Published Date: September 24, 2010
  • Cyclomorphosis of rotifer defines the temporal cyclic morphological changes that occur within a rotifer population, including variation in body size, presence or absence and lengths of lateral spines and posteriolateral spines. This widespread phenomenon has complicated rotifer taxonomy. The study on genetic differentiation among different rotifer morphotypes by using molecular markers can help us to recognize their taxonomical status correctly. In the pre-sent study, the ITS sequences of 30 (S1-S30) spined and 18 (U1-U18) unspined Brachionus calyciflorus clones, col-lected from Lake Liantang, were analyzed, and their molecular phylogenetic trees were constructed by Neighbor-Joining (NJ), Maximum-Parsimony (MP), Maximum-Likelihood (ML) and Bayesian methods using B. patulus as an outgroup. The results showed that all the 48 clones belonged to 16 haplotypes. Within ITS sequence, the average content of T, C, A and G base was 28.6%, 18.7%, 35.9% and 16.8%, respectively. The content of A base plus T base was 64.5%, but that of C base plus G base was 35.5%. The sequence divergence was 26.2%-26.6% between haplotype U12 and the other haplotypes, with an average of 26.47%. The sequence divergence occurred in ITS1, 5.8S and ITS2 was 26.9%-27.8%, 2.9%-3.5% and 44.4%-45.0%, and the mean value was 27.27%, 3.09% and 44.48%, respectively. However, the av-erage sequence divergence was 0.41% among the other haplotypes. All the four phylogenetic trees supported that 48 clones should be obviously divided into two different clades. The first clade included clone U12, and the second one included all the spined clones and the other unspined clones. Haplotype U12 and the other haplotypes belonged to two sibling species, but the two B. calyciflorus morphotypes were not different subspecies or sibling species, and their variation in morphology attributed mainly to phenotypic plasticity.
  • [1]
    Black R W,Siobodkin L B.What is cyclomorphosis[J]?Freshwater Biology,1987,18:373-378
    [2]
    Serra M,Galiana A,Gómez A.Speciation in monogonont rotifers[J].Hydrobiologia,1997,358:63-70
    [3]
    Gilbert J J.Mictie female production in rotifer Brachionus calyciflorus[J].Journal of Experimental Zoology,1963,153:113-124
    [4]
    Hu H Y,Xi Y L,Geng H.Effects of temperature on life history strategies of three strains of Brachionus angularis gosse[J].Acta Hydrobiologica Sinica,2004,28(3):284-288[胡好远,席贻龙,耿红.温度对三品系角突臂尾轮虫生活史策略的影响.水生生物学报,2004,28(3):284-288]
    [5]
    Koste W.Rotatoria.Die Radertiere Mitteleuropas[M].Borntraeger,Berlin.1978,pp 673
    [6]
    Derry A M,Hebert P D N,Prepas E E.Evolution of rotifers in saline and subsaline lakes:A molecular phylogenetic approach[J].Limnoloy & Oceanography,2003,48(2):675-685
    [7]
    Fellisen R S.Comparative sequence analysis of the 5.8S rDNA genes and internal transcribed spacer(ITS)regions of trichomonadid protozoa[J].Parasitology,1997,115:111-119
    [8]
    Gómez A,Serra M,Carvalho G R,et al.Speciation in ancient cryptic species complexes:evidence from the molecular phylogeny of Brachionus plicatilis(Rotifera)[J].Evolution,2002,56(7):1431-1444
    [9]
    Tang B P,Zhou K Y,Song D X.Application of sequences of nrDNA ITS to molecular systematies of invertebrates[J].Chinese Journal of Zoology,2002,37(4):67-73[唐伯平,周开亚,宋大祥.核rDNA ITS序列在无脊椎动物分子系统学研究中的应用.动物学杂志,2002,37(4):67-73]
    [10]
    Xi Y L,Chen Y Q,Zhuge Y,et al.Sequence analysis of rDNA 18S-28S intergenic spacer regions from Brachionus calyciflous,B.bidentata,B.diversicornis and B.angularis in Lake Donghu,China[J].Acta Hydrobiologica Sinica.2003,27(4):427-430[席贻龙,陈月琴,诸葛燕,等.四种臂尾轮虫rDNA 18S-28S基因间隔区的序列测定与分析.水生生物学报,2003,27(4):427-430]
    [11]
    Gilbert J J,Walsh E J.Brachionus calyciflorus is a species complex:mating behavior and genetic differentiation among four geographically isolated strains[J].Hydrobiologia,2005,546:257-265
    [12]
    Xiang X L,Xi Y L,Hu H Y.Phyiogenetic relationships of Brachionus rotifers based on rDNA ITS 1 gene sequences[J].Acta Zoologica Sinica,2006,52(6):1067-1074
    [13]
    Zhang Z S,Huang X F.Study Methods on Freshwater Plankton[M].Beijing:Science Press.1991[章宗涉,黄祥飞.淡水浮游动物研究方法.北京:科学出版社,1991]
    [14]
    Hao J S,Yang Q,Li C X,et al.Molecular phylogeny of Lophotrochozoa based on 18S rRNA gene sequences-with comment on the phylogenetic position of Bryozoa[J].Journal of Genetics and Molecular Biology,2003,14(2):64-72
    [15]
    Thompson J D,Higgins D G,Gibson T J.CLUSTAL X multiple sequence alignment program[M].Version 1.8.1999
    [16]
    Kumar S,Tamura K,Nei M.MEGA3:Integrated software for molecular evolutionary genetics analysis and sequence alignment[J].Briefings in Bioinformatics,2004,5:150-163
    [17]
    Xia X H,Xie Z.DAMBE:data analysis in molecular biology and evolution[J].Journal of heredity,2001,92:371-373
    [18]
    Posada D,Crandall K A.Modeltest:testing the model of DNA substitution[J].Bioinformatics,1998,9:817-818
    [19]
    Ronquist F,Huelsenbeck J P.MRBAYES 3:Bayesian pbylogenetic inference under mixed models[J].Bioinformatics,2003,19:1572-1574
    [20]
    Gómez A,Snell T W.Sibling species and cryptic speciation in the Brachionus plicatilis species complex(Rotifera)[J].Journal of Evolutionary Biology,1996,9:953-964
    [21]
    Li H B,Xi Y L,Cheng X F,et al.Sympatric speciation in rofifers:evidence from molecular phylogenetic relationships and reproductive isolation among Brnchionus calyciflous clones[J].Acta Zoologica Sinica,2008,54(2):256-264[李化炳,席贻龙,程新峰,等.轮虫同域性物种形成:来自萼花臂尾轮虫克隆间的分子系统发育关系和生殖隔离证据.动物学报,2008,54(2):256-264]
    [22]
    Cheng X F,Xi Y L,Li H B.Seasonal changes in the genetic structure of a Brachionus calyciflous population in Lake Liantang based on ITS sequences[J].Acta Zoologica Sinica,2008,54(2):245-255[程新峰,席贻龙,李化炳.基于rDNA ITS序列分析莲塘湖萼花臂尾轮虫种群遗传结构的季节变化.动物学报,2008,54(2):245-255]
    [23]
    Pennak R W.Rotifera(Rotifers).In:Pennak R W(Eds.),Freshwater invertebrates of the United States:Protozoa to mollusca[M].3rd ed.Wiley.1989,169-225
    [24]
    Gilbert J J.Asplanchna and posterolateral spine induction in Brachionus calyciflorus[J].Archiv Für Hydrobiologie,1967,64:1-62
    [25]
    Gilbert J J,Stemberger R S.Asplanchna-induced polymorphism in the rotifer Keratella slacki[J].Limnoloy & Oceanography,1984,29(6):1309-1316
    [26]
    Gilbert J J.Spine development in Brachionus quadridentatus from an Australian billabong:genetic variation and induction by Asplanchna[J].Hydrobiologia,2001,19(28):446-447
    [27]
    Halbach U.Die Ursachen der temporaivarintion von Brachionus calyciflorus Pallas(Rotiferla)[M].Oecologia,1970,4:262-318
    [28]
    Stemberger R S,Gilbert J J.Spine development in the rotifer Keratella cochlearis:induction by cyclopoid copepods and Asplanehna[J].Freshwater Biology,1984,14:639-647
    [29]
    Bradshaw A D.Evolutionary significance of phenotypic plasticity in plants[J].Advances in genetics,1965,13:115-155
    [30]
    Sultan S E.Phenotypie plasticity for plant development,function and life history[J].Trends in Plant Science,2000,5:537-542
    [31]
    Schlichting C D,Smith H.Phenotypic plasticity:linking molecular mechanisms with evolutionary outcomes[J].Evolutionary Ecology,2002,16:189-211
    [32]
    Pigliucci M,Murren C J,Schlichting C D.Phenotypic plasticity and evolution by genetic assimilation[J].Journal of Experimental Biology,2006,209:2362-2367
    [33]
    Richards E J.Inherited epigenetic variation-revisiting soft inheritance[J].Nature Reviews Genetics,2006,7:395-401
  • Related Articles

    [1]XU Qing-Lai, ZHOU Ji-Shu, ZHU Zi-Lin, WANG Ai, ZHANG Nian-Kun, HE Hua. COMPOUND CHINESE HERBAL MEDICINE ON FLESH QUALITY OF LARGEMOUTH BASS (MICROPTERUS SALMOIDES) IN THE DIET OF FISH MEAL REPLACED BY CHLORELLA MEAL[J]. ACTA HYDROBIOLOGICA SINICA, 2024, 48(7): 1245-1257. DOI: 10.7541/2024.2024.0016
    [2]YOU Cui-Hong, LI Hong-Wei, ZHOU Meng, LIN Li, DONG Ye-Wei, TAN Xiao-Hong, HUANG Yan-Hua. REPLACING DIFFERENT PROPORTIONS OF FISHMEAL WITH HERMITIA ILLUCENS MEAL ON THE GROWTH, HEALTH AND MUSCLE QUALITY OF MICROPTERUS SALMOIDES[J]. ACTA HYDROBIOLOGICA SINICA, 2024, 48(3): 372-383. DOI: 10.7541/2024.2023.0159
    [3]XIAO Yang-Bo, CAO Shen-Ping, AO Qing, HUANG Kang, MO Yu-Jian, ZHANG Xin-Ran, ZHENG Xin-Yi, TONG Xiao-Nian, MAO Zhuang-Wen, FAN Jun-De, LIU Zhen, TANG Jian-Zhou. REPLACING FISH MEAL WITH HERMETIA ILLUCENS LARVAE MEAL ON GROWTH PERFORMANCE, DIGESTIVE CAPACITY, PLASMA BIOCHEMICAL INDEXES AND RELATED GENES EXPRESSION IN HEFANG CRUCIAN CARP[J]. ACTA HYDROBIOLOGICA SINICA, 2023, 47(9): 1363-1373. DOI: 10.7541/2023.2022.0476
    [4]FU Peng, YUE Hua-Mei, RUAN Rui, YE Huan, LI Zhong, LI Chuang-Ju. ANTARCTIC KRILL MEAL REPLACEMENT OF FISH MEAL ON GROWTH PERFORMANCE AND FECUNDITY OF FEMALE MONOPTERUS ALBUS[J]. ACTA HYDROBIOLOGICA SINICA, 2023, 47(2): 249-256. DOI: 10.7541/2023.2022.0353
    [5]GAO Wen-Hao, PENG Shu-Feng, LIAO Chun-Yan, DONG Xiao-Hui, TAN Bei-Ping, WANG Jia, CHI Shu-Yan. METHANOTROPH (METHYLOCOCCUS CAPSULATUS, BATH) BACTERIA MEAL REPLACEMENT OF FISHMEAL ON GROWTH, LIPID DEPOSITION AND ANTIOXIDANT CAPACITY OF TRACHINOTUS OVATUS[J]. ACTA HYDROBIOLOGICA SINICA, 2023, 47(2): 195-203. DOI: 10.7541/2023.2022.0245
    [6]ZHOU Lu-Yang, WU Dai-Wu, GAO Min-Min, HE Jie, SUN Fei, YU Nong, YE Yuan-Tu, CAI Chun-Fang, WU Ping, TANG Feng, PU Qin-Hua, REN Sheng-Jie. THE EFFECTS OF FISH MEAL REPLACEMENT ON GROWTH PERFORMANCE OF YELLOW CATFISH (PELTEOBAGRUS FULVIDRACO)[J]. ACTA HYDROBIOLOGICA SINICA, 2019, 43(3): 504-516. DOI: 10.7541/2019.062
    [7]YANG Jing-Feng, HUA Xue-Ming, GUO Zi-Hao, LIU Tao, KONG Chun, FENG Yue, WANG Gang. THE REPLACEMENT OF FISH MEAL AND SOYBEAN MEAL TO FERMENTED SOYBEAN MEAL AND ITS EFFECTS ON THE GROWTH PERFORMANCE, SERUM BIOCHEMICAL INDICES, AND IMMUNE GENE EXPRESSION IN GIANT RIVER PRAWN, MACROBRACHIUM ROSENBERGII[J]. ACTA HYDROBIOLOGICA SINICA, 2018, 42(4): 719-727. DOI: 10.7541/2018.088
    [8]LIU Xiao-Qing, ZHU Xiao-Ming, HAN Dong, JIN Jun-Yan, YANG Yun-Xia, XIE Shou-Qi. EFFECTS OF DIETARY RATIO OF FISH MEAL AND RAPESEED MEAL ON THE GROWTH AND FEED UTILIZATION IN GIBEL CARP(CARASSIUS AURATUS GIBELIO)[J]. ACTA HYDROBIOLOGICA SINICA, 2014, 38(4): 657-663. DOI: 10.7541/2014.93
    [10]ZHOU Meng, CUI Yi-bo, ZHU Xiao-ming, LEI Wu, YANG Yun-xia, XIE Shou-qi. EFFECT OF REPLACEMENT OF FISH MEAL BY SOYBEAN MEAL AND POTATO PROTEIN CONCENTRATE IN THE DIET FOR GIBEL CARP ON GROWTH AND ENERGY BUDGET[J]. ACTA HYDROBIOLOGICA SINICA, 2002, 26(4): 370-377.

Catalog

    Article views (1086) PDF downloads (519) Cited by()
    Related

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return